The human DNA content in artifacts deposited by the blowfly Lucilia cuprina fed human blood, semen and saliva

Durdle, Annalisa; Mitchell, Robert J.; Oorschot, Roland A.H. van

doi:10.1016/j.forsciint.2013.09.015

Cited by 24 publications

(42 citation statements)

References 26 publications

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“…Among these, 7 were case reports [13][14][15][16][17][18][19], 3 case series [6,20,21], 19 original articles [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40], 4 were technical notes [41][42][43][44] and 8 were reviews [1,10,[45][46][47][48][49][50].…”

Section: Resultsmentioning

confidence: 99%

“…blood, semen, saliva) [30]. DNA can be extracted from FAs derived from blood and semen for a period of at least 2 years, and from saliva for 2 months after deposition [27,28]. As a result, artefacts can be a valuable source of DNA for investigators in cases where the victim's body was removed and/or the offender attempted to clean up any biological evidence due to the ability to be sampled a long time after deposition and far from the crime scene.…”

Section: Fly Artefactsmentioning

confidence: 99%

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Crime scene and body alterations caused by arthropods: implications in death investigation

et al. 2018

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The activity of arthropods on corpses has been largely investigated, since they can produce information to reconstruct the perimortem events. However, the feeding/movement activity of insects around the crime scene, among the clothes and on the body, can also cause some alterations that can lead to wrong reconstruction and misinterpretations. This article summarises all the postmortem arthropods artefacts related to the scene (i.e. fly artefacts and floor stripes) and the body (i.e. skin and other soft tissue alterations, bone alterations and hair alterations) that can mislead the forensic pathologist, discussing macroscopic and microscopic findings derived from forensic casework and from experimental laboratory studies, in order to provide a useful instrument to avoid misinterpretations and evaluation errors. Finally, some procedural notes for the documentation and the interpretation of findings are proposed.

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Section: Resultsmentioning

confidence: 99%

Section: Fly Artefactsmentioning

confidence: 99%

Crime scene and body alterations caused by arthropods: implications in death investigation

et al. 2018

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“…It is the latter that is most frequently cited by forensic entomologists, since both regurgitation and fecal elimination can yield insect stains containing human blood. The reality is that necrophagous flies can produce stains or artifacts as a result of feeding on several types of fluids (e.g., blood, saliva, semen, vaginal fluids, decomposition fluids), and which yield artifacts that vary widely in terms of shape, color, and size [12,14,19,20]. Deposition of artifacts is also not restricted to just foraging adults, as post-feeding larvae and newly emerged adults have the potential to contaminate crime scenes with unique artifacts.…”

Section: Insect Artifactsmentioning

confidence: 99%

“…Stains resulting from fecal release are the most commonly produced artifacts by L. cuprina and C. vicina (collected in Frankfurt, Germany) following feeding on human blood [6,14]. In contrast, regurgitate stains are the most abundant artifacts released by S. bullata , L. sericata , Ch.…”

Section: Insect Artifactsmentioning

confidence: 99%

“…Fly stains are morphologically very similar to impact (i.e., forward, back, and mist-like spatter), projected, sneezed, and expirated bloodstains [10], and cannot be reliably distinguished using presumptive or confirmatory tests available for identification of human blood [13,14,15]. The use of molecular methods, namely DNA typing, for person’s identification does not overcome these limitations since complete DNA profiles can be obtained of an individual from blood consumed by flies [6,16].…”

Section: Introductionmentioning

confidence: 99%

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Insect Artifacts Are More than Just Altered Bloodstains

Rivers

Geiman

2017

Insects

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The bases for forensic entomology are that insects and their arthropod relatives can serve as evidence in criminal, medical and civil legal matters. However, some of the very same species that provide utility to legal investigations can also complicate crime scenes by distorting existing body fluid evidence (e.g., bloodstains, semen, saliva) and/or depositing artifacts derived from the insect alimentary canal at primary or secondary crime scenes. The insect contaminants are referred to as insect stains, artifacts, specks or spots, and are most commonly associated with human bloodstains. This review will discuss the different types of insect artifacts that have been described from crime scenes and laboratory experiments, as well as examine insect contaminates (non-blood based artifacts, transfer patterns, meconium, and larval fluids) that have received little research or case attention. Methods currently used for distinguishing insect stains from human body fluids will also be discussed and compared to presumptive tests used for identification of human body fluids. Since all available methods have severe limitations, areas of new research will be identified for the purpose of development of diagnostic techniques for detection of insect artifacts.

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Emerging use of air eDNA and its application to forensic investigations – A review

Goray,

Taylor,

Bibbo

et al. 2024

Electrophoresis

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Biological material is routinely collected at crime scenes and from exhibits and is a key type of evidence during criminal investigations. Improvements in DNA technologies allow collection and profiling of trace samples, comprised of few cells, significantly expanding the types of exhibits targeted for DNA analysis to include touched surfaces. However, success rates from trace and touch DNA samples tend to be poorer compared to other biological materials such as blood. Simultaneously, there have been recent advances in the utility of environmental DNA collection (eDNA) in identification and tracking of different biological organisms and species from bacteria to naked mole rats in different environments, including, soil, ice, snow, air and aquatic. This paper examines the emerging methods and research into eDNA collection, with a special emphasis on the potential forensic applications of human DNA collection from air including challenges and further studies required to progress implementation.

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The human DNA content in artifacts deposited by the blowfly Lucilia cuprina fed human blood, semen and saliva

Cited by 24 publications

References 26 publications

Crime scene and body alterations caused by arthropods: implications in death investigation

Crime scene and body alterations caused by arthropods: implications in death investigation

Insect Artifacts Are More than Just Altered Bloodstains

Emerging use of air eDNA and its application to forensic investigations – A review

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