The Human “Prion-like” Protein Doppel Is Expressed in Both Sertoli Cells and Spermatozoa

Peoc’h, Katell; Serres, Catherine; Frobert, Yveline; Martin, Caroline P.; Lehmann, Sylvain; Chasseigneaux, Stéphanie; Sazdovitch, Véronique; Grassi, Jacques; Jouannet, Pierre; Launay, Jean‐Marie; Laplanche, Jean‐Louis

doi:10.1074/jbc.m206357200

Cited by 69 publications

(83 citation statements)

References 37 publications

(42 reference statements)

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“…PrP C has also been found on the raft domain from the ovine sperm membrane (Gatti et al 2002, Ecroyd et al 2004), a result previously observed for cattle and human sperm (Shaked et al 1999, Peoc'h et al 2002. However, we were unable to detect PrP Res after proteinase K treatment of scrapie-infected ram seminal plasma (Gatti et al 2002), suggesting the absence of PrP Sc and probably the absence of infectivity, which, however, remained to be proved.…”

Section: Introductioncontrasting

confidence: 45%

Semen from scrapie-infected rams does not transmit prion infection to transgenic mice

Sarradin¹,

Melo²,

Barc³

et al. 2008

Reproduction

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Scrapie is the most common transmissible spongiform encephalopathy (TSE) in livestock. Natural contamination in sheep flocks is presumed to occur by maternal transmission to offspring. However, horizontal prion transmission from animal to animal exists and may be significant in sustaining and spreading contagion in the field. Artificial insemination is widely used in modern farming, and as large amounts of prion protein have been found in sheep sperm membrane, epididymal fluid and seminal plasma, horizontal transmission by this route was hypothesized since no clear information has been obtained on possible sexual transmission of TSE. We therefore tested the contamination levels of semen from scrapie-infected rams at different stages of incubation, including the clinical phase of the disease. We report here that under our experimental conditions ram semen did not transmit infectivity to scrapie-susceptible transgenic mice overexpressing the V 136 R 154 Q 171 allele of the sheep prion (PRNP) gene.These results suggest that artificial insemination and natural mating have a very low or negligible potential for the transmission of scrapie in sheep flocks.Reproduction (2008) 135 415-418

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Section: Introductioncontrasting

confidence: 45%

Semen from scrapie-infected rams does not transmit prion infection to transgenic mice

Sarradin¹,

Melo²,

Barc³

et al. 2008

Reproduction

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show abstract

“…Moreover, the mutant sperm exhibited abnormal morphologies and impaired acrosome function. Consistently, it has been reported that PrPLP/Dpl is expressed in spermatids in mice and spermatozoa and Sertoli cells in humans (23,24). These results indicate that PrPLP/ Dpl is involved in spermatogenesis.…”

Section: Normal Functions Of Prplp/dplsupporting

confidence: 73%

Molecular biology of prion protein and its first homologous protein

Sakaguchi

2007

J. Med. Invest.

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“…Dpl expression induces neurological signs only in PrP knockout mice [1,5,7,8] and except for transient expression in the brain of neonatal mice [9] Prnd mRNA and/or Dpl protein are not expressed in the central nervous system (CNS) of healthy animals. On the other hand, intense expression has been recorded in both testis and heart [1,7,10]. Furthermore, the localization of human Dpl on Sertoli cells and spermatozoa [10] and the lack of expression observed in cases of male sterility in mice [11] suggests that the main function of this protein is male fertility regulation.…”

Section: Introductionmentioning

confidence: 99%

Identification of bovine doppel protein in testis, ovary and ejaculated spermatozoa

et al. 2005

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Doppel (Dpl) protein is a recently identified prion-like protein. Although Dpl might be expressed in the brain after prion gene deletion, in both human and mice Dpl is normally expressed only in testis and spermatozoa, where it appears to be involved in male fertility. Little information is available so far about the expression pattern of Dpl in bovines, thus, hampering possible research on the role of this protein in bovine infertility. We have thus, designed, produced and validated through Western blotting a polyclonal antibody against bovine Dpl. With this antibody we then screened bovine tissues for Dpl expression by immunohistochemistry. Ejaculated spermatozoa were screened by flow cytometry and immunocytochemistry.Bovine Dpl was expressed in all the developing stages of germinal cells, from spermatogones to ejaculated spermatozoa, in Sertoli cells and in ovarian follicles (granulosa cells and follicular fluid). Dpl immunoreactivity was also found on other tissues, where endothelial cells, peripheral nerves and scattered lymphocytes stained positive. This distribution pattern suggests that Dpl might be involved in sperm maturation/capacitation in bovines, like it might be in mice. This hypothesis needs to be verified by widespread application of the flow cytometric protocol established in this paper on spermatozoa from animals with reduced fertility.

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The Human “Prion-like” Protein Doppel Is Expressed in Both Sertoli Cells and Spermatozoa

Cited by 69 publications

References 37 publications

Semen from scrapie-infected rams does not transmit prion infection to transgenic mice

Semen from scrapie-infected rams does not transmit prion infection to transgenic mice

Molecular biology of prion protein and its first homologous protein

Identification of bovine doppel protein in testis, ovary and ejaculated spermatozoa

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