The Human Thymus Contains a Novel Population of B Lymphocytes

Isaacson, Peter G.; Norton, A. J.; Addis, B. J.

doi:10.1016/s0140-6736(87)92622-5

Cited by 206 publications

(136 citation statements)

References 13 publications

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“…Whether CD23 is expressed intrathymically remains to be established. A low number of CD23 + B lymphocytes have been observed in human thymus (14). As CD23 is expressed by other cells, it remains possible that thymic accessory cells, including epithelial cells, express CD23.…”

Section: Resultsmentioning

confidence: 99%

Soluble CD23 (Fc epsilon RII) and interleukin 1 synergistically induce early human thymocyte maturation.

Mossalayi

Lecron

Dalloul

et al. 1990

The Journal of Experimental Medicine

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During development, lymphoid stem cells migrate into the thymic rudiment where they differentiate into functionally mature T lymphocytes (1). Among human thymocytes, CD7 + CD2 -CD3 -CD4 -CD8 -(referred hereafter as CD7') cells represent the earliest identifiable step (2-4) . In vitro, these precursors were able to acquire mature T cell antigens (3, 4), but cellular interactions and cytokines necessary for this process are poorly understood. We have previously reported that enriched populations of B lymphocytes from blood produce factors (3, 5) that promote in vitro development of blood-and bone marrow-derived CD7 + CD2 -precursors. Comparing the biochemical characteristics of this B cell-derived activity (3) to known B cell-derived molecules, we found its striking homology to soluble CD23 (sCD23 ; sFcERII) (6, 7) . Using recombinant sCD23 (rsCD23) (7), we then assayed the effect of this molecule alone or with rIL-1 and/or rIL-2 on purified CD7 + thymic precursors . Our results provide direct evidence that sCD23 and rIL-1 synergistically induce CD7 + prothymocytes maturation into CD2'CD3'TCRa//3+ CD4 + and/or CD8 + cells that respond to CD2 triggering and rIL-2.

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Section: Resultsmentioning

confidence: 99%

Soluble CD23 (Fc epsilon RII) and interleukin 1 synergistically induce early human thymocyte maturation.

Mossalayi

Lecron

Dalloul

et al. 1990

The Journal of Experimental Medicine

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“…2,9 As a normal counterpart, asteroid B-cells have been described in the normal thymic medulla. 10,11 It is intriguing that MedB-1 cells preferentially adhere to normal thymic medulla even after 10 years of growing in suspension.…”

Section: Discussionmentioning

confidence: 99%

“…2,8,9 As a normal counterpart to this neoplasm, a peculiar population of thymic medullary B-cells has been proposed. 10,11 Histologically, mediastinal B-cell lymphoma varies in cell size from medium to large and is, although inconsistently, associated with a sclerosing reaction. 12 The forthcoming World Health Organization Classification of Neoplastic Diseases of the Hematopoietic and Lymphoid Tissues currently lists mediastinal (thymic) B-cell lymphoma as a variant of diffuse large cell lymphoma 13 as it was formerly designated in the REAL classification.…”

mentioning

confidence: 99%

MedB-1, a human tumor cell line derived from a primary mediastinal large B-cell lymphoma

et al. 2001

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Primary mediastinal B-cell lymphoma is a locally highly aggressive but poorly disseminating tumor composed of medium sized or large cells most probably of thymic medullary origin. It has a mature B-cell phenotype, typically lacks immunoglobulin expression and has variable defects in expression of HLA-molecules. We present here a cell line, MedB-1, derived from such a tumor. As is frequently found in mediastinal B-cell lymphomas in situ, MedB-1 is CD10 ؊ , CD19 ؉ ,

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“…Cells expressing typical B cell markers IgM, CD19, CD20, and CD22 can be found in the normal human fetal, postnatal, and adult thymus (6,7). Human thymic B cells exhibit an activated phenotype and accumulate within the medulla, particularly around Hassall's corpuscles, but also in the perivascular and intralobular spaces (6,8).…”

mentioning

confidence: 99%

“…Human thymic B cells exhibit an activated phenotype and accumulate within the medulla, particularly around Hassall's corpuscles, but also in the perivascular and intralobular spaces (6,8). Thymic B cells comprise a minor, but discernible, population in mice, appearing in the thymus during early fetal development and reaching a stable frequency by birth, therein maintained throughout adult life (9)(10)(11).…”

mentioning

confidence: 99%

A Role for Intrathymic B Cells in the Generation of Natural Regulatory T Cells

Walters

Webster

Daley

et al. 2014

The Journal of Immunology

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B cells inhabit the normal human thymus, suggesting a role in T cell selection. In this study, we report that B cells can modulate thymic production of CD4+ Foxp3+ T cells (regulatory T cells [Tregs]). Mice with transgenic expression of BAFF (BAFF-Tg) harbor increased numbers of Helios+Foxp3+ thymic Tregs and, similar to some human autoimmune conditions, also exhibit increased numbers of B cells colonizing the thymus. Distinct intrathymic B cell subpopulations were identified, namely B220+, IgM+, CD23hi, CD21int cells; B220+, IgM+, CD23lo, CD21lo cells; and a population of B220+, IgM+, CD23lo, CD21hi cells. Anatomically, CD19+ B cells accumulated in the thymic medulla region juxtaposed to Foxp3+ T cells. These intrathymic B cells engender Tregs. Indeed, thymic Treg development was diminished in both B cell–deficient BAFF-Tg chimeras, but also B cell–deficient wild-type chimeras. B cell Ag capture and presentation are critical in vivo events for Treg development. In the absence of B cell surface MHC class II expression, thymic expansion of BAFF-Tg Tregs was lost. Further to this, expansion of Tregs did not occur in BAFF-Tg/Ig hen egg lysozyme BCR chimeras, demonstrating a requirement for Ag specificity. Thus, we present a mechanism whereby intrathymic B cells, through the provision of cognate help, contribute to the shaping of the Treg repertoire.

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The Human Thymus Contains a Novel Population of B Lymphocytes

Cited by 206 publications

References 13 publications

Soluble CD23 (Fc epsilon RII) and interleukin 1 synergistically induce early human thymocyte maturation.

Soluble CD23 (Fc epsilon RII) and interleukin 1 synergistically induce early human thymocyte maturation.

MedB-1, a human tumor cell line derived from a primary mediastinal large B-cell lymphoma

A Role for Intrathymic B Cells in the Generation of Natural Regulatory T Cells

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