The Human Immunoglobulin Genes

“…The position of the PCR primers is indicated. [38][39][40] germline genes were subsequently aligned with the rearranged IgH genes to determine DNA was isolated from 13 intermediate-/high-grade maligthe boundaries of the CDR3 domain, using DNAMAN nant lymphomas that contained one or two rearranged IgH software (Lynnon Biosoft, Quebec, Canada).…”

Section: Figurementioning

confidence: 99%

Detection of minimal disease using rearranged immunoglobulin heavy chain genes from intermediate- and high-grade malignant B cell non-Hodgkin’s lymphoma

et al. 1997

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“…In the course of their maintenance in culture, spontaneous rearrangements of human chromosome 2 occurred in these hybrids, and therefore they contain different portions of this chromosome. These hybrid lines were again characterized by analysis of human chromosome 2 markers malate dehydrogenase 1, which has been mapped to band 2p23 (19), and isocitrate dehydrogenase 1, mapped to 2q32-2qter (19), and by testing of their DNA for hybridization with a fragment of the immunoglobulin K gene, kindly provided by Philip Leder, which has been mapped to human 2p12 (20). DNA from the two humanmouse hybrids A9 Call 1-9-3 and A9 Call 1-13, which contain the chromosome 2 regions qter --p23 and p23 -* pter, respectively, and which were described earlier (8,21), was a generous gift from K.-H. Grzeschik (Munster, Federal Republic of Germany).…”

Section: Methodsmentioning

confidence: 99%

Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines.

Shiloh¹,

Shipley²,

Brodeur³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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DNA amplification, manifested by homogeneously staining regions in chromosomes and by extrachromosomal, double minute bodies, is characteristic of many neuroblastoma cell lines. Sequences recruited from a specific domain on the short arm of chromosome 2 (2p) are amplified in advanced-stage primary neuroblastomas, whereas sequences from distinctly different regions of 2p are amplified in the neuroblastoma cell line IMR-32. Five different DNA segments, which include the oncogene N-myc, three other fragments derived from the homogeneously staining region of the neuroblastoma cell line IMR-32, and a fifth fragment, derived from the neuroblastoma cell line NB-9, showed differential and variable amplification in 24 advanced-stage neuroblastoma tumors out of 112 tested specimens. AU five fragments were mapped within the chromosomal region 2p23-2p25 by three different approaches. However, eight other fragments cloned from the homogeneously staining region of IMR-32 cells, which were not amplified in the tumor tissues examined, were mapped to two more proximal domains of 2p, thousands of kilobases apart from each other and from the chromosomal domain that is amplified in the tumors. These results establish the amplification, to different degrees, of a variable-sized segment of one domain near the terminus of 2p in advanced neuroblastomas. These tumors might ultimately be distinguished according to the pattern of amplification of DNA segments within this domain. The data presented also indicate the existence of a new and complex amplification mechanism in at least one neuroblastoma cell line (IMR-32), which involves not only relocation of DNA from specific genomic domains but also the formation of novel units by splicing together very distant DNA segments.

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The Human Immunoglobulin Genes

Cited by 28 publications

References 0 publications

Report of the committee on the genetic constitution of chromosomes 18, 19, 20, 21, and 22

Report of the committee on the genetic constitution of chromosomes 18, 19, 20, 21, and 22

Detection of minimal disease using rearranged immunoglobulin heavy chain genes from intermediate- and high-grade malignant B cell non-Hodgkin’s lymphoma

Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines.

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