The human endosalpinx: anatomical three-dimensional study and reconstruction using confocal microtomography

Abstract

Purpose To evaluate in three dimensions (3D) the human endosalpinx and reconstruct its surface along its different anatomical segments, without the injection or insertion of luminal contrasts, using confocal microtomography (micro-CT). Material and methods 15 fallopian tubes (FT) from 14 women in reproductive age from procedures for benign disease or sterilization were selected. The specimens were fixed in formalin and stained with Lugol solution. Micro-CT studies were conducted on the specimens using protocols adapted from biological studies, to acquire images to reconstruct in 3D the endosalpinx surface. Results From these specimens, 6 presented the intra-mural segment, 14 presented the isthmus and 15 presented the ampulla and fimbria segment of the FT. The specimen presented tissue definition, and contrast sufficient for FT endosalpinx morphological analysis and lumen definition. The intramural portion presented initially a mucosal projection toward the lumen, bending on its own axis, and increased numbers of projections towards the isthmic portion, where the projections become longer more numerous. The endosalpinx becomes more tortuous, the lumen diameter increases and the mucosal projections become more bulky in the ampullary portion, with the projections less present on the antimesenteric side. The infundibular portion is marked with the organized and predictable endosalpinx, the abdominal ostium is cleared demonstrated, with the reduction of the endosalpinx volume. The fimbria demonstrated a small relation between fringes and intratubal endosalpinx. Conclusions Microscopic anatomy of different segments of the human FT mucosa can be analyzed and reconstructed in 3D with histological correlation using micro-CT.

Fallopian tube: Three‐dimensional reconstruction and virtual navigation using microtomography

“…Osvaldo Luiz Aranda MD 2 Gerson Ribeiro MD 3 Edward Araujo Júnior PhD 4 Alessandra Silveira Machado PhD 5 Heron Werner PhD 1 1 Fetal Medicine Department, Clínica Diagn ostico por Imagem (CDPI),…”

“…After approval from the appropriate ethical committee, one normal FT and one segment of FT after tubal ligation (FTATL) were immersed in Lugol contrast, as previously described. 3 Images were captured using the Phoenix VTomeX (General Electric, Wunstorf, Germany) and the SkyScan 1173 (v.1.6.9.4; Bruker microCT, Kontich, Belgium) with the parameters of energy and time of X-ray exposure, as the number of images captured adapted for each specimen. The The FT demonstrates diminished thickness of the muscular segment of the FTATL.…”