The hydrosmotic effect of vasopressin: A scanning electron-microscope study

Spinelli, F.; Grosso, A.; Sousa, R. C. de

doi:10.1007/bf01870248

Cited by 37 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our observations on the changes in the granular cell mucosal surface agree with Spinelli et al (25), and are at variance with those of Davis et ai. (5).…”

Section: Discussionsupporting

confidence: 93%

“…2), "fused" microvilli forming short rows of anastomosing ridges can be seen on each granular cell. This morphology occurs in tissues bathed with isotonic Na § Ringer's solution on both sides, or with an osmotic gradient of 1:5 or 1:10 from mucosa to serosa, This is in agreement with previous SEM studies of the toad bladder in which the tissues were examined after two different types of preparative techniques (5,25). However, we found that this ridgelike appearance was never uniform throughout the epithelium.…”

Section: Morphologysupporting

confidence: 92%

See 1 more Smart Citation

Mucosal surface morphology of the toad urinary bladder. Scanning electron microscope study of the natriferic and hydro-osmotic response to vasopressin

Mills¹,

Malick²

1978

The Journal of Cell Biology

View full text Add to dashboard Cite

The mucosal cell surface of the toad urinary bladder was examined by scanning electron microscopy, and changes in the structure of the surface of the granular cell were correlated with specific physiological responses to vasopressin. Survey views of the mucosal surface demonstrated that there was no consistent repeating anatomical relationship between the granular cell and the mitochondria-rich cell that would support the concept of cooperativeness in the response to vasopressin. During base-line states of Na+-transport and water flux, the microvilli on the mucosal surface of the granular cell are arranged in a ridge-like network with occasional individual projections. When water flux is increased by exposing the tissue to vasopressin, in the presence of an osmotic gradient across the tissue, the microvilli on the granular cell lose the ridge structure and appear, predominantly, as individual projections. Variability-of this appearance points out the necessity of examining large areas and many samples before the significance of any morphological change can be assessed. Blocking the simultaneously occurring natriferic response of the toad urinary bladder with 10 -2 M ouabain does not prevent these changes in the microvilli. When the hydro-osmotic response is blocked by eliminating the osmotic gradient, the granular cell shows no consistent change in mucosal surface morphology even when fixed at the height of the natriferic response. The mitochondria-rich and mucous cells did not show any change in morphology throughout these studies. We conclude that the changes in the mucosal surface morphology of the toad bladder seen after exposure to vasopressin are a result of the increased water flux that occurs when an osmotic gradient exists across the tissue, and are not related to the natdferic response or any specific alteration in the membrane properties. KEY WORDS toad urinary bladder scanningThe toad urinary bladder has long been used as a electron microscopy vasopressin -granular cell model system for studying the action of the poly- 598J. CELL BIOLOGY 9 The Rockefeller University Press 9

show abstract

“…Our observations on the changes in the granular cell mucosal surface agree with Spinelli et al (25), and are at variance with those of Davis et ai. (5).…”

Section: Discussionsupporting

confidence: 93%

Section: Morphologysupporting

confidence: 92%

Mucosal surface morphology of the toad urinary bladder. Scanning electron microscope study of the natriferic and hydro-osmotic response to vasopressin

Mills¹,

Malick²

1978

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…They were kept in a terrarium and had free access to water (for details see Spinelli, Grosso & De Sousa, 1975).…”

Section: Methodsmentioning

confidence: 99%

Osmotic water flow across the abdominal skin of the toad Bufo marinus: effect of vasopressin and isoprenaline

Sousa

Grosso

1982

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

4. Methohexital and propranolol selectively inhibited the hydrosmotic effects of vasopressin and isoprenaline, respectively, whereas amiloride and ouabain had no effect.5. Mutual inhibition was found between vasopressin and isoprenaline in skins very sensitive to vasopressin. In less sensitive skins isoprenaline further increased Jw despite exposure of the epithelia to supramaximal concentrations of vasopressin. 6. Differential reactivity to vasopressin was found between the skin and the bladder taken from the same toad. In some instances, the bladder responded normally to vasopressin while the skin was totally unresponsive, suggesting the presence of osmoregulatory mechanisms exerting a local modulation of the vasopressin action in different target epithelia of the same animal.

show abstract

“…ADH acts upon the epithelial cells which line the bladder, increasing their luminal membrane permeability to water and small solutes, most notably sodium and urea. It seems clear that ADHstimulated water movement occurs through pore-like pathways [10,12,17,26] which are selective for water alone [21,23,24,29] and restricted to granular-type cells only [6,7,28]. Freeze-fracture electron microscopy reveals that ADH also causes the occurrence of intramembranous particle aggregates in granular cell luminal membrane [3,19].…”

Section: Introductionmentioning

confidence: 99%

Effect of distension on ADH-induced osmotic water flow in toad urinary bladder

Kachadorian

Levine

1982

J. Membrain Biol.

View full text Add to dashboard Cite

We recently described a method by which the resistance to water flow of the luminal membrane of ADH-stimulated toad bladder can be quantitatively distinguished from that of barriers lying in series with it. This method requires estimated of both total bladder water permeability (assessed by transbladder osmotic water flow at constant gradient) and luminal membrane water permeability (assessed by quantitation of the frequency of ADH-induced luminal membrane particle aggregates). In the present study we examined the effect of bladder distension on transepithelial osmotic water flow before and during maximal ADH stimulation. Base-line water flow was unaffected by bladder distension, but hormonally stimulated flow increased systematically as bladders became more distended. Distension had no effect on the frequency of ADH-induced intramembranous particle aggregates. By comparing the relationships between aggregate frequency and hormonally induced water permeability in distended and undistended bladders, we found that distension appeared to enhance ADH-stimulated water flow by decreasing the resistance of the series permeability barrier while the apparent water permeability associated with each single luminal membrane aggregate was unaffected. In that bladder distension caused tissue thinning, the series resistance limiting ADH-stimulated water flow appears to be accounted for by deformable barriers within the bladder tissue itself, probably unstirred layers of water.

show abstract

The hydrosmotic effect of vasopressin: A scanning electron-microscope study

Cited by 37 publications

References 19 publications

Mucosal surface morphology of the toad urinary bladder. Scanning electron microscope study of the natriferic and hydro-osmotic response to vasopressin

Mucosal surface morphology of the toad urinary bladder. Scanning electron microscope study of the natriferic and hydro-osmotic response to vasopressin

Osmotic water flow across the abdominal skin of the toad Bufo marinus: effect of vasopressin and isoprenaline

Effect of distension on ADH-induced osmotic water flow in toad urinary bladder

Contact Info

Product

Resources

About