The human coronary vasodilatory response to acute mental stress is mediated by neuronal nitric oxide synthase

Khan, Safia; Melikian, Narbeh; Shabeeh, Husain; Cabaco, Ana Rita; Martin, Katherine J.; Khan, Faisal; O’Gallagher, Kevin; Chowienczyk, Phil; Shah, Ajay M.

doi:10.1152/ajpheart.00745.2016

Cited by 13 publications

(9 citation statements)

References 44 publications

(58 reference statements)

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“…PRG1-1 treatment increased the expression levels of iNOS and COX-2, both of which are associated with NO production. The release of NO is involved in many physiological processes, such as vasodilatation (Khan et al, 2017), neurotransmission (Kusek et al, 2017), immune responses (Cinzia, Maria Pia, Angela, & Marco, 2016), and platelet aggregation (Smyth et al, 2017). Furthermore, activated macrophages produce cytokines, including IL-6 and TNF-α, that play important roles in the cellular immune process by aiding in the elimination of abnormal cells (Ying et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Immune-enhancing effects of a polysaccharide PRG1-1 from Russula griseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways

Chen

Gong

et al. 2018

Food and Agricultural Immunology

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Immune-enhancing effects of a polysaccharide PRG1-1 from Russulagriseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways, Food and Agricultural Immunology, 29:1, 833-844, ABSTRACTPolysaccharides are one of many bioactive compounds found in edible mushrooms. Edible mushrooms have become attractive as "health foods" and as source materials for immunomodulators. The aim of this project was to study the immunoregulatory effects of a purified polysaccharide derived from wild Russula griseocarnosa (PRG1-1) on macrophages. Our data showed that in RAW264.7 macrophage cells, PRG1-1 increased expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, PRG1-1 increased the production of nitric oxide (NO) and cytokines, including interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α). Western blotting demonstrated that the regulation of NO and cytokines was mediated through the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signalling pathways. Therefore, PRG1-1 has the capacity to activate macrophages via the NF-κB and MAPK pathways. These findings helped to elucidate the immunemodulatory properties of the polysaccharide from R. griseocarnosa. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Immune-enhancing effects of a polysaccharide PRG1-1 from Russula griseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways

Chen

Gong

et al. 2018

Food and Agricultural Immunology

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“…Indeed, an nNOS-selective inhibitor, S-methyl-L-thiocitrulline (SMTC), reduced basal CBF, and epicardial coronary diameter ( Seddon et al., 2009 ). Recently, it has been established that nNOS plays a role in mental stress-induced vasodilatation in human coronary circulation ( Khan et al., 2017 ). In contrast, SMTC had no effect on substance P- and pacing-induced increases in CBF, although these responses were significantly attenuated by the nonselective NOS inhibitor L-NMMA, suggesting the involvement of eNOS but not nNOS ( Seddon et al., 2009 ; Shabeeh et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

L-Arginine-Nitric Oxide-Asymmetric Dimethylarginine Pathway and the Coronary Circulation: Translation of Basic Science Results to Clinical Practice

et al. 2020

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FIGURE 1 | This figure shows a simplified overview of nitric oxide (NO) pathway with most of the molecular steps. The substrate for nitric oxide synthases, the amino-acid L-arginine can enter into three main metabolic pathways. It is either used by arginase, producing urea, and L-ornithine or used by nitric oxide synthase (NOS) to produce NO or methylated, producing methylated L-arginines, which being a false substrate inhibit NOS. Other metabolic pathways are connected that are modifying the level of NO or affected by NO. Czirá ki et al. Nitric Oxide and Coronary Circulation

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“…It has been shown that the Stroop task excites cardiopulmonary baroreceptors which then attenuate sympathetic outflow to the forearm vasculature causing muscle vasodilation [29]. Other Stroop-induced vasodilatory mechanisms such as Stroop-induced elevations in shear stress causing release of vasodilatory substances such as nitric oxide [28,30] may also have contributed to the rise in skeletal muscle blood flow and vascular conductance.…”

Section: Discussionmentioning

confidence: 99%

Acute Effect of Aerobic Exercise on Cardiovascular Reactivity of Overweight Males

Chandrakumar¹,

Dyck²,

Boutcher³

et al. 2018

saj

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Overweight compared to normal weight individuals typically possess reduced limb blood flow at rest and during mental challenge. The effect of a single bout of aerobic exercise on cardiovascular reactivity of young overweight males, however, is undetermined. Thus, the purpose was to examine the cardiovascular reactivity response of overweight males to mental challenge before and after acute aerobic exercise. Fifteen normal weight (NW) males with body mass index (BMI) between 18 and 25 kg/m 2 and 15 overweight males (OW) with BMI > 25 kg/m 2 aged 18-30 years acted as participants. All males underwent a 5-min Stroop task before and after one single 30-min bout of cycle exercise at 65% of maximal oxygen uptake (V O 2max). Heart rate, blood pressure, arterial stiffness, and forearm blood flow (FBF) were assessed during Stroop task before and after acute exercise. Arterial stiffness of the OW was significantly higher than NW during Stroop before (p =0.01) and after exercise (p = 0.001). After exercise, the OW had significantly smaller increase in FBF throughout Stroop (p = 0.04) compared to NW. The smaller increase in FBF throughout the Stroop in the OW was also accompanied by higher Stroop errors compared to that of NW (p = 0.01). Stroop errors were positively correlated with BMI, waist circumference, waist/hip ratio, and body fat percentage, whereas post-exercise FBF was positively correlated with V O 2max (p < 0.05). Being overweight was associated with a blunted FBF reactivity response and a greater number of errors during the Stroop task.

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The human coronary vasodilatory response to acute mental stress is mediated by neuronal nitric oxide synthase

Abstract: Acute mental stress induces vasodilation of the coronary microvasculature. Here, we show that this response involves neuronal nitric oxide synthase in the human coronary circulation.

Cited by 13 publications

References 44 publications

Immune-enhancing effects of a polysaccharide PRG1-1 from Russula griseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways

Immune-enhancing effects of a polysaccharide PRG1-1 from Russula griseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways

L-Arginine-Nitric Oxide-Asymmetric Dimethylarginine Pathway and the Coronary Circulation: Translation of Basic Science Results to Clinical Practice

Acute Effect of Aerobic Exercise on Cardiovascular Reactivity of Overweight Males

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