The hypoosmotic swelling test in fresh rabbit spermatozoa

Amorim, E. A. M.; Torres, Ciro Alexandre Alves; Graham, J. K.; Amorim, L. S.; Santos, Leandro

doi:10.1016/j.anireprosci.2008.02.015

Cited by 26 publications

(14 citation statements)

References 8 publications

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“…These findings greatly differ from the osmolarity of the HSs reported for use in horses (25–100 mOsm/kg; Neild et al. 1999), rabbits (60 mOsm/kg; Amorim et al. 2009), dogs (100 mOsm/kg; Pinto and Kozink 2008) and partially with those described for boars (50–150 mOsm/kg; Vazquez et al.…”

Section: Discussioncontrasting

confidence: 71%

“…In this study, a specific HS for epididymal spermatozoa that has an osmolality of 150 mOsm⁄kg was determined. These findings greatly differ from the osmolarity of the HSs reported for use in horses (25-100 mOsm⁄kg; Neild et al 1999), rabbits (60 mOsm⁄kg; Amorim et al 2009), dogs (100 mOsm⁄kg; Pinto and Kozink 2008) and partially with those described for boars (50-150 mOsm⁄kg;Vazquez et al 1997). Nevertheless, these results are in agreement with HSs used in both bulls (Revell and Mrode 1994) and humans (Jeyendran et al 1984) at 150 mOsm⁄kg.…”

Section: Discussioncontrasting

confidence: 57%

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Hypoosmotic Swelling Test in Alpaca (Vicugna pacos) Epididymal Spermatozoa

Vásquez

Florentini

Valdivia

2012

Reprod Domestic Animals

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The present study intended to develop the hypoosmotic swelling (HOS) test in alpaca for its use in epididymal spermatozoa, to evaluate the membrane functional integrity and determine an appropriate hypoosmotic solution and whether the incubation time of 15 or 60 min is sufficient for the execution of the test. Hypoosmotic solutions (HS) with the following concentrations were used: 50, 100, 150, 200 and 275 mOsm/kg of sodium citrate tribasic dihydrate and d-fructose. Ten microlitres of epididymal sperm sample was mixed in 150 μL of the respective HS and incubated for 15 or 60 min at 38°C. From the proportion of reacted (swollen) spermatozoa, the 150 mOsm/kg HS was the most sensitive (p < 0.05). The exposure times (15 and 60 min) did not have significant differences (p > 0.05) in the proportion of both strong- and total-coiled sperm tails. In conclusion, 150 mOsm/kg HS and 15 min exposure time are optimal to evaluate the plasma membrane functional integrity through the HOS test in alpaca epididymal spermatozoa.

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Section: Discussioncontrasting

confidence: 71%

Section: Discussioncontrasting

confidence: 57%

Hypoosmotic Swelling Test in Alpaca (Vicugna pacos) Epididymal Spermatozoa

Vásquez

Florentini

Valdivia

2012

Reprod Domestic Animals

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“…HOST is a relatively simple test developed for rabbit spermatozoa to evaluate the functional integrity of the spermatozoa membrane [43] against a hypo-osmotic solution of 60 mOsmol/L. The swollen spermatozoa characterized by a coiled tail which is an indication on the sperm intact plasma membrane.…”

Section: Sperm Membrane Integrity: Hypo-osmotic Swelling Test (Host)mentioning

confidence: 99%

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

El-Seadawy

El-Nattat

El-Tohamy

et al. 2017

APJR

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“…In this sense, various studies have been conducted in order to determine the most appropriate osmolality for each different species, as a 60 mOsm/L solution for rabbits (Amorim et al . ) and 150 mOsm/L for humans (Santos et al . ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

et al. 2016

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da Silva, A.M., Bezerra, J.A.B., Campos, L.B., Praxedes, E.C.G., Lima, G.L. and Silva, A.R. 2017. Characterization of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831) recovered by different methods. -Acta Zoologica (Stockholm) 98: 285-291.The aim of the present study was to characterize the epididymal sperm of Spix's yellow-toothed cavy (Galea spixii) through two different recovery methods. Nine sexually mature males were euthanized and the complexes, testes-epididymis, were dissected. For each animal, one epididymis was processed by flotation method and the other was processed by retrograde flushing method, both using a TES-based buffered media. After recovery, we evaluated the sperm for motility, vigour, viability, functional membrane integrity and morphology. Morphometric data from the different sperm regions were evaluated by using an appropriate software. After recovery, both methods provide similar values for all the sperm parameters, aiming the recovery of more than 300 9 10 6 sperm, presenting >50% motile sperm, with normal morphology and functional membrane. The total sperm length in this sperm was 48.87 AE 0.1, and the sperm head presented 9.4%, on average. A notable characteristic was the prominent acrosome found in the G. spixii sperm. In conclusion, we demonstrate that either flotation or retrograde flushing methods are suitable for the recovery of sperm from cauda epididymis of Spix's yellow-toothed cavies.

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The hypoosmotic swelling test in fresh rabbit spermatozoa

Cited by 26 publications

References 8 publications

Hypoosmotic Swelling Test in Alpaca (Vicugna pacos) Epididymal Spermatozoa

Hypoosmotic Swelling Test in Alpaca (Vicugna pacos) Epididymal Spermatozoa

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

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