The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors

Murphy, Elizabeth V.; Zhang, Yong; Zhu, Wejia; Biggs, Joseph R.

doi:10.1016/0378-1119(95)00061-a

Cited by 118 publications

(85 citation statements)

References 11 publications

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“…All members of this family share a common CAP domain (also known as a sperm-coating protein, SCP, domain or a PR-1 domain), which is characterized by the presence of two signature motifs that are involved in the formation of a cleft-like structure forming a putative active site containing (in the CRISPs) three intra-molecular disulphide bonds (Henriksen et al, 2001;Shikamoto et al, 2005). CAPs are typically secreted and found in an extraordinary range of species in both bacteria and eukaryotes including, for example, yeast, fungi, plants, cone snails, drosophila, lampreys, snakes, mice, and humans (Pfitzner and Goodman, 1987;Schuren et al, 1993;Miosga et al, 1995;Murphy et al, 1995;Schreiber et al, 1997;Yamazaki et al, 2002b;Milne et al, 2003;Ito et al, 2007)). The presence of such an evolutionarily diverse, yet conserved, structure is suggestive of a common function, although the identification of this function remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Reddy¹,

Gibbs²,

Merriner³

et al. 2008

Developmental Dynamics

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The Cysteine-RIch Secretory Proteins (CRISPs) are abundantly produced in the male reproductive tract of mammals and within the venom of reptiles and have been shown to regulate ion channel activity. CRISPs, along with the Antigen-5 proteins and the Pathogenesis related-1 (Pr-1) proteins, form the CAP superfamily of proteins. Analyses of EST expression databases are increasingly suggesting that mammalian CRISPs are expressed more widely than in the reproductive tract. We, therefore, conducted a reverse transcription PCR expression profile and immunohistochemical analyses of 16 mouse tissues to define the sites of production of each of the four murine CRISPs. These data showed that each of the CRISPs have distinct and sometimes overlapping expression profiles, typically associated with the male and female reproductive tract, the secretory epithelia of exocrine glands, and immune tissues including the spleen and thymus. These investigations raise the potential for a role for CRISPs in general mammalian physiology. Developmental Dynamics 237:3313-3323, 2008.

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Section: Introductionmentioning

confidence: 99%

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Reddy¹,

Gibbs²,

Merriner³

et al. 2008

Developmental Dynamics

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“…13 The human RTVP-1 cDNA was originally cloned from human glioma tissue, 14,15 and subsequently reported to be expressed in differentiated macrophages. 16 In our initial report, we showed that RTVP-1 is regulated by p53 through specific p53-binding sites, and that overexpression of RTVP-1 in vitro leads to apoptosis in multiple mouse and human cancer cell lines and in endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Adenoviral vector-mediated RTVP-1 gene-modified tumor cell-based vaccine suppresses the development of experimental prostate cancer

et al. 2006

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We previously identified a novel p53 target gene, RTVP-1, that possesses unique cytotoxic and immunostimulatory activities which make it potentially useful for cancer gene therapy. To test the therapeutic potential of RTVP-1 in a gene-modified tumor cell-based vaccine model, we used an adenoviral vector capable of efficient transduction and expression of RTVP-1 (AdRTVP-1), together with a highly metastatic mouse prostate cancer cell line (178-2 BMA). A vaccine was prepared with 178-2 BMA cells transduced with AdRTVP-1 or a control adenoviral vector expressing b-galactosidase (Adbgal). After irradiation of the cells, syngeneic 129/Sv mice were vaccinated three times at weekly intervals. After 3 weeks, they were challenged with orthotopic 178-2 BMA cells. After 21 days, fewer than 60% of the RTVP-1-cell-vaccinated mice developed tumors compared to 100% of the control mice. The RTVP-1-cell vaccine significantly reduced primary tumor wet weight compared with control Adbgal-cell vaccine (Po0.0001 at 7 and 14 days). Experimental metastasis to lung was also significantly reduced (P ¼ 0.0377), and survival significantly increased (P ¼ 0.0002). In addition, significantly increased NK and CTL activities were demonstrated in the AdRTVP-1-cell-vaccinated mice. These findings indicate that RTVP-1 gene-modified cell-based vaccines may be useful in the prevention of recurrent prostate cancer.

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“…GliPR was found in all glioma cell lines and tumors studied, but was not detectable in any normal fetal or adult tissues, including normal brain, suggesting that GliPR plays an important role for tumor growth. High levels of GliPR expression can also be induced with phorbol ester in macrophages (1), which are active at the front line of the human immune system. RTVP-1, another protein that was recently found in glioblastoma multiforme (3), is almost identical to GliPR.…”

mentioning

confidence: 99%

Structure comparison of human glioma pathogenesis-related protein GliPR and the plant pathogenesis-related protein P14a indicates a functional link between the human immune system and a plant defense system

Szyperski¹,

Fernández²,

Mumenthaler³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

105

106

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The human glioma pathogenesis-related protein (GliPR) is highly expressed in the brain tumor glioblastoma multiforme and exhibits 35% amino acid sequence identity with the tomato pathogenesis-related (PR) protein P14a, which has an important role for the plant defense system. A molecular model of GliPR was computed with the distance geometry program DIANA on the basis of a P14a-GliPR sequence alignment and a set of 1,200 experimental NMR conformational constraints collected with P14a. The GliPR structure is represented by a group of 20 conformers with small residual DIANA target function values, low AMBERenergies after restrained energy-minimization with the program OPAL, and an average rms deviation relative to the mean of 1.6 Å for the backbone heavy atoms. Comparison of the GliPR model with the P14a structure lead to the identification of a common partially solvent-exposed spatial cluster of four amino acid residues, His-69, Glu-88, Glu-110, and His-127 in the GliPR numeration. This cluster is conserved in all known plant PR proteins of class 1, indicating a common putative active site for GliPR and PR-1 proteins and thus a functional link between the human immune system and a plant defense system.

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The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors

Cited by 118 publications

References 11 publications

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Adenoviral vector-mediated RTVP-1 gene-modified tumor cell-based vaccine suppresses the development of experimental prostate cancer

Structure comparison of human glioma pathogenesis-related protein GliPR and the plant pathogenesis-related protein P14a indicates a functional link between the human immune system and a plant defense system

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