The Human PC346 Xenograft and Cell Line Panel: A Model System for Prostate Cancer Progression

Disconnecting the Yin and Yang Relation of Epidermal Growth Factor Receptor (EGFR)-Mediated Delivery: A Fully Synthetic, EGFR-Targeted Gene Transfer System Avoiding Receptor Activation

“…Both xenograft and cell line are androgen responsive, secrete prostate-specific antigen (PSA), and harbor the wild-type androgen receptor (van Weerden et al, 1996;Marques et al, 2006). In addition, PC346 xenograft and cell line express EGFR (Supplementary Fig.…”

“…Human PC346C cells (van Weerden et al, 1996;Marques et al, 2006) were cultured in DMEM-F12 medium (Cambrex BioWhittaker, Verviers, Belgium) supplemented with 2% FBS (PAN Biotech, Aidenbach, Germany), 1% insulintransferrin-selenium (GIBCO-BRL/Invitrogen), 0.01% bovine serum albumin (Boehringer Mannheim, Mannheim, Germany), epidermal growth factor (10 ng/ml; SigmaAldrich), penicillin-streptomycin antibiotics (penicillin, 100 U/ml; streptomycin, 100 mg/ml; Cambrex BioWhittaker) plus the following additions: fibronectin (100 ng/ml; Harbor Bio-Products, Tebu-bio, Boechout, Belgium), fetuin (20 ng/ ml; ICN Biomedicals, Zoetermeer, The Netherlands), cholera toxin (50 ng/ml), 0.1 mM phosphoethanolamine, triiodothyronine (0.6 ng/ml), dexamethasone (500 ng/ml) (all from Sigma-Aldrich), and 0.1 nM 17-methyltrienolone (R1881; New England Nuclear/PerkinElmer Life Sciences, Boston MA). Cells were grown in T25 Primaria tissue culture flasks (BD Biosciences, Benelux, The Netherlands) at 37°C under a 5% CO 2 humidified atmosphere.…”

Human xenograft models as useful tools to assess the potential of novel therapeutics in prostate cancer

“…This success has triggered other groups, primarily in the United States, to intensify their efforts resulting in a further extension of human-derived PC xenografts such as the CWR, MDA Pca, LuCaP and LAPC series of xenografts established by different groups in the United States (Table 1). All these xenograft models are made available to other researchers (Bosland et al, 1996, Navone et al, 1999.The development of permanent cell lines from PC xenografts has been rather complicated, being successful only for the PC346 xenograft, resulting in the PC346C cell line (Marques et al, 2006). In recent years, a small number of xenograft-derived cell lines have been established by other research groups as well.…”

“…Also, new efforts are directed towards specific blocking of enzymes involved in (intratumoural) steroidogenesis as studies have indicated that HRPC may have the potential capacity to produce its own androgens (Montgomery et al, 2008). The PC346 xenograft system (Marques et al, 2006) as well as some of the CWR22, LuCAP and LAPC xenograft lines show progressive, androgen-independent growth after androgen ablation of tumourbearing mice. These resistant, androgen-independent sublines are valuable assets to study androgen-resistant molecular pathways (Hendriksen et al, 2006).…”

“…These novel cell lines PC346C, 22Rv1, CWR-R1, DuCaP, LAPC-4, MDA Pca1, MDA Pca 2a, MDA Pca 2b and VCaP have all been karyotyped and compared in an extensive study by van Bokhoven et al (2003). In Rotterdam, further in vivo and in vitro selection procedures have resulted in additional PC346 sublines with different (molecular) characteristics that may be related to the various stages of PC progression (Marques et al, 2005(Marques et al, , 2006. The present set of xenograft models and cell lines represent the various disease stages of human PC and strongly add to the options for preclinical testing.…”