The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture

Abstract

Fetal bovine serum (FBS) is a common supplement to in vitro culture media. A workshop was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and to discuss serum replacement methods. When bovine fetuses are exposed after slaughter of the dam, they can suffer only if they inflate their lungs with air and increase their blood oxygen to levels compatible with awareness. Preventing fetuses from breathing air or killing them by an efficient method, according to clearly defined safeguards, ensures that fetal blood collection is humane. Since serum is a supplement of unknown composition, which could be contaminated with unwanted factors, there are scientific and safety reasons for omitting FBS from culture media. Several media have been developed in which minimal or no animal derived components are present. Also, different cell types have been adapted to serum-free media. As yet, no standard serum free media are present, and each cell type requires its own medium composition. Among other recommendations, the establishment of a public database with information on cell types and their serum-free medium composition is proposed.

Evaluation of Methylcellulose and Dimethyl Sulfoxide as the Cryoprotectants in a Serum-Free Freezing Media for Cryopreservation of Adipose-Derived Adult Stem Cells

“…Serum is routinely added to the cryopreservation media as a source of nutrients, and other undefi ned factors, in spite of scientifi c and technical disadvantages to its inclusion, its high cost, and the increasing availability of serum-free alternatives [17][18][19][20]. Obviously, for in vivo clinical use, the elimination of animal serum proteins and all possible sources of infectious diseases such as hepatitis, human immunodefi ciency virus (HIV), and bovine spongiform encephalopathy (BSE) is a prerequisite [21][22][23]. Therefore, it is necessary to replace serum as a supplement in freezing media used for cryopreserving ASCs.…”

Good Cell Culture Practice for stem cells and stem-cell-derived models

“…In some cases, it is possible to use fully chemically defined media with appropriate hormones and growth factors (van der Valk et al, 2004). A compilation of commercially available serum-free media was published recently 5 (Brunner et al, 2010).…”

Expressão gênica empregando pseudopartículas em células de mamíferos (HEK 293T e Huh 7.0) cultivadas em diferentes meios de cultura livres de soro.

“…A célula HEK 293, é uma linhagem derivada de células embrionárias humanas de rim, transformada com adenovírus tipo 5 humano, foi desenvolvida há 37 anos (GRAHAM et al, 1977), mas apenas nos últimos anos tem sido extensivamente usada, sendo a linhagem humana provavelmente mais utilizada para pesquisas relacionadas a expressão de proteínas VAN DER VALK et al, 2004).…”

“…Os esforços para desenvolver um substituto são principalmente baseados em duas intenções. Por um lado, a coleta e a produção do SFB são dois fatores eticamente problemáticos, por isso uma substituição é necessária (BRUNNER et al, 2010;JOCHEMS et al, 2002;VAN DER VALK et al, 2004). Por outro lado, os campos de rápida expansão de célula in vitro, cultura de tecidos, engenharia biomédica e a terapia celular requerem definidas e seguras condições de cultura celular livre de componentes de origem animal (ATALA, 2007;VAN DER VALK et al, 2010).…”

“…Esta linhagem exibe uma morfologia epitelial e uma das razões para sua larga aplicação é o fato destas células crescerem facilmente em suspensão e em SFM disponíveis comercialmente (DUROCHER; BUTLER, 2009;LIU et al, 2006;MEI et al, 2006;ZHU, 2012 VAN DER VALK et al, 2004).…”