The hydrolysis of phosphorylcholine-containing metabolites in plant tissues: partial purification of a CDP-choline hydrolase from Solanum tuberosum

Moreau, Robert A.; Asmann, Paul T.

doi:10.1016/0168-9452(91)90005-s

Plant Science

1991

DOI: 10.1016/0168-9452(91)90005-s

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The hydrolysis of phosphorylcholine-containing metabolites in plant tissues: partial purification of a CDP-choline hydrolase from Solanum tuberosum

Robert A. Moreau

Paul T. Asmann

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Cited by 2 publications

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“…Several chromatographic methods have been developed for separation and quantification of plant, animal, and microbial lipids. , Among these, hydrophilic interaction liquid chromatography (HILIC) columns have been recently introduced for metabolomic profiling and lipid profiling . This type of column is based on normal phase chromatography, where a polar stationary phase is used together with an associated layer of water that promotes chromatographic separation. , …”

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Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

et al. 2013

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We here present simple and rapid methods for fast screening of yeast lipids in Saccharomyces cerevisiae. First we introduced a microwave-assisted technique for fast lipid extraction that allows the extraction of lipids within 10 min. The new method enhances extraction rate by 27 times, while maintaining product yields comparable to conventional methods (n = 14, P > 0.05). The recovery (n = 3) from spiking of synthetic standards were 92 ± 6% for cholesterol, 95 ± 4% for triacylglycerol, and 92 ± 4% for free fatty acids. Additionally, the new extraction method combines cell disruption and extraction in one step, and the approach, therefore, not only greatly simplifies sample handling but also reduces analysis time and minimizes sample loss during sample preparation. Second, we developed a chromatographic separation that allowed separation of neutral and polar lipids from the extracted samples within a single run. The separation was performed based on a three gradient solvent system combined with hydrophilic interaction liquid chromatography-HPLC followed by detection using a charged aerosol detector. The method was shown to be highly reproducible in terms of retention time of the analytes (intraday; 0.002-0.034% RSD; n = 10, interday; 0.04-1.35% RSD; n = 5) and peak area (intraday; 0.63-6% RSD; n = 10, interday; 4-12% RSD; n = 5).

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confidence: 99%

Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

et al. 2013

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Modulation of CTP: Phosphocholine cytidylyltransferase activity in choline-sensitive and choline-resistant tobacco cells

et al. 1993

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The hydrolysis of phosphorylcholine-containing metabolites in plant tissues: partial purification of a CDP-choline hydrolase from Solanum tuberosum

Cited by 2 publications

References 12 publications

Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

Modulation of CTP: Phosphocholine cytidylyltransferase activity in choline-sensitive and choline-resistant tobacco cells

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