The HUman MicroNucleus project on eXfoLiated buccal cells (HUMNXL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

Bonassi, Stefano; Coşkun, Erdem; Ceppi, Marcello; Lando, Cecilia; Bolognesi, Claudia; Burgaz, Sema; Holland, Nina; Kirsh-Volders, Micheline; Knasmueller, Siegfried; Zeiger, Errol; Carnesoltas, Deyanira; Cavallo, Delia; Silva, Juliana da; Andrade, Vanessa Moraes de; Demircigil, Gonca Cakmak; Odio, Aníbal Domínguez; Dönmez-Altuntaş, Hamiyet; Gattás, Gilka Jorge Fígaro; Giri, Ashok Kumar; Giri, Sarbani; Gómez‐Meda, Belinda Claudia; Gómez‐Arroyo, Sandra; Hadjidekova, Valeria; Haverić, Anja; Kamboj, Mala; Kurteshi, Kemajl; Martino-Roth, M G; Montoya, Regina Montero; Nersesyan, Armen; Pastor-Benito, Susana; Salvadori, Daisy Maria Fávero; Shaposhnikova, Alina; Stopper, Helga; Thomas, Philip; Torres-Bugarı́n, Olivia; Yadav, Abhay Singh; González, Guillermo Zúñiga; Fenech, Michael

doi:10.1016/j.mrrev.2011.06.005

Cited by 323 publications

(266 citation statements)

References 31 publications

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“…These numbers were previously determined in a multicenter study with 30 laboratories worldwide, which analyzed 5000 individuals for formation of MN and other nuclear anomalies. 16 Our MN levels at baseline were close to the lower value of the mean worldwide frequency found by Bonassi et al 16 In the present study, 1000 cells/individual were counted, although the literature suggests using a minimum of 2000 cells/individual. 17,18 The study evaluated the genotoxicity of a local agent, as is the case of the effect of bleaching gels on the cells of the gingival epithelium (stratified parakeratinized squamous epithelium).…”

Section: Discussionsupporting

confidence: 87%

“…In contrast, the age factor is well characterized by a steady increase in MN cells according to advancing age. 16 Since the average age of the volunteers was 27.62 ± 7.05 years, their age did not influence the outcome. The participants in this study were recruited from a well-controlled, randomized clinical trial.…”

Section: Discussionmentioning

confidence: 99%

“…Another advantage of this minimally invasive approach is that it can be used without establishing cell cultures. 16 The spontaneous frequency of MN in human buccal exfoliated cells is between 0.3% and 1.7%. These numbers were previously determined in a multicenter study with 30 laboratories worldwide, which analyzed 5000 individuals for formation of MN and other nuclear anomalies.…”

Section: Discussionmentioning

confidence: 99%

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Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching

et al. 2015

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Dental bleaching has become one of the most frequently requested esthetic treatments in dental offices. Despite the high clinical success observed with this procedure, some adverse effects have been reported, including a potential for developing premalignant lesions, root resorption and tooth sensitivity, especially when misused. The aim of this study was to evaluate the genotoxic response using a micronucleus (MN) assay, after the application of two concentrations of carbamide peroxide. Thirty-seven patients were divided into two groups and randomly received either a 10% carbamide peroxide (CP) (19) or a 16% carbamide peroxide (18) concentration for 21 days in individual dental trays. Gingival margin cells were collected immediately before the first use (baseline), and then 15 and 45 days after baseline. The cells were placed on a histological slide, stained by the Feulgen technique, and evaluated by an experienced blinded examiner. One thousand cells per slide were counted, and the MN rate was determined. The two groups were analyzed by the Wilcoxon rank-sum test and the Kruskal-Wallis equality-of-populations rank test. A slight increase in MN was observed for both groups, in comparison with the baseline, at 15 days. However, no difference was observed between the two groups (10% and 16%), at either 15 or 45 days (p = 0.90). When bleaching is not prolonged or not performed very frequently, bleaching agents containing carbamide peroxide alone will not cause mutagenic stress on gingival epithelial cells.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching

et al. 2015

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“…Diet, disease status (e.g. presence of inflammatory diseases), seasonal variation, and physical stress are all important confounding factors that influence an individual's background level for any parameter under consideration (Moller 2005;Battershill et al 2008;Bonassi et al 2011;Fenech et al 2011;Tenorio et al 2013;Collins et al 2014). There is evidence that different factors may have different impact depending on the specific genotoxic endpoints (e.g.…”

