The Human Milk Oligosaccharide 2′-Fucosyllactose Quenches Campylobacter jejuni–Induced Inflammation in Human Epithelial Cells HEp-2 and HT-29 and in Mouse Intestinal Mucosa

Yu, Zhuoteng; Nanthakumar, N. Nanda; Newburg, David S.

doi:10.3945/jn.116.230706

Cited by 105 publications

(90 citation statements)

References 46 publications

(56 reference statements)

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“…Fucose metabolism in Campylobacter strains isolated from GEMS. Despite previous research suggesting that breastfeeding protects against C. jejuni-induced diarrhea (11,28), reports from studies such as GEMS and MAL-ED indicated that a large proportion of breastfed children with diarrhea were Campylobacter positive, and our results unexpectedly showed even higher proportions of this genus in breastfed infants than in nonbreastfed infants. C. jejuni and C. coli were isolated in the original GEMS using selective plates for these two species (25).…”

contrasting

confidence: 99%

“…In LMICs, diarrheal diseases are the second leading cause of mortality in children under 5 years of age, and infection with Campylobacter is one of the major causes of gastroenteritis in these children (1,2,7,34). Previous studies suggest that breastfeeding protects infants from Campylobacter-induced diarrhea by reducing the ingestion of contaminated water/foods and lessening contact with unsanitary surroundings through closer interactions with the mother (7,12,28). In addition, breastfeeding increases exposure to fucosylated HMOs, particularly 2=-fucosyllactose in secretor mothers, which is predicted to act as a binding decoy to prevent C. jejuni colonization of the intestine (11).…”

Section: Discussionmentioning

confidence: 99%

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Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Bian

Garber

Cooper

et al. 2020

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Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asia, we examined fecal microbial compositions, bacterial isolates, and their carbohydrate metabolic pathways in Campylobacter-positive infants <1 year of age from the Global Enterics Multicenter Study. Exclusively breastfed infants with diarrhea exhibited high Campylobacter abundances, and this negatively correlated with bacterial carbohydrate metabolism. Although C. jejuni and Campylobacter coli are prevalent among these infants, the second most abundant Campylobacter species was a new species, which we named “Candidatus Campylobacter infans.” Asymptomatic Campylobacter carriers also possess significantly different proportions of specific gut microbes compared to diarrheal cases. These findings provide insight into Campylobacter infections in infants in sub-Saharan Africa and South Asia and help inform strategies aimed at eliminating campylobacteriosis in these areas. IMPORTANCE Campylobacter is the primary cause of bacterial diarrhea in the United States and can lead to the development of the postinfectious autoimmune neuropathy known as Guillain-Barré syndrome. Also, drug-resistant campylobacters are becoming a serious concern both locally and abroad. In low- and middle-income countries (LMICs), infection with Campylobacter is linked to high rates of morbidity, growth stunting, and mortality in children, and breastfeeding is important for infant nutrition, development, and protection against infectious diseases. In this study, we examined the relationship between breastfeeding and Campylobacter infection and demonstrate the increased selection for C. jejuni and C. coli strains unable to metabolize fucose. We also identify a new Campylobacter species coinfecting these infants with a high prevalence in five of the seven countries in sub-Saharan Africa and South Asia examined. These findings indicate that more detailed studies are needed in LMICs to understand the Campylobacter infection process in order to devise a strategy for eliminating this pathogenic microbe.

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contrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Bian

Garber

Cooper

et al. 2020

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“…A second study conducted by the same group confirmed that fucosylated human milk oligosaccharides inhibit campylobacter colonization of human intestinal mucosa ex vivo [43]. Yu et al tested the ability of 2'-FL to inhibit C. jejuni infection of the intestinal epithelium and C. jejuni-associated mucosal inflammation [44]. In an in vitro model, 2'-FL attenuated 80% of C. jejuni invasion (p <0.05) and decreased the release of [44].…”

Section: For Instance Ruiz-palacios Et Al Demonstrated That Human MImentioning

confidence: 96%

“…Yu et al tested the ability of 2'-FL to inhibit C. jejuni infection of the intestinal epithelium and C. jejuni-associated mucosal inflammation [44]. In an in vitro model, 2'-FL attenuated 80% of C. jejuni invasion (p <0.05) and decreased the release of [44].…”

Section: For Instance Ruiz-palacios Et Al Demonstrated That Human MImentioning

confidence: 99%

Human Milk Oligosaccharides: 2’-fucosyllactose (2’-FL) and Lacto-N-neotetraose (LNnT) in Infant Formula

Vandenplas¹,

Berger²,

Carnielli³

et al. 2018

Preprint

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IntroductionPreprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:The authors reviewed the published evidence on the presence of oligosaccharides in human milk (HMO) and their benefits in in vitro and in vivo studies, as well as to evaluate published data on the addition of some of HMOs to infant formula. . MethodPubMed was searched from inception to April 2018 ResultsThe presence of HMOs is a dynamic process, determined by many factors such as duration of lactation, environmental and genetic factors. HMOs may support immune function development and provide protection against infectious diseases directly through the interaction of the gut epithelial cells or indirectly through the modulation of the gut microbiota, including the stimulation of the bifidobacteria. Only a few of the many HMOs can be made industrially, and are added to infant formula. They have been shown to be safe and well tolerated, including normal growth and a trend towards health benefit. ConclusionsHMOs are one of the major differences between cow's milk and human milk , and evidence indicates that these components do have a health promoting benefit. The addition of one or two of these components to infant formula is safe, and brings infant formula closer to human milk.

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“…La 2´FL bloqueó in vitro, la invasión del tubo digestivo por Campylobacter así como la liberación de péptidos indicadores de daño por enterocitos Hep-2 y HT-29 de origen humano, a semejanza de lo observado en los ratones. Otros efectos observados en los ratones incluían menores pérdidas de peso y lesiones histológicas menos intensas 43 .…”

Section: Los Hmos Y La Microbiota Intestinal De Los Lactantesunclassified

Leche Materna: Características funcionales de los oligosacáridos de la leche materna (Parte 2)

Tesarschü

2019

Rev. chil. nutr.

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The Human Milk Oligosaccharide 2′-Fucosyllactose Quenches Campylobacter jejuni–Induced Inflammation in Human Epithelial Cells HEp-2 and HT-29 and in Mouse Intestinal Mucosa

Abstract: Background: Campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. Binding of virulent C. jejuni

Cited by 105 publications

References 46 publications

Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Human Milk Oligosaccharides: 2’-fucosyllactose (2’-FL) and Lacto-N-neotetraose (LNnT) in Infant Formula

Leche Materna: Características funcionales de los oligosacáridos de la leche materna (Parte 2)

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The Human Milk Oligosaccharide 2′-Fucosyllactose Quenches Campylobacter jejuni–Induced Inflammation in Human Epithelial Cells HEp-2 and HT-29 and in Mouse Intestinal Mucosa

Abstract: Background: Campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. Binding of virulent C. jejuni

Cited by 105 publications

References 46 publications

Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Campylobacter Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study

Human Milk Oligosaccharides: 2&rsquo;-fucosyllactose (2&rsquo;-FL) and Lacto-N-neotetraose (LNnT) in Infant Formula

Leche Materna: Características funcionales de los oligosacáridos de la leche materna (Parte 2)

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Product

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Human Milk Oligosaccharides: 2’-fucosyllactose (2’-FL) and Lacto-N-neotetraose (LNnT) in Infant Formula