The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome

Abstract

Background: The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei) RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processingglucosidase II encoding gene are the only known genetic differences in strain RUT C30.

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa

“…One approach to depolymerization of plant cell wall polysaccharides involves using hydrolytic enzymes produced by bacteria and fungi (3). Toward achieving this goal, the filamentous fungus, Hypocrea jecorina (Trichoderma reesei) has been engineered, largely through random mutagenesis and screening, to produce elevated amounts of cellulases (4).…”

Global nutrient profiling by Phenotype MicroArrays: a tool complementing genomic and proteomic studies in conidial fungi

“…The biochemical nature of these observations is unclear, but indicates a dependence of carbon catabolite repression on assimilation of these carbon sources. Seidl et al (2008) also showed that the H. jecorina RUT C30 and its ancestor NG 14 lack an 85-kb genomic fragment, missing additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. They reported that these mutations are not linked to the cre1 locus.…”

“…The latter two carbon sources might reflect enhanced β-glucosidase activity, yet the rest of utilized carbon sources are catabolite repressing compounds and at this point, le Crom et al (2009) stressed that it is not clear whether this is a consequence of the cre1 mutation or it is due to the effect of other affected genes. Previously reported inverse carbon source utilization in correlation with enhanced cellulase production was detected by utilization of α-linked oligosaccharides and glycans (Seidl et al, 2008), and was complemented by the correlation between cellulase production and reduced growth on amino acids. le Crom et al (2009) discussed this phenotype to be related to an ability of the superior cellulase production strains to use a higher portion of their amino acid pool for synthesis of secreted proteins versus growth.…”

“…To determine the utilization of which carbon sources is actually affected by the carbon catabolite repression, Seidl et al (2008) investigated the differences in the growth of a cre1-truncated mutant strain of H. jecorina (RUT C30) (Ilmén et al, 1996) and the respective parent strain NG 14. The results (Fig.…”

“…These two strains were identified on the basis of their superior cellulase activity as well as resistance to 2-deoxyglucose in the presence of glycerol (Seidl et al, 2008). Biolog PMs were used to compare the mutants with their wild-type strain QM 6a.…”