The human liver matrisome – Proteomic analysis of native and fibrotic human liver extracellular matrices for organ engineering approaches

Daneshgar, Assal; Klein, Oliver; Nebrich, Grit; Weinhart, Marie; Tang, Peter; Arnold, Alexander; Ullah, Imran; Pohl, J.; Moosburner, Simon; Raschzok, Nathanael; Strücker, Benjamin; Bahra, Marcus; Pratschke, Johann; Sauer, Igor M.; Hillebrandt, Karl H.

doi:10.1016/j.biomaterials.2020.120247

Cited by 21 publications

(31 citation statements)

References 44 publications

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“…Furthermore, due to the destructive nature of the solubilization methods and the sampling of organs and tissues, proteomics does not provide information regarding specific regions and distribution of ECM proteins. Different preparation protocols for the creation of decellularized ECM-scaffolds diversely effect matrisome compositions, as described by a recent paper published by Daneshgar and colleagues (30). In this study, it was observed that the human liver matrisome constitutes of many relatively lower abundant matrisome-associated proteins, and that different preparation protocols influenced more the matrisome-associated fraction than the overall core matrisome.…”

Section: Discussionmentioning

confidence: 51%

“…For example, fibrotic stage-specific changes in ECM composition during chronic HCV infection correlates with progressive accumulation of collagens I and II, followed by elastin over expression (28). Similarly, Daneshgar et al reported upregulation of MMPs 23B and 28 and versican, in decellularized human liver samples with increasing grades of fibrosis and cirrhosis (30). Aetiology specific differences have also been observed.…”

Section: Ecm In Chronic Liver Diseasementioning

confidence: 97%

“…Over 150 ECM proteins have been identified in the human liver (9,30). In this review we highlight 20 liver ECM proteins, selected for their implication in CLD and that have been shown to modulate immune responses.…”

Section: Immunomodulatory Properties Of the Ecmmentioning

confidence: 99%

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Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

et al. 2020

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Chronic liver disease when accompanied by underlying fibrosis, is characterized by an accumulation of extracellular matrix (ECM) proteins and chronic inflammation. Although traditionally considered as a passive and largely architectural structure, the ECM is now being recognized as a source of potent damage-associated molecular pattern (DAMP)s with immune-active peptides and domains. In parallel, the ECM anchors a range of cytokines, chemokines and growth factors, all of which are capable of modulating immune responses. A growing body of evidence shows that ECM proteins themselves are capable of modulating immunity either directly via ligation with immune cell receptors including integrins and TLRs, or indirectly through release of immunoactive molecules such as cytokines which are stored within the ECM structure. Notably, ECM deposition and remodeling during injury and fibrosis can result in release or formation of ECM-DAMPs within the tissue, which can promote local inflammatory immune response and chemotactic immune cell recruitment and inflammation. It is well described that the ECM and immune response are interlinked and mutually participate in driving fibrosis, although their precise interactions in the context of chronic liver disease are poorly understood. This review aims to describe the known pro-/anti-inflammatory and fibrogenic properties of ECM proteins and DAMPs, with particular reference to the immunomodulatory properties of the ECM in the context of chronic liver disease. Finally, we discuss the importance of developing novel biotechnological platforms based on decellularized ECM-scaffolds, which provide opportunities to directly explore liver ECM-immune cell interactions in greater detail.

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Section: Discussionmentioning

confidence: 51%

Section: Ecm In Chronic Liver Diseasementioning

confidence: 97%

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Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

et al. 2020

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“…Cryopreserved in vitro expanded SARS-CoV-2-specific unmodified and FKBP12 KO T cells were thawed and re-stimulated with SARS-CoV-2 peptide pool-loaded LCLs for 6 h and subsequently T cells were purified with the Sony sorter MA900 and cell pellets were frozen in liquid nitrogen and stored at À80 C. Further sample preparation and analysis via nano-liquid chromatography-tandem mass spectrometry was performed as already described elsewhere. 128 The acquired raw files were analyzed by data analysis (Version 3.0, Bruker Daltonic, Bremen, Germany). The derived peak lists were searched against the human Swiss-Prot database using PEAKS studio proteomics software version 7.5 (Bioinformatics Solutions, Waterloo, Canada).…”

Section: Proteomicsmentioning

confidence: 99%

Tacrolimus-resistant SARS-CoV-2-specific T cell products to prevent and treat severe COVID-19 in immunosuppressed patients

Peter

Wendering

Schlickeiser

et al. 2022

Molecular Therapy - Methods & Clinical Development

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“…Human tissue‐specific core matrisome in the context of homeostasis and neoplasia. Tissue‐specific data curated from different studies, including: lung, [ 15 ] lung adenocarcinoma, [ 16 ] brain, [ 17 ] glioblastoma multiforme, [ 16 ] pancreas, [ 18 ] pancreatic ductal adenocarcinoma, [ 19 ] liver, [ 20 ] hepatocellular carcinoma, [ 16 ] kidney, [ 21 ] kidney clear cell carcinoma, [ 16 ] colon, [ 22 ] colorectal cancer, [ 22 ] skin, [ 23 ] and melanoma. [ 22 ] Data obtained from different core matrisome characterization methodologies including proteomics and genomics analysis.…”

Section: Introductionmentioning

confidence: 99%

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Moura

Monteiro

Ferreira

et al. 2022

Adv Funct Materials

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The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next‐generation tissue‐specific hydrogels with close‐to‐native biomolecular signatures and user‐tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue‐specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom‐up cell engineering technologies may unravel unprecedented tissue‐like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient‐tailored living therapeutics.

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The human liver matrisome – Proteomic analysis of native and fibrotic human liver extracellular matrices for organ engineering approaches

Cited by 21 publications

References 44 publications

Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

Tacrolimus-resistant SARS-CoV-2-specific T cell products to prevent and treat severe COVID-19 in immunosuppressed patients

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

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