The human thymic dendritic cell phenotype and its modification in culture

Lafontaine, Marielle; Landry, Diane; Montplaisir, S

doi:10.1016/0008-8749(92)90286-x

Cited by 28 publications

(11 citation statements)

References 25 publications

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“…Only a few reports have dealt with the purification of thymic DCs in humans, and it has always been assumed that thymic DCs were a homogeneous population (23)(24)(25). In this study, we identified three distinct CD4 + Lin -DC populations in human thymus: (a) HLA-DR int CD11c -pDC, representing the most abundant population of thymic DCs; (b) HLA-DR int CD11c + imDCs; and (c) HLA-DR hi CD11c + mDC/IDCs.…”

Section: Discussionmentioning

confidence: 99%

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Human thymus contains IFN-α–producing CD11c–, myeloid CD11c+, and mature interdigitating dendritic cells

Bendriss‐Vermare¹,

Barthélemy²,

Durand³

et al. 2001

J. Clin. Invest.

179

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Section: Discussionmentioning

confidence: 99%

“…Initial studies have characterized human thymic DCs as a homogeneous MHC class II + population that differs from its mouse counterpart in being mainly CD4 + 8 - (23)(24)(25)(26). However, recent studies reported that thymic medulla contains both mDCs and IL-3Rα + pDCs (15,27,28).…”

mentioning

confidence: 99%

Human thymus contains IFN-α–producing CD11c–, myeloid CD11c+, and mature interdigitating dendritic cells

Bendriss‐Vermare¹,

Barthélemy²,

Durand³

et al. 2001

J. Clin. Invest.

179

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“…Studies of mature DCs in humans ex vivo [31][32][33][34][35][36] have been hampered by the difficulty of isolating these cells. Often, shortterm culture steps were required to overcome this problem.…”

Section: Introductionmentioning

confidence: 99%

Human thymus contains 2 distinct dendritic cell populations

Vandenabeele

Hochrein

Mavaddat

et al. 2001

Blood

129

119

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In this study, 2 distinct populations of mature dendritic cells (DCs) were identified in the human thymus. The major population is CD11b ؊ , CD11c ؉ , and CD45RO low and does not express myeloidrelated markers. It displays all the characteristics of mature DCs with a typical dendritic morphology, high surface levels of HLA-DR, CD40, CD83, and CD86, and expression of DC-lysosome-associated membrane glycoprotein messenger RNA (mRNA). In addition, CD11b ؊ thymic DCs do not express macrophage inflammatory protein-1␣ (MIP-1␣) mRNA, but express thymus-expressed chemokine (TECK) mRNA and are able to secrete bioactive interleukin 12 (IL-12) upon stimulation. In contrast, the minor and variable thymic DC population is CD11b ؉ , CD11c high , and CD45RO high and comprises CD83 ؉ CD14 ؊ mature and CD83 ؊ CD14 ؉ immature DCs. It expresses macrophage-colony stimulating factor receptor, MIP-1␣ mRNA and high amounts of decysin mRNA after CD40 activation, but does not express TECK and is a weak bioactive IL-12 producer. Also identified were the IL-3R␣ high plasmacytoid cells, which are present in the thymic cortex and medulla. Upon culture with IL-3, granulocyte/macrophage-colony stimulating factor, and CD40 ligand, the plasmacytoid cells can adopt a phenotype resembling that of freshly isolated CD11b ؊ thymic DCs. However, these plasmacytoid-derived DCs fail to secrete bioactive IL-12; therefore, conclusions cannot be made about a direct relation between thymic plasmacytoid cells and CD11b ؊ DCs. Whereas CD11b ؉ thymic DCs appear to be related to tonsillar germinal-center DCs, the major CD11b ؊ IL-12-secreting human thymus DC population has similarities to mouse CD11b ؊ CD8 ؉ DCs. IntroductionDendritic cells (DCs) are professional antigen presenting cells 1 that form a dynamic network throughout most tissues and organs and that are crucial for the immune surveillance of the body. [2][3][4] The current model is that Langerhans cells, immature DCs found in epithelia, are the precursors of mature "interdigitating" DCs, found in the T-cell zones of lymphoid organs. 5 Immature interstitial DCs in various tissues, such as the dermis, move into germinal centers as germinal center DCs (GCDCs). In the lymph nodes, tonsils, and spleen, the mature DCs present the antigen captured in the periphery to naive T cells and induce immunity. The DCs of the thymus have a somewhat different role, namely, to present selfantigens and induce negative selection of potential auto-reactive T-cell clones. 6 Until recently, the interdigitating DCs were considered a single population of mature DCs. However, this laboratory and others showed that distinct DC subsets of different lineage derivation and different functions 7-13 could be distinguished in mouse lymphoid organs. On the one hand, CD8 ϩ DCs, lacking myeloid markers such as CD11b and apparently arising from a lymphoid-committed progenitor, 14 are typically potent interleukin 12 (IL-12)-secreting mature DCs. 10,13,15 CD8 ϩ DCs are present at various levels in all mouse lymphoid organs and constitute...

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“…Because of this, comprehensive and detailed knowledge about the maturation pathways of different subsets of DCs at distinct locations is required. Until now, human DCs have been identified and characterized in peripheral blood (PB), 7 lung, 8 thymus, 9 dermis, 10 tonsils, 11 spleen, 12 and lymph nodes 13 but, to our knowledge, there is little information about the characteristics of bone marrow (BM) DC precursors (pre‐DCs) 14,15 . Previous studies have shown that different subpopulations of DCs (e.g., myeloid DCs, plasmacytoid DCs, monocyte‐derived DCs, and Langerhans cells) originate from CD34+ progenitor cells in the BM, but the ontogeny and specific differentiation pathways of each DC subset still remain largely unknown 16 .…”

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confidence: 99%