The<i>Acinetobacter</i>trimeric autotransporter adhesin Ata controls key virulence traits of<i>Acinetobacter baumannii</i>

Weidensdorfer, Marko; Ishikawa, Masahito; Hori, Katsutoshi; Linke, Dirk; Djahanschiri, Bardya; Iruegas, Ruben; Ebersberger, Ingo; Riedel-Christ, Sara; Enders, Giulia; Leukert, Laura; Kraiczy, Peter; Rothweiler, Florian; Činátl, Jindřich; Berger, Jürgen; Hipp, Katharina; Kempf, Volkhard A. J.; Göttig, Stephan

doi:10.1080/21505594.2018.1558693

Cited by 54 publications

(62 citation statements)

References 64 publications

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“…It is worth mentioning that ata was present in 78% of the sequenced A. baumannii isolates but only in 3% of the closely related but much less human pathogenic A. calcoaceticus/A. pittii clade [99].…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Vaca

Thibau

Schütz

et al. 2019

Med Microbiol Immunol

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The capacity of pathogenic microorganisms to adhere to host cells and avoid clearance by the host immune system is the initial and most decisive step leading to infections. Bacteria have developed different strategies to attach to diverse host surface structures. One important strategy is the adhesion to extracellular matrix (ECM) proteins (e.g., collagen, fibronectin, laminin) that are highly abundant in connective tissue and basement membranes. Gram-negative bacteria express variable outer membrane proteins (adhesins) to attach to the host and to initiate the process of infection. Understanding the underlying molecular mechanisms of bacterial adhesion is a prerequisite for targeting this interaction by "anti-ligands" to prevent colonization or infection of the host. Future development of such "anti-ligands" (specifically interfering with bacteria-host matrix interactions) might result in the development of a new class of anti-infective drugs for the therapy of infections caused by multidrug-resistant Gram-negative bacteria. This review summarizes our current knowledge about the manifold interactions of adhesins expressed by Gram-negative bacteria with ECM proteins and the use of this information for the generation of novel therapeutic antivirulence strategies.

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Section: Acinetobacter Baumanniimentioning

confidence: 99%

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Vaca

Thibau

Schütz

et al. 2019

Med Microbiol Immunol

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“…Hence, we grew Yersinia at 37°C (Champion et al ., 2009). All Y. ruckeri strains showed a time and dosedependent killing of larvae with a log 10 LD 50 of approximately 10 5 , which is comparable to other important pathogens like Escherichia coli , Klebsiella pneumoniae or Acinetobacter baumannii (Tietgen et al ., 2018; Weidensdorfer et al ., 2019) thereby underlining the pathogenicity of Y. ruckeri . Deletion of yrInv or yrIlm or both reduced virulence by 50% compared to NVH_3758, which indicates that both IATs contribute modestly to virulence without showing a synergy in this infection model.…”

Section: Discussionmentioning

confidence: 99%

The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence

Wróbel

Saragliadis

Pérez-Ortega

et al. 2020

Environmental Microbiology

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Summary Yersinia ruckeri causes enteric redmouth disease (ERM) that mainly affects salmonid fishes and leads to significant economic losses in the aquaculture industry. An increasing number of outbreaks and the lack of effective vaccines against some serotypes necessitates novel measures to control ERM. Importantly, Y. ruckeri survives in the environment for long periods, presumably by forming biofilms. How the pathogen forms biofilms and which molecular factors are involved in this process, remains unclear. Yersinia ruckeri produces two surface‐exposed adhesins, belonging to the inverse autotransporters (IATs), called Y. ruckeri invasin (YrInv) and Y. ruckeri invasin‐like molecule (YrIlm). Here, we investigated whether YrInv and YrIlm play a role in biofilm formation and virulence. Functional assays revealed that YrInv and YrIlm promote biofilm formation on different abiotic substrates. Confocal microscopy revealed that they are involved in microcolony interaction and formation, respectively. The effect of both IATs on biofilm formation correlated with the presence of different biopolymers in the biofilm matrix, including extracellular DNA, RNA and proteins. Moreover, YrInv and YrIlm contributed to virulence in the Galleria mellonella infection model. Taken together, we propose that both IATs are possible targets for the development of novel diagnostic and preventative strategies to control ERM.

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“…In another study, passive immunization with anti-Ata decreased the bacterial count in the lungs of challenged mice. Even though its expression level is variable among A. baumannii strains, this protein is considered as a valuable vaccine candidate because of its high conservancy and importance in bacterial virulence [5,54].…”

Section: Discussionmentioning

confidence: 99%

Protective potentials of a siderophore receptor against Acinetobacter baumannii infections

2019

Biointerface Res Appl Chem

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Acinetobacter baumannii causes nosocomial infections and high mortality rates in the world. Since antibiotic treatment is complicated by extensive drug resistance in A. baumannii strains, other approaches such as vaccination can be a cost-effective solution. Siderophore receptor related to cluster 1 of iron uptake system (WP_000413999) is expressed in early stages of A. baumannii’s infection under iron restricted conditions. In this study, structural characterization and immunogenicity of the target protein in a murine sepsis model were assessed. Structural properties of the siderophore receptor were determined by bioinformatics tools. The gene encoding the antigen was cloned in E. coli BL21(DE3) and was then expressed. The purified recombinant protein was administered to the mice subcutaneously for antibody production. The immunized mice were challenged with the A. baumannii at lethal dose via intraperitoneal injection. Blast results revealed more than 97% identity of the protein in 3472 strains of A. baumannii. This receptor is a TonB-dependent transporter with a barrel domain composed of 22 β-strands connected by external loops and periplasmic turns. Experimental findings showed that the recombinant protein had no toxicity effect on A549 cells. Moreover, the studied protein was able to induce a specific antibody response in the mice. Survival rate of the passive immunized mice was improved against sepsis caused by A. baumannii ATCC 19606 and ABI022 clinical isolate. The study indicated that this siderophore receptor can be considered for further analysis as a potential vaccine target against A. baumannii.

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TheAcinetobactertrimeric autotransporter adhesin Ata controls key virulence traits ofAcinetobacter baumannii

Cited by 54 publications

References 64 publications

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence

Protective potentials of a siderophore receptor against Acinetobacter baumannii infections

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