“…Human embryonic stem cells (hESCs) lines: H9 (WA-09, passages 51-80), ES3 (CMR[B], passages [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54], and ES5 (CMR [B], passages 27-55) and a human iPS cell line derived from a healthy individual in our laboratory, iPSC C1 (BP-143) (supplemental online Fig. 1) (passages 7-36), were maintained on irradiated human foreskin fibroblasts (HFF1, SCRC-1041; American Type Culture Collection [ATCC], Manassas, VA, http://www.atcc.org) in human embryonic stem (hES) medium composed of knockout Dulbecco's modified Eagle's medium (DMEM) (10829-018; Invitrogen, Carlsbad, CA, http://www.invitrogen.com), supplemented with 2 mM L-GlutaMAX (35050038; Invitrogen), 50 mM b-mercaptoethanol (31350-010; Invitrogen), 13 nonessential amino acids (M7145; Sigma-Aldrich, St. Louis, MO, http://www.sigmaaldrich.com), 20% knockout serum (KSR; Invitrogen), 50 U/ml penicillin, 50 mg/ml streptomycin, and 10 ng/ml of fibroblast growth factor (FGF) 2 (100-18B; PeproTech).…”