The Human Immunomodulatory CD25⁺ B Cell Population belongs to the Memory B Cell Pool

Abstract

We have shown that human CD20+25+ B cells display immunomodulatory properties. The aim of this study was to investigate if CD25+ B cells are found within the CD27 memory B cell population, and to analyse pattern of their cytokine production. B cells isolated from healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients were analysed regarding the frequency of CD25+ B cells within certain B cell subsets. Purified CD25+ B cells from healthy subject were used in vitro to evaluate their production of immunomodulatory cytokines. In healthy subjects the majority (60%) of memory B cells (CD20+27+) also co‐expressed CD25 while only 10–20% of the naïve B cells (CD20+27−) and plasmablasts (CD20–27+) expressed CD25. In RA and SLE patients, we found that 51% and 48%, respectively, co‐expressed CD25 in the memory population, whereas only 11% and 9% co‐expressed CD25 in the naïve B cell population. Phenotypic analysis of the CD20+25+27+ and CD20+25+27− cells using CD10, CD24, CD38, CD45, CD71, CD80, CD86, CD95, CD138, BAFF‐R, TACI, IgA, IgD, IgG and IgM showed that CD20+25+27+ B cells preferentially represent highly activated, Ig class switched memory B cells. Cytokine profile analysis showed that CD25+ B cells secreted significantly higher levels of IL‐10 versus CD25− B cells. In contrast, TGF‐β1 secretion was similar between the CD25+ and CD25− sub‐populations. In conclusion, CD20+25+ B cells constitute a unique subpopulation preferentially occurring among CD20+27+ memory B cells. We suggest that CD25 can be used as a marker for a memory B cell subset.

Induction of CD4+ T-cell anergy and apoptosis by activated human B cells

“…25 Based on our data, including selective blocking of IL-2R on the B cell, we suggest that high concentrations of IL-2 might sensitize the lg B25 ϩ cell to its suppressive function and cause a switch from costimulatory to inhibitory signals. Since the CD25 ϩ B cells directly isolated from PB-a majority of them CD27 ϩ memory B cells (see also Amu et al 33 and T.T., R.K.C.V., V.E., R.S., S.S., A.D.H., unpublished observations, 2008)-did not suppress, whereas the less mature, predominantly CD27-naive B cells from PB did, we suggest that maturation status of the B cell represents another critical parameter in this process. This hypothesis is also supported by an observation during our experiments with B-cell stimulatory CpG-rich oligonucleotides (CpG-ODNs): Although after treatment again only the large B-cell fraction (representing up to 40% of the total B-cell population) exhibited a strong inhibitory effect toward CD4 ϩ T cells, comparable with SAC, the great majority of the B cells (Ͼ 80%) expressed CD25 on the cell surface (T.T., R.C.K.V., V.E., R.S., S.S., A.D.H., and H.-M.L., unpublished observations, 2008).…”

B Cell Lymphogenesis in Swine Is Located in the Bone Marrow

“…The differences in expression of CD2 and CD25 are explained by an observation that human B cells generally do not express CD2, and CD25 is expressed only on a fraction of memory B cells (31). In contrast, all mouse B cells are CD2 + (32), and CD25 is expressed early in B cell development (3).…”

B cell immunosenescence: different features of naive and memory B cells in elderly

“…CD27 ? ) (Amu et al 2007) and immature transitional (CD38 high CD24 high ) B cell pools (Blair et al 2010). On the other hand, Bouaziz et al (2010), have shown that both IgD ?…”