The Husbandry and Welfare of Non‐Traditional Laboratory Rodents

Sherwin, CM

doi:10.1002/9781444318777.ch25

Cited by 6 publications

(2 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So far, no effects of forceps handling on aggressive behavior have been reported in the literature, consequently no previous data exist for us to compare our results. However, aggression is a welfare issue for mice and moreover creates additional variability [3, 37]. Interestingly the fecal corticosterone levels, which are thought to reflect stress experienced by the mice, did not show any difference between handling groups.…”

Section: Resultsmentioning

confidence: 99%

Effect of three different forms of handling on the variation of aggression-associated parameters in individually and group-housed male C57BL/6NCrl mice

et al. 2019

View full text Add to dashboard Cite

Mice are social animals hence group-housing of mice is preferred over individual housing. However, aggression in group-housed male mice under laboratory housing conditions is a well-known problem leading to serious health issues, including injury or death. Therefore, group-housed mice are frequently separated for welfare reasons. In this study, we investigated the effect of 3 different handling methods (tail, forceps, tube) in 2 different housing conditions (single vs. group) on the variance of aggression-associated parameters in male C57BL/6NCrl mice over 8 weeks. Blood glucose concentration, body weight, body temperature, plus number and severity of bite wounds and barbering intensity in group-housed mice were recorded. An assessment of nest complexity was also performed weekly. Feces were collected in week 3 and 7 for analysis of corticosterone metabolites. We also monitored the level of aggression by recording the behavior of group-housed animals after weekly cage cleaning. An open field test followed by a social novel object test, a light/dark box test, a hotplate and a resident-intruder test were performed at the end of the 8-week handling period. Post-mortem, we assessed organ weights. We found that forceps-handled mice, independent of the housing condition, had significantly higher levels of stress-induced-hyperthermia and enhanced aggression after cage cleaning, and they performed worse in the nest complexity test. In addition, handling male mice by the tail seems to be most effective to reduce aggressiveness after transferring animals into new cages, thereby representing an appropriate refinement.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of three different forms of handling on the variation of aggression-associated parameters in individually and group-housed male C57BL/6NCrl mice

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Cannon and Nedergaard, 2004;Legendre and Davesne, 2020). Some of them, the homeotherms, maintain their organs and nervous system at a nearly constant temperature (within ± 2 °C) by regulating their thermogenesis and thermolysis (Scholander, 1955) while poikilotherms possess deep body temperature which covaries with environmental temperatures (Schmidt-Nielsen et al, 1966;Bennett et al, 1993;Sherwin, 2010;Allali et al, 2013;Nicol, 2017). Maintaining a high and constant temperature throughout the body in non-normothermic conditions is extremely energy-consuming for homeotherms.…”

Section: Introductionmentioning

confidence: 99%

Intraskeletal variability in phosphate oxygen isotope composition reveals regional heterothermies in marine vertebrates

Séon¹,

Amiot

Suan

et al. 2022

Preprint

View full text Add to dashboard Cite

Abstract. Strategies used by marine vertebrates to regulate their body temperature can result in local variations, and the knowledge of these regional heterothermies is crucial for better understanding the thermophysiologies of extant and extinct organisms. In order to investigate regional heterothermy in vertebrates, we analysed the oxygen isotope composition of phosphatic skeletal elements (δ18Op) of two poikilothermic (Atlantic bluefin tuna and swordfish) and three homeothermic endotherms (dolphins). We observed a consistent link between δ18Op variations and temperature heterogeneities recorded by classical methods. Our δ18Op data indicate that: (i) bone hydroxyapatite of the axial skeleton of dolphins mineralize at a warmer temperature than that of the appendicular one, (ii) the skull is the warmest body region in swordfish, and (iii) Atlantic bluefin tuna possesses high body temperature in the skull and visceral mass region. These results demonstrate the possibility of tracking regional heterothermies in extant marine vertebrates using the δ18Op, paving the way to direct assessment of thermophysiological specificities of both living and extinct vertebrates. From a paleoenvironmental perspective, the significant observed δ18Op variability questions the use of some taxa or random skeletal elements for the reconstruction of paleoceanographic parameters such as seawater temperature and δ18O.

show abstract

Other Disease Vectors and Their Control

Bisen

Raghuvanshi

2013

Emerging Epidemics

View full text Add to dashboard Cite

The Husbandry and Welfare of Non‐Traditional Laboratory Rodents

Cited by 6 publications

References 111 publications

Effect of three different forms of handling on the variation of aggression-associated parameters in individually and group-housed male C57BL/6NCrl mice

Effect of three different forms of handling on the variation of aggression-associated parameters in individually and group-housed male C57BL/6NCrl mice

Intraskeletal variability in phosphate oxygen isotope composition reveals regional heterothermies in marine vertebrates

Other Disease Vectors and Their Control

Contact Info

Product

Resources

About