Section: Human Versus Non-human Test Resultsmentioning

confidence: 99%

Genotoxicity Expert Panel review: weight of evidence evaluation of the genotoxicity of glyphosate, glyphosate-based formulations, and aminomethylphosphonic acid

Brusick¹,

Aardema²,

Kier

et al. 2016

Critical Reviews in Toxicology

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published a monograph concluding there was strong evidence for genotoxicity of glyphosate and glyphosate formulations and moderate evidence for genotoxicity of the metabolite aminomethylphosphonic acid (AMPA). These conclusions contradicted earlier extensive reviews supporting the lack of genotoxicity of glyphosate and glyphosate formulations. The IARC Monograph concluded there was strong evidence of induction of oxidative stress by glyphosate, glyphosate formulations, and AMPA. The Expert Panel reviewed the genotoxicity and oxidative stress data considered in the IARC Monograph, together with other available data not considered by IARC. The Expert Panel defined and used a weight of evidence (WoE) approach that included ranking of studies and endpoints by the strength of their linkage to events associated with carcinogenic mechanisms. Importantly, the Expert Panel concluded that there was sufficient information available from a very large number of regulatory genotoxicity studies that should have been considered by IARC. The WoE approach, the inclusion of all relevant regulatory studies, and some differences in interpretation of individual studies led to significantly different conclusions by the Expert Panel compared with the IARC Monograph. The Expert Panel concluded that glyphosate, glyphosate formulations, and AMPA do not pose a genotoxic hazard and the data do not support the IARC Monograph genotoxicity evaluation. With respect to carcinogenicity classification and mechanism, the Expert Panel concluded that evidence relating to an oxidative stress mechanism of carcinogenicity was largely unconvincing and that the data profiles were not consistent with the characteristics of genotoxic carcinogens. ARTICLE HISTORY

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“…Over the past decades, biomarker-based approaches have been applied in the assessment of exposure to genotoxic agents and increases of these biomarkers are considered early events associated with disease-related changes [28]. For surrogate biomarkers to have disease predictability, it must be demonstrated that the measured genotoxic events really mimic disease-causing genotoxic events [25].…”

Section: Biomarkers Of Genotoxicitymentioning

confidence: 99%

Human Biomonitoring – An overview on biomarkers and their application in Occupational and Environmental Health

Ladeira¹,

Viegas²

2016

Biomonitoring

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Human biomonitoring (HBM) is a scientificallydeveloped approach for assessing human exposures to natural and synthetic compounds from environment, occupation, and lifestyle. It relies on the measurement of particular substances or biological breakdown products, known as metabolites, in human tissues and/or fluids, and also includes the study of their effects and the possible influence of individual susceptibility as response modulators. HBM is a growing area of knowledge used for exposure and risk assessment in environmental and occupational health, and its importance has been increasing as a result of advancements in the ability to measure greater numbers of chemicals in the human body and tissues. In order to achieve this purpose, HBM focuses on the use of biomarkers as measurable indicators of early changes in biological systems. However, because data interpretation requires caution, it is strongly recommended that the interpretation of HBM results be combined with air monitoring data or pharmacokinetic modelling in order to better understand exposure sources and the metabolism of chemicals.

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The HUman MicroNucleus project on eXfoLiated buccal cells (HUMNXL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

Cited by 323 publications

References 31 publications

Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching

Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching

Genotoxicity Expert Panel review: weight of evidence evaluation of the genotoxicity of glyphosate, glyphosate-based formulations, and aminomethylphosphonic acid

Human Biomonitoring – An overview on biomarkers and their application in Occupational and Environmental Health

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