The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)

Xing, Liping; Chen, Qian; Cao, Aizhong; Li, Yingbo; Jiang, Zhengning; Li, Minghao; Jin, Xiahong; Hu, Jingjing; Zhang, Yiping; Wang, Xiue; Chen, Peidu

doi:10.1371/journal.pone.0072571

Cited by 23 publications

(23 citation statements)

References 47 publications

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“…Similar to the observations made in a previous study 15 , transiently overexpressed GFP-fused SRC2-1 was exclusively localized to the plasma membrane in N. benthamiana leaf epidermis cells, whereas SGT1 was localized throughout the cells including in the plasma membrane, cytoplasm, and nucleus in the present study. Similar observations of SGT1 were also previously reported in other plant species 53 . Given that no NLS is found in SGT1, its nuclear targeting is possibly due to the interaction with other nuclear-targeting proteins.…”

Section: Discussionsupporting

confidence: 90%

SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1

Liu

Shi

et al. 2016

Sci Rep

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PcINF1 was previously found to induce pepper defense response by interacting with SRC2-1, but the underlying mechanism remains uninvestigated. Herein, we describe the involvement of SGT1 in the PcINF1/SRC2-1-induced immunity. SGT1 was observed to be up-regulated by Phytophthora capsici inoculation and synergistically transient overexpression of PcINF1/SRC2-1 in pepper plants. SGT1-silencing compromised HR cell death, blocked H2O2 accumulation, and downregulated HR-associated and hormones-dependent marker genes’ expression triggered by PcINF1/SRC2-1 co-overexpression. The interaction between SRC2-1 and SGT1 was found by the yeast two hybrid system and was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation analyses. The SGT1/SRC2-1 interaction was enhanced by transient overexpression of PcINF1 and Phytophthora capsici inoculation, and SGT1-silencing attenuated PcINF1/SRC2-1 interaction. Additionally, by modulating subcellular localizations of SRC2-1, SGT1, and the interacting complex of SGT1/SRC2-1, it was revealed that exclusive nuclear targeting of the SGT1/SRC2-1 complex blocks immunity triggered by formation of SGT1/SRC2-1, and a translocation of the SGT1/SRC2-1 complex from the plasma membrane and cytoplasm to the nuclei upon the inoculation of P. capsici. Our data demonstrate that the SGT1/SRC2-1 interaction, and its nucleocytoplasmic partitioning, is involved in pepper’s immunity against P. capsici, thus providing a molecular link between Ca2+ signaling associated SRC2-1 and SGT1-mediated defense signaling.

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Section: Discussionsupporting

confidence: 90%

SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1

Liu

Shi

et al. 2016

Sci Rep

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“…Forty‐eight hours post‐inoculation, the accumulation of H 2 O 2 in plant cells was detected by staining with DAB (Sigma‐Aldrich, http://www.sigmaaldrich.com/) as described previously (Xing et al ., ). Leaves were soaked in 1 mg ml −1 DAB solution (pH 3.8) for 16 h at 25°C in the dark, and de‐colored in boiling 95% ethanol for 10 min.…”

Section: Methodsmentioning

confidence: 97%

“…Callose deposition in the infected leaves was detected using aniline blue diammonium salt (AB; Sigma‐Aldrich). Before microscopic examination, leaves at 48 h post‐inoculation were first de‐colored using 95% ethanol and then stained in 0.01% aniline blue solution dissolved in 150 m m K 2 HPO 4 (pH 9.5) for 8 h (Xing et al ., ; Wu et al ., ). Trypan blue staining was also used to observe the mycelium structures at 7 dpi.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

et al. 2017

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Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). enhanced disease resistance1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show constitutively activated defense responses. This makes EDR1 an ideal target for approaches using new genome-editing tools to improve resistance to powdery mildew. We cloned TaEDR1 from hexaploid wheat and found high similarity among the three homoeologs of EDR1. Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew, indicating that TaEDR1 negatively regulates powdery mildew resistance in wheat. We used CRISPR/Cas9 technology to generate Taedr1 wheat plants by simultaneous modification of the three homoeologs of wheat EDR1. No off-target mutations were detected in the Taedr1 mutant plants. The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death. Our study represents the successful generation of a potentially valuable trait using genome-editing technology in wheat and provides germplasm for disease resistance breeding.

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“…tritici compared to wild-type. 48 The transcript abundance of TaPAL increased by 3.4-fold in shoots at 72 h after MeJA treatment ( Fig. 2; Fig.…”

Section: Other Important Plant Defense Genesmentioning

confidence: 95%

Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

Liu

Carvalhais

Kazan

2016

Plant Signaling & Behavior

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The jasmonic acid (JA) signaling pathway plays key roles in a diverse array of plant development, reproduction, and responses to biotic and abiotic stresses. Most of our understanding of the JA signaling pathway derives from the dicot model plant Arabidopsis thaliana, while corresponding knowledge in wheat is somewhat limited. In this study, the expression of 41 genes implicated in the JA signaling pathway has been assessed on 10 day-old bread wheat seedlings, 24 h, 48 h, and 72 h after methyljasmonate (MeJA) treatment using quantitative real-time PCR. The examined genes have been previously reported to be involved in JA biosynthesis and catabolism, JA perception and signaling, and pathogen defense in wheat shoots and roots. This study provides evidence to suggest that the effect of MeJA treatment is more prominent in shoots than roots of wheat seedlings, and substantial regulation of the JA pathway-dependent defense genes occurs at 72 h after MeJA treatment. Results show that the expression of 22 genes was significantly affected by MeJA treatment in wheat shoots. However, only PR1.1 and PR3 were significantly differentially expressed in wheat roots, both at 24 h post-MeJA treatment, with other genes showing large variation in their gene expression in roots. While providing marker genes on JA signaling in wheat, future work may focus on elucidating the regulatory function of JA-modulated transcription factors, some of which have well-studied potential orthologs in Arabidopsis.

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The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.)

Cited by 23 publications

References 47 publications

SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1

SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

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