The human type‐C retrovirus, HTLV, in blacks from the Caribbean region, and relationship to adult T‐cell leukemia/lymphoma

Blattner, William A.; Kalyanaraman, V. S.; Robert-Guroff, Marjorie; Lister, T. Andrew; Galton, D. J.; Sarin, Prem S.; Crawford, Michael H.; Catovsky, Daniel; Greaves, Melvyn F.; Gallo, Robert C.

doi:10.1002/ijc.2910300302

Cited by 537 publications

(139 citation statements)

References 23 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…The observations that anti-pl9 + thymic epithelium is HTLV p24 negative, that no HTLV proviral DNA sequences can be detected with HTLV c-DNA probes by Southern blot hybridization techniques, and that all thymus tissues tested from donors older than 8 wk gestation were anti-pl9 + provide strong evidence against this. Moreover, epidemiologic data strongly suggest that HTLV-associated Tcell leukemia is horizontally passed as an infectious disease and not by vertical transmission (4)(5)(6)(7)(8)(9). Molecular hybridization studies have verified this and proven that the infection is postzygotic (3,21).…”

Section: Discussionmentioning

confidence: 94%

Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny.

Haynes¹,

Robert-Guroff²,

Metzgar³

et al. 1983

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Using monoclonal antibody 12/1-2 against a 19,000-dalton human T cell leukemia virus (HTLV) protein (anti-p19), previously demonstrated to be reactive with HTLV-infected human cells, but not in numerous other uninfected cells, we found a reactive antigen to be expressed on the neuroendocrine component of human thymic epithelial cells but not on any other normal epithelial or neuroendocrine human tissues. Moreover, this reactive antigen is acquired on neuroendocrine thymic epithelium during thymic ontogeny--first appearing on fetal thymic epithelial cells between 8 and 15 wk gestation. While only a portion of thymic epithelial cells in the subcapsular cortical region of 15- and 24-wk fetal thymuses contained anti-p19+ epithelial cells, the entire subcapsular cortical region of newborn thymus epithelium was anti-p19+. By age 3 yr, normal subjects' entire subcapsular cortical and medullary thymic epithelium was anti-p19+. Using antibody against HTLV core protein, p24, and c-DNA probes for HTLV DNA, neither HTLV-specific p24 protein nor proviral DNA could be demonstrated in anti-p19+ thymic epithelial tissue. However, thymic epithelial extracts, disrupted HTLV extracts, as well as purified HTLV p19 antigen all inhibited the binding of anti-p19 antibody to thymic epithelium. Thus, anti-p19 may recognize a determinant on an HTLV-encoded 19,000-dalton structural protein that is shared by human thymic epithelium. Alternatively, anti-p19 defines a host encoded protein that is selectively expressed by normal thymic epithelium, and is induced to be expressed in HTLV-infected malignant T cells.

show abstract

Section: Discussionmentioning

confidence: 94%

Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny.

Haynes¹,

Robert-Guroff²,

Metzgar³

et al. 1983

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…The number of syncytia decreased gradually with increase in cell passages. Thus, most of these syn- (5,10,13,35,36). Therefore, we next examined whether syncytia formation was neutralized by these antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…There seems to be slight heterogeneity even among ATL viruses isolated in Japan-that is, analyses of nucleotide sequences of ATL viruses integrated in MT-1 cells (7) and in lymphocytes of an ATL patient (8) showed the presence of several base changes in the long terminal repeats of these two viruses. Patients having ATL are clustered in southwestern Japan and the West Indies (1,4,9,10). HTLV has also been detected in patients from different parts of the world (11).…”

mentioning

confidence: 99%

Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay.

Hoshino¹,

Shimoyama²,

Misawa³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

119

View full text Add to dashboard Cite

Recently 10 T-cell lines were established from patients with adult T-cell leukemia (ATL). During establishment of these cell lines, it was found that when T-cell lines expressing the ATL-associated retroviral antigen were cocultivated with 8C cat cells, multinucleated syncytia were formed. Retroviral antigen-negative T-cell lines did not induce syncytia. Peripheral blood lymphocytes obtained from ATL patients did not express the retroviral antigen before cultivation in vitro but became positive for the retroviral antigen after cultivation for a short period; these retroviral antigen-positive lymphocytes, but not retroviral antigen-negative lymphocytes, induced syncytia upon cocultivation with 8C cells. Peripheral blood lymphocytes isolated from patients with chronic lymphocytic leukemia of T-cell origin or Skzary syndrome or from normal adults and lymph node cells from a patient with immunoblastic lymphadenopathy-like T-cell lymphoma did not express the retroviral antigen even after cultivation in vitro and did not induce syncytia upon cocultivation with SC cells. Thus, there was complete agreement between the presence of the retroviral antigen in established T-cell lines or freshly isolated peripheral blood lymphocytes and their ability to induce syncytia. Syncytia formation was enhanced 5-to 20-fold by the presence of Polybrene and inhibited by addition of plasma of ATL patients to the cocultures. Syncytia were detected within 4 hr on cocultivation of 8C cells with the retroviral antigen-positive T-cells, indicating that most syncytia were formed by early polykaryocytosis. After cocultivation, a clone of 8C cells that harbored the AU virus genome and had syncytia-inducing activity was isolated. These findings indicate that the retrovirus associated with AU has syncytiainducing activity. Syncytia induction assay using 8C cells will be useful for detection and characterization of human retrovirus associated with T-cell malignancies.

show abstract

“…Infection with HTLV-I is endemic in parts of Japan, South America, Africa, and the Caribbean (Hinuma et al, 1981Blattner et al, 1982;Saxinger et al, 1984;Slattery et al, 1999). HTLV-I carriers do not develop disease until 20-30 years after infection, and the cumulative lifetime risk of a carrier developing ATL is approximately 4% (Murphy et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Cellular Transformation by the HTLV-I Tax Protein, a Jack-of-All-Trades

2003

View full text Add to dashboard Cite

The human type‐C retrovirus, HTLV, in blacks from the Caribbean region, and relationship to adult T‐cell leukemia/lymphoma

Cited by 537 publications

References 23 publications

Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny.

Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny.

Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay.

Cellular Transformation by the HTLV-I Tax Protein, a Jack-of-All-Trades

Contact Info

Product

Resources

About