The <i>ABISMO</i> Mud and Water Sampling ROV for Surveys at 11,000 m Depth

Yoshida, Hiroshi; Ishibashi, Shojiro; Watanabe, Yoshitaka; Inoue, Tomoya; Tahara, Junichiro; Sawa, Takao; Osawa, Haruki

doi:10.4031/mtsj.43.5.31

Cited by 32 publications

(15 citation statements)

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“…1) and the Challenger Deep in the Mariana Trench (11°22′ N, 142°42′ E; 10,332 m water depth) (Fig. 1) were obtained with a gravity corer of the ROV ABISMO (Automatic Bottom Inspection and Sampling Mobile) during the JAMSTEC KR07-17 (December 2007) and KR08-05 (May−June 2008) cruises with the R/V Kairei [42], respectively. The lengths of the cores were 1.0 m and 1.3 m from the Izu-Ogasawara and Mariana Trench, respectively.…”

Section: Methodsmentioning

confidence: 99%

Metagenomic Analysis of Viral Communities in (Hado)Pelagic Sediments

et al. 2013

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In this study, we analyzed viral metagenomes (viromes) in the sedimentary habitats of three geographically and geologically distinct (hado)pelagic environments in the northwest Pacific; the Izu-Ogasawara Trench (water depth = 9,760 m) (OG), the Challenger Deep in the Mariana Trench (10,325 m) (MA), and the forearc basin off the Shimokita Peninsula (1,181 m) (SH). Virus abundance ranged from 106 to 1011 viruses/cm3 of sediments (down to 30 cm below the seafloor [cmbsf]). We recovered viral DNA assemblages (viromes) from the (hado)pelagic sediment samples and obtained a total of 37,458, 39,882, and 70,882 sequence reads by 454 GS FLX Titanium pyrosequencing from the virome libraries of the OG, MA, and SH (hado)pelagic sediments, respectively. Only 24−30% of the sequence reads from each virome library exhibited significant similarities to the sequences deposited in the public nr protein database (E-value <10−3 in BLAST). Among the sequences identified as potential viral genes based on the BLAST search, 95−99% of the sequence reads in each library were related to genes from single-stranded DNA (ssDNA) viral families, including Microviridae, Circoviridae, and Geminiviridae. A relatively high abundance of sequences related to the genetic markers (major capsid protein [VP1] and replication protein [Rep]) of two ssDNA viral groups were also detected in these libraries, thereby revealing a high genotypic diversity of their viruses (833 genotypes for VP1 and 2,551 genotypes for Rep). A majority of the viral genes predicted from each library were classified into three ssDNA viral protein categories: Rep, VP1, and minor capsid protein. The deep-sea sedimentary viromes were distinct from the viromes obtained from the oceanic and fresh waters and marine eukaryotes, and thus, deep-sea sediments harbor novel viromes, including previously unidentified ssDNA viruses.

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Section: Methodsmentioning

confidence: 99%

Metagenomic Analysis of Viral Communities in (Hado)Pelagic Sediments

et al. 2013

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“…The test dive of a novel ROV (remotely operated vehicle) 'ABISMO' was conducted during the cruise JAMSTEC KR07-17 (December 2007) on the R/V Kairei (Yoshida et al, 2009). A sediment core was sampled using a gravity corer equipped with the ROV 'ABISMO' at one of the deepest points of the Ogasawara Trench (29°09′N, 142°49′E, 9760 m) ( Fig.…”

Section: Site Description and Sampling Proceduresmentioning

confidence: 99%

Molecular biological and isotopic biogeochemical prognoses of the nitrification‐driven dynamic microbial nitrogen cycle in hadopelagic sediments

Nunoura

Nishizawa

Kikuchi

et al. 2013

Environmental Microbiology

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There has been much progress in understanding the nitrogen cycle in oceanic waters including the recent identification of ammonia-oxidizing archaea and anaerobic ammonia oxidizing (anammox) bacteria, and in the comprehensive estimation in abundance and activity of these microbial populations. However, compared with the nitrogen cycle in oceanic waters, there are fewer studies concerning the oceanic benthic nitrogen cycle. To further elucidate the dynamic nitrogen cycle in deep-sea sediments, a sediment core obtained from the Ogasawara Trench at a water depth of 9760 m was analysed in this study. The profiles obtained for the pore-water chemistry, and nitrogen and oxygen stable isotopic compositions of pore-water nitrate in the hadopelagic sediments could not be explained by the depth segregation of nitrifiers and nitrate reducers, suggesting the co-occurrence of nitrification and nitrate reduction in the shallowest nitrate reduction zone. The abundance of SSU rRNA and functional genes related to nitrification and denitrification are consistent with the co-occurrence of nitrification and nitrate reduction observed in the geochemical analyses. This study presents the first example of cooperation between aerobic and anaerobic nitrogen metabolism in the deep-sea sedimentary environments.

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“…Four categories of undersea vehicles are now commonly used (NRC, 2004;Kudo, 2008;Tengberg et al, 1995). They are human-occupied vehicles (Kohnen, 2009), remotely operated vehicles (Whitcomb et al, 2003;Murashima et al, 2004;Barry and Hashimoto, 2009;Yoshida et al, 2009), autonomous underwater vehicles (Nakajoh et al, 1998;Bowen et al, 2009;Fletcher et al, 2009) and landers (Tengberg et al, 1995). Currently, JIAO-LONG is the only human-occupied vehicle with a capability to dive more than 6500 m in hadal zones (Cui, 2013).…”

Section: Basic Concepts and Philosophy Of The Movable Laboratory For mentioning

confidence: 97%

“…In addition to marking the deepest point on Earth, Kaiko achieved more than 20 scientific dives to the trenches before being tragically 'lost at sea' in 2003 (Momma et al, 2004). Since the turn of the twenty-first century, two more vehicles have been constructed: the ABISMO vehicle by JAMSTEC (Yoshida et al, 2009) and the American Woods Hole Oceanographic Institution's Nereus vehicle . Unfortunately, Nereus was lost during its six-mile dive to the Kermadec Trench in May 9, 2014.…”

Section: Introductionmentioning

confidence: 99%