The hypotriglyceridemic effect of biotin supplementation involves increased levels of cGMP and AMPK activation

Aguilera‐Méndez, Asdrúbal; Fernández-Mejía, Cristina

doi:10.1002/biof.1034

Cited by 35 publications

(31 citation statements)

References 40 publications

(67 reference statements)

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“…Among water-soluble vitamins, dietary contents of four vitamins (pantothenic acid, biotin, vitamin B2 and B12) were increased by egg yolk supplementation by 5.40 mg/kg diet, 81.1 mg/kg diet, 600 mg/kg diet and 3.74 mg/kg diet, respectively. However, these increases in water-soluble vitamins were smaller compared with the effective dose on diseases in other reports (42)(43)(44). These estimations indicated that both composition of amino acids and water-soluble vitamins in egg yolk are insufficient for prevention of fatty liver induced by the low-protein diet.…”

Section: Discussionmentioning

confidence: 68%

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Erami

Tanaka

Kawamura

et al. 2016

J Nutr Sci Vitaminol

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Summary Egg yolk is an important source of nutrients and contains different bioactive substances. In the present study, we studied the benefits of egg yolk in preventing low-protein-diet-induced fatty liver in rats. Rats were fed the following diets, which were based on the AIN-76 formula, for 2 wk: an adequate-protein diet containing 20% casein (C), a lowprotein diet containing 5% casein (LP-C), a low-protein diet supplemented with 12.5% egg yolk (LP-EY), and a low-protein diet supplemented with 4.1% egg yolk oil (LP-EYO). The lowprotein diets were adjusted to contain 4.13% protein and 4.7% lipids. The LP-C diet resulted in a greater increase in the liver trigriceride (TG) and the vacuolation and a greater decrease in the serum TG and free fatty acid (FFA) than did the C diet. These deviations in the serum and liver TG, serum FFA levels and the liver histopathology were corrected in rats fed the LP-EY diet but not in those fed the LP-EYO diet. Compared to rats fed the LP-C diet, although the activities of lipogenesis-related enzymes (fatty acid synthase, glucose-6-phosphate dehydrogenase, and malic enzyme) decreased in rats fed both of the LP-EY and LP-EYO diets, the level of the microsomal TG transfer protein (MTP) increased only in rats fed the LP-EY diet. Collectively, these results suggest that dietary egg yolk supplementation decreases the LP diet-induced accumulation of TG in the liver by increasing transport of TG in the liver, and egg yolk oil alone is not sufficient enough to bring about these benefits.

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Section: Discussionmentioning

confidence: 68%

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Erami

Tanaka

Kawamura

et al. 2016

J Nutr Sci Vitaminol

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“…These discrepancies may be explained by the fact that several studies have found that the abundance of selected proteins was not paralleled by the abundance of their mRNAs [7, 43]. In addition, pyruvate carboxylase apoenzyme expression is regulated by biotin at the posttranscriptional level, thereby influencing both protein synthesis and degradation [44].…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation

Ronquillo-Sánchez

Camacho-Carranza

Fernández-Mejía

et al. 2013

BioMed Research International

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Studies have found that biotin favors glucose and lipid metabolism, and medications containing biotin have been developed. Despite the use of biotin as a pharmacological agent, few studies have addressed toxicity aspects including the possible interaction with cytochrome P450 enzyme family. This study analyzed the effects of pharmacological doses of biotin on the expression and activity of the cytochrome P4501A subfamily involved in the metabolism of xenobiotics. Wistar rats were treated daily with biotin (2 mg/kg, i.p.), while the control groups were treated with saline. All of the rats were sacrificed by cervical dislocation after 1, 3, 5, or 7 days of treatment. CYP1A1 and CYP1A2 mRNAs were modified by biotin while enzyme activity and protein concentration were not affected. The lack of an effect of biotin on CYP1A activity was confirmed using other experimental strategies, including (i) cotreatment of the animals with biotin and a known CYP1A inducer; (ii) the addition of biotin to the reaction mixtures for the measurement of CYP1A1 and CYP1A2 activities; and (iii) the use of an S9 mixture that was prepared from control and biotin-treated rats to analyze the activation of benzo[a]pyrene (BaP) into mutagenic metabolites using the Ames test. The results suggest that biotin does not influence the CYP1A-mediated metabolism of xenobiotics.

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“…In recent years it has been considered that supplementation with pharmacological doses of biotin also leads to endocrine, systemic, and metabolic actions [24,[40][41]. To our knowledge, there are no reports exploring the effect of biotin on vastus lateralis muscle carboxylases.…”

Section: Discussionmentioning

confidence: 99%

“…In this tissue, there is truly a constant input and output of tricarboxylic acid cycle intermediaries, which accumulate in metabolic conditions such as diabetes. The lower activity of PC in supplemented mice can explain the hypoglycemic effect that the pharmacological treatment of biotin has in diabetic patients [41,42].…”

Section: Discussionmentioning

confidence: 99%

Differential effect of biotin on carboxylase activity and mice skeletal muscle metabolism

de¹,

Gutiérrez‐Ospina²,

Salcedo³

et al. 2013

ABB

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In mammalian skeletal muscle there are four carboxylases involved in several biochemical processes like gluconeogenesis, tricarboxylic acid cycle anaplerosis, metabolism of fatty acids and metabolism of various amino acids. It has been shown that biotin deficiency reduces body weight at the expense of muscular mass. When necessary, the liver uses skeletal muscle protein to provide glucose and amino acids to organs in need of such compounds. In this paper we analyzed carboxylase specific activities in hind limb skeletal muscle of 3 weeks old BALB/c male mice, at 0, 1, 4, 7, and 14 days of a specific diet with different biotin concentrations. Biotin was used at 0.0, 1.8 or 98.2 mg per kg of food; and was referred to as biotin deficient, sufficient and supplemented, respectively. Water and food supply and consumption by the three groups of mice were the same. Therefore, the observed effects were directly related to biotin ingestion. The body weight of biotin supplemented mice was the same as the body weight of mice in the biotin sufficient group, while biotin deficiency caused body weight reduction after 7 days of biotin depletion. We found that the total protein concentration in the vastus lateralis muscle is associated with the biotin content in the diet. After 7 days, the muscle total protein content was lower in mice of the biotin deficient group while it was higher in the mice from the biotin supplemented group (P < 0.001). Of the four analyzed enzymes, only pyruvate carboxylase specific activity was reduced in both cases: by consuming the supplemented diet and by the lack of this vitamin. Our data show that PC and muscle metabolism are differentially altered by both, biotin excess and biotin deficiency. The mechanisms of these effects are currently under investigation.

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The hypotriglyceridemic effect of biotin supplementation involves increased levels of cGMP and AMPK activation

Cited by 35 publications

References 40 publications

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation

Differential effect of biotin on carboxylase activity and mice skeletal muscle metabolism

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