The
 adcA
 and
 lmb
 Genes Play an Important Role in Drug Resistance and Full Virulence of
 Streptococcus suis

Peng, Mingzheng; Xu, Yuanyuan; Dou, Beibei; Yang, Fengming; He, Qiyun; Liu, Zewen; Gao, Ting; Liu, Wei; Yang, Keli; Guo, Rui; Li, Chang; Tian, Yao; Zhou, Danna; Bei, Weicheng; Yuan, Fangyan

doi:10.1128/spectrum.04337-22

Cited by 3 publications

(3 citation statements)

References 67 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed that the absence of the Lgt and Lsp leads to a decrease in biofilm formation for both ST1 and ST25 strains, suggesting that LPPs are indeed involved in this process. Accordingly, AdcACB and Lmb, two zinc-binding LPPs, have already been described as implicated in S. suis biofilm formation [42]. However, the technique used in our studies only allows us to observe the overall behavior of the biofilm.…”

Section: Discussionmentioning

confidence: 94%

“…Biofilm formation by pathogenic microorganisms is a mechanism that allows them to become persistent colonizers and resist clearance by the innate and adaptive host immune system [36,41]. Since the involvement of LPPs in S. suis biofilm formation has been suggested [42], the ST1 and ST25 wild-type strains and their respective Δlgt and Δlsp mutant were tested using the crystal violet assay. Results confirmed that the wild-type ST1 strain produced more biofilm than the wild-type ST25 (Figure 3A,B) [43].…”

Section: Lgt and Lsp Enzymes Are Important For S Suis Biofilm Formati...mentioning

confidence: 99%

See 1 more Smart Citation

Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain

Payen,

Roy,

Okura

et al. 2023

Pathogens

View full text Add to dashboard Cite

Streptococcus suis serotype 2 is an important swine bacterial pathogen causing sudden death, septic shock, and meningitis. However, serotype 2 strains are phenotypically and genotypically heterogeneous and composed of a multitude of sequence types (STs) whose distributions greatly vary worldwide. It has been previously shown that the lipoprotein (LPP) maturation enzymes diacylglyceryl transferase (Lgt) and signal peptidase (Lsp) significantly modulate the inflammatory host response and play a differential role in virulence depending on the genetic background of the strain. Differently from Eurasian ST1/ST7 strains, the capsular polysaccharide of a North American S. suis serotype 2 ST25 representative strain only partially masks sub-capsular domains and bacterial wall components. Thus, our hypothesis is that since LPPs would be more surface exposed in ST25 strains than in their ST1 or ST7 counterparts, the maturation enzymes would play a more important role in the pathogenesis of the infection caused by the North American strain. Using isogenic Δlgt and Δlsp mutants derived from the wild-type ST25 strain, our studies suggest that these enzymes do not seem to play a role in the interaction between S. suis and epithelial and endothelial cells, regardless of the genetics background of the strain used. However, a role in the formation of biofilms (also independently of the STs) has been demonstrated. Moreover, the involvement of LPP dendritic cell activation in vitro seems to be somehow more pronounced with the ST25 strain. Finally, the Lgt enzyme seems to play a more important role in the virulence of the ST25 strain. Although some differences between STs could be observed, our original hypothesis that LPPs would be significantly more important in ST25 strains due to a better bacterial surface exposition could not be confirmed.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Lgt and Lsp Enzymes Are Important For S Suis Biofilm Formati...mentioning

confidence: 99%

Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain

Payen,

Roy,

Okura

et al. 2023

Pathogens

View full text Add to dashboard Cite

show abstract

“…The construction of a thyA gene deletion strain of S. suis HA9801 was performed according to the procedure previously described [21]. The upstream and downstream regions of the thyA gene were amplified using thyA-A/thyA-B and thyA-C/thyA-D primers, respectively, by Polymerase Chain Reaction (PCR) (Table 2).…”

Section: Construction Of Thya Deletion Mutant Strain Of S Suismentioning

confidence: 99%

Tackling Antibiotic Resistance: Exploring 5-Fluorouracil as a Promising Antimicrobial Strategy for the Treatment of Streptococcus suis Infection

Zuo,

Quan,

et al. 2024

Animals

View full text Add to dashboard Cite

Streptococcus suis (S. suis) is a zoonotic pathogen with a global distribution, which causes serious diseases in both humans and animals and economic losses in the swine industry. As antibiotic resistance increases, there is an urgent imperative to explore novel antibacterial alternatives. In the present study, we selected the anticancer drug 5-fluorouracil (5-FU) approved by the Food and Drug Administration (FDA) as a candidate drug to treat S. suis infections. The results showed that various pathogens, especially S. suis, are more sensitive to 5-FU. Moreover, the cytotoxicity of 5-FU is relatively low. Extensive in vitro assays demonstrated the pronounced bacteriostatic and bactericidal efficacy of 5-FU against susceptible and multidrug-resistant S. suis strains. Its mechanisms of action include damage to the bacterial cell walls and membranes, resulting in the leakage of intracellular components, and the inhibition of thymidylate synthase (TS), leading to a depletion of deoxythymidine triphosphate (dTTP) pools, ultimately causing thymine-less death and lethal DNA damage in bacteria. Gene-knockout experiments further showed that 5-FU played a role by inhibiting the thyA gene-encoding thymidine synthase. Finally, we determined that S. suis infections can be alleviated by 5-FU in the mouse infection model. This study emphasizes the antibacterial potential of 5-FU against S. suis and provides evidence for its targeting of bacterial membrane damage and DNA damage. In summary, 5-FU can control S. suis infection and is expected to become a new alternative to antibiotics.

show abstract

Rethinking the control of Streptococcus suis infection: Biofilm formation

Wang,

Fan,

Wang

et al. 2024

Veterinary Microbiology

View full text Add to dashboard Cite

The adcA and lmb Genes Play an Important Role in Drug Resistance and Full Virulence of Streptococcus suis

Abstract: Transition metals are important micronutrients for bacterial growth. Zn is necessary for the catalytic activity and structural integrity of various metalloproteins involved in bacterial pathogenic processes.

Cited by 3 publications

References 67 publications

Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain

Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain

Tackling Antibiotic Resistance: Exploring 5-Fluorouracil as a Promising Antimicrobial Strategy for the Treatment of Streptococcus suis Infection

Rethinking the control of Streptococcus suis infection: Biofilm formation

Contact Info

Product

Resources

About