The human myocardial two‐dimensional gel protein database: Update 1994

Corbett, Joseph M.; Wheeler, Colin; Baker, Cathy S.; Yacoub, Magdi H.; Dünn, Michael J.

doi:10.1002/elps.11501501209

Cited by 67 publications

(34 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Silver-stained gels were scanned using a Biorad GS-710 calibrated imaging densitometer and analytical images were analysed using PDQuest v6.2.1 (Biorad) (Krauss et al, 1990;Corbett et al, 1994). After detection of spots, the gels were aligned, landmarked and matched.…”

Section: Densitometry and Gel Image Analysismentioning

confidence: 99%

Androgens target prohibitin to regulate proliferation of prostate cancer cells

et al. 2004

View full text Add to dashboard Cite

Proteins involved in the growth response of prostate cancer cells to androgen were investigated by comparing the proteomes of LNCaP cells treated with vehicle or androgen. Whole-cell lysates were separated by twodimensional PAGE, and HPLC-MS/MS was used to identify androgen-regulated proteins. Prohibitin, a protein with cell-cycle regulatory activity, was shown to be downregulated by 50% following androgen stimulation. Western blot and reverse transcription-PCR experiments confirmed the result and showed that regulation occurs at the level of transcription. To determine the importance of prohibitin in androgen-stimulated growth, we used transient transfection to overexpress the protein and RNA interference to knock down the protein. Subsequent FACS analysis showed that cells with reduced levels of prohibitin showed a slight but reproducible increase in the percentage of population in cell cycle, while cells with increased prohibitin levels showed a clear reduction in the percentage entering cell cycle, following dihydrotestosterone stimulation, when compared to untransfected controls. Confocal microscopy showed localization of prohibitin in the nucleus as well as the mitochondria of LNCaP cells. It therefore seems that the regulation of prohibitin is a vital part of the cellular growth response to androgen stimulation in LNCaPs and prohibitin may have a nuclear regulatory role in cell-cycle progression.

show abstract

Section: Densitometry and Gel Image Analysismentioning

confidence: 99%

Androgens target prohibitin to regulate proliferation of prostate cancer cells

et al. 2004

View full text Add to dashboard Cite

show abstract

“…This database [35], maintained at the Heart Science Centre of Harefield Hospital, contains proteins identified on 2-DE of the human heart [36]. The individual entries may be accessed either through a clickable image (Rule 4) or by selection from the complete list of entries.…”

Section: Human Heart 2-d Gel Protein Databasementioning

confidence: 99%

Federated two‐dimensional electrophoresis database: A simple means of publishing two‐dimensional electrophoresis data

et al. 1996

View full text Add to dashboard Cite

While a two-dimensional electrophoresis (2-DE) database is a relatively old concept, in recent years it generated renewed interest within the 2-DE community due to two main factors: (i) The high reproducibility of the current 2-DE method allows 2-DE images to be exchanged and compared between laboratories. (ii) The recent development of faster and more powerful techniques for protein identification such as microsequencing, matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) and amino acid composition makes the production of reference protein maps and 2-DE databases cost- and time-effective. Additionally, the Internet network's current increase in popularity, combined with the rapid growth of Internet-connected laboratories, provides a straightforward means of publishing and sharing 2-DE data. While a small number of laboratories have already successfully published their data over the net, the increasing number of 2-DE database servers that are currently being set up will sooner or later require some kind of standardization. Unfortunately, standardization can be a long and cumbersome process inevitably leading to undesirable compromises. A federated database offers a simple and efficient way to publish and share 2-DE data without the need for standardization. Taking advantage of Internet protocols such as World Wide Web, they allow each laboratory to maintain their own database and to interconnect it with other similar databases through the use of active cross-references. This paper first presents guidelines for building a federated 2-DE database that may easily be followed by most laboratories. It then briefly reviews the state-of-the-art in networked 2-DE databases, and finally describes the SWISS-2DPAGE database which fully implements the concept of a federated 2-DE database.

show abstract

“…Very detailed databases of 2-D protein separations have also been established with different determinations for human body fluids (PDD, Lemkin et al, 1995), human liver (Hughes et al, 1993), plasma and red blood cells (Golaz et al, 1993), human heart (Corbett et al, 1994) and Saccharomyces cerevisiae (YPD, Garrels et al, 1995 …”

Section: Introductionmentioning

confidence: 99%

Identification of vegetative proteins for a two-dimensional protein index of Bacillus subtilis

et al. 1997

View full text Add to dashboard Cite

Twenty-three of the most prominent spots which are visible on twodimensional (2-D) protein gels of Bacillus subtilis crude extracts were selected as marker spots for the construction of a 2-D protein index. N-terminal sequencing of the corresponding proteins resulted in the identification of enzymes involved in glycolysis, TCA cycle, pentose phosphate cycle, amino acid metabolism, nucleotide biosynthesis and translation. Using computer analysis of the 2-D protein gels, most of these metabolic enzymes were found to be synthesized at a reduced rate after different stresses and glucose starvation. Such an approach permits a rapid and global evaluation of the regulation of different branches of metabolism in response to various physiological conditions.

show abstract

The human myocardial two‐dimensional gel protein database: Update 1994

Cited by 67 publications

References 22 publications

Androgens target prohibitin to regulate proliferation of prostate cancer cells

Androgens target prohibitin to regulate proliferation of prostate cancer cells

Federated two‐dimensional electrophoresis database: A simple means of publishing two‐dimensional electrophoresis data

Identification of vegetative proteins for a two-dimensional protein index of Bacillus subtilis

Contact Info

Product

Resources

About