The hypoxic brain: Histological and ultrastructural aspects

Reempts, Jos Van

doi:10.1016/0166-4328(84)90177-3

Cited by 61 publications

(25 citation statements)

References 23 publications

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“…These features are apparent in Figs. 3C and 4D and are very similar to those seen in ischemic cell change, or condensed cell change, following in situ ischemia (Brierly, 1976;Van Reempts, 1984). The material between cytoskel-eta1 elements progressively increases in density from in situ-fixed cells through the category I11 cells (Fig.…”

Section: Resultssupporting

confidence: 69%

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N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

Feig¹,

Lipton

1990

Journal of Neurochemistry

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The CA1 pyramidal cells appear damaged in micrographs of guinea pig hippocampal slices incubated in normal physiological buffer at 36-37 degrees C. This is remedied if slices are incubated in modified buffers for the first 45 min. Cell morphology is improved if this buffer is devoid of added Ca2+ and much improved if it contains N-methyl-D-aspartate (NMDA) receptor antagonists or 0 mM Ca2+ and 10 mM Mg2+. The cells then appear similar to CA1 pyramidal cells in situ. These findings support the notion that NMDA receptor activation and Ca2+, acting in the period immediately after slice preparation, permanently damage CA1 pyramidal cells in vitro.

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Section: Resultssupporting

confidence: 69%

“…The cytoplasm of the highly swollen (category IV) cells has a granular appearance with localized collections of organelles (Fig. 5A), similar to that seen in the edematous degeneration following severe anoxia (Van Reempts, 1984;Ikonomidou et al, 1989). As seen at higher power in Fig.…”

Section: Resultssupporting

confidence: 62%

N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

Feig¹,

Lipton

1990

Journal of Neurochemistry

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“…Cell changes, consisting of microvacuolation, appear to be fully reversible in the early post-hypoxic period. Irreversible cell death, coagulative cell change and edematous cell change can be developed in a later phase [55]. High concentrations of oxygen for a period of hours during a specific period of development caused an apoptotic neurodegenerative reaction that deleted large numbers of neurons in the developing brain [56].…”

Section: Discussionmentioning

confidence: 99%

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

et al. 2016

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An air diffusion based system (Airx) was developed to control the dissolved oxygen levels in aquaculture sea cages. The system was introduced and then tested for 37 days in a sea bass sea cage (aerated cage). A second sea bass sea cage, without the AirX, was used as a control. Oxygen levels were measured in both cages at the start of the trial, before the AirX system was introduced, and during the working period of the AirX system. Fish samples were collected 15 days after the AirX system was introduced and at the end of the experiment. Blood smears were prepared and examined microscopically. Erythrocyte major axis, minor axis and area of fish erythrocytes were measured. Leucocyte differentiation was also examined. In the control cage, the fish had significantly larger red blood cells when compared with the red blood cells of the fish in the aerated cage. Histological examination of the gills and brain revealed no morphological differences or alterations between the two groups of fish. This study demonstrated that an air diffuser system could improve the water quality of fish farmed in sea cages and enhance sea bass physiological performance, especially if DO levels fall below 60% oxygen saturation.

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“…1), corresponding to an 8-9 on the scale of Van Reempts [37]. As we have previously reported [11,33], in both HI ?…”

Section: Morphological Studiesmentioning

confidence: 60%

The Cannabinoid WIN 55212-2 Mitigates Apoptosis and Mitochondrial Dysfunction After Hypoxia Ischemia

Alonso-Alconada

Álvarez

et al. 2011

Neurochem Res

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Perinatal hypoxia-ischemia has significant mortality and morbidity due to there is still no specific treatment as a consequence of the complexities of hypoxic-ischemic pathophysiology. The aim of this work was to evaluate the effects of the cannabinoid agonist WIN 55212-2 on apoptotic cell death and mitochondrial dysfunction after perinatal asphyxia in fetal lambs. Animals were assigned to: one SHAM group and two hypoxic-ischemic groups that received a dose of 0.01 μg/kg WIN 55,212-2 (HI + WIN) or not (HI + VEH) after 60 min of partial occlusion of the umbilical cord, and sacrificed 3 h later. Different brain regions were separated for morphological studies, and the same territories were dissociated and analyzed by flow cytometry to quantify apoptosis, to determine mitochondrial integrity and transmembrane potential and to analyze intracellular calcium levels. Our results showed that WIN 55,212-2 reduced apoptotic cell death in all regions studied through the maintenance of mitochondrial integrity and functionality.

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The hypoxic brain: Histological and ultrastructural aspects

Cited by 61 publications

References 23 publications

N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

The Cannabinoid WIN 55212-2 Mitigates Apoptosis and Mitochondrial Dysfunction After Hypoxia Ischemia

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The hypoxic brain: Histological and ultrastructural aspects

Cited by 61 publications

References 23 publications

N‐Methyl‐D‐Aspartate Receptor Activation and Ca2+ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

N‐Methyl‐D‐Aspartate Receptor Activation and Ca2+ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

The Cannabinoid WIN 55212-2 Mitigates Apoptosis and Mitochondrial Dysfunction After Hypoxia Ischemia

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Product

Resources

About

N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices

N‐Methyl‐D‐Aspartate Receptor Activation and Ca²⁺ Account for Poor Pyramidal Cell Structure in Hippocampal Slices