The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29

Young, James D.; Yao, Sylvia Y. M.; Baldwin, Jocelyn M.; Cass, Carol E.; Baldwin, Stephen A.

doi:10.1016/j.mam.2012.05.007

Cited by 299 publications

(388 citation statements)

References 88 publications

Supporting

Mentioning

369

Contrasting

Unclassified

Order By: Relevance

“…ENT1 and ENT2 have a fairly ubiquitous cell surface expression and are capable of mediating adenine (and hypoxanthine) transport across the blood brain barrier [126][127][128][129]. It is also likely that allopurinol enters the brain in this way, given its closely related structure to hypoxanthine (Fig.…”

Section: Penetration Into the Brainmentioning

confidence: 99%

The Purine Salvage Pathway and the Restoration of Cerebral ATP: Implications for Brain Slice Physiology and Brain Injury

Frenguelli

2017

Neurochem Res

View full text Add to dashboard Cite

Brain slices have been the workhorse for many neuroscience labs since the pioneering work of Henry McIlwain in the 1950s. Their utility is undisputed and their acceptance as appropriate models for the central nervous system is widespread, if not universal. However, the skeleton in the closet is that ATP levels in brain slices are lower than those found in vivo, which may have important implications for cellular physiology and plasticity. Far from this being a disadvantage, the ATP-impoverished slice can serve as a useful and experimentally-tractable surrogate for the injured brain, which experiences similar depletion of cellular ATP. We have shown that the restoration of cellular ATP in brain slices to in vivo values is possible with a simple combination of d-ribose and adenine (RibAde), two substrates for ATP synthesis. Restoration of ATP in slices to physiological levels has implications for synaptic transmission and plasticity, whilst in the injured brain in vivo RibAde shows encouraging positive results. Given that ribose, adenine, and a third compound, allopurinol, are all separately in use in man, their combined application after acute brain injury, in accelerating ATP synthesis and increasing the reservoir of the neuroprotective metabolite, adenosine, may help reduce the morbidity associated with stroke and traumatic brain injury.

show abstract

Section: Penetration Into the Brainmentioning

confidence: 99%

The Purine Salvage Pathway and the Restoration of Cerebral ATP: Implications for Brain Slice Physiology and Brain Injury

Frenguelli

2017

Neurochem Res

View full text Add to dashboard Cite

show abstract

“…We identified three nucleoside transporters, namely hENT1, hENT2, and hCNT3, as AICAr transporters. The nucleoside transporters constitute a multiprotein family with two subgroups (32): SLC28A (three members, hCNT1-3) and SLC29A (four members, hENT1-4), with each transporter presenting various substrate specificity and sensitivity to inhibitors (32). The remaining members of the family were not able to stimulate AICAr uptake in yeast (hCNT1 and hCNT2, Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of Yeast and Human 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAr) Transporters

Ceschin

Saint-Marc

Laporte

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Additionally, there is a close relation between genomic organization, substrate specificity, and phylogenetic distribution within the Pnu transporter family. Pnu transporters have no homologs in eukaryotes, which use very different transport systems to import the same substrates (Yonezawa and Inui, 2013;Young et al, 2013;Zhao and Goldman, 2013). Pnu transporters are also not related to the prokaryotic substrate binding components of ECFtype ABC transporters, neither in their mechanism of action nor in sequence and three-dimensional structure (Slotboom, 2014).…”

Section: Tracking Pnu Transporter Homologsmentioning

confidence: 99%

Structure, function, evolution and application of bacterial Pnu-type vitamin transporters

Jaehme

Slotboom

2015

Biological Chemistry

View full text Add to dashboard Cite

Many bacteria can take up vitamins from the environment via specific transport machineries. Uptake is essential for organisms that lack complete vitamin biosynthesis pathways, but even in the presence of biosynthesis routes uptake is likely preferred, because it is energetically less costly. Pnu transporters represent a class of membrane transporters for a diverse set of B-type vitamins. They were identified 30 years ago and catalyze transport by the mechanism of facilitated diffusion, without direct coupling to ATP hydrolysis or transport of coupling ions. Instead, directionality is achieved by metabolic trapping, in which the vitamin substrate is converted into a derivative that cannot be transported, for instance by phosphorylation. The recent crystal structure of the nicotinamide riboside transporter PnuC has provided the first insights in substrate recognition and selectivity. Here, we will summarize the current knowledge about the function, structure, and evolution of Pnu transporters. Additionally, we will highlight their role for potential biotechnological and pharmaceutical applications.

show abstract

The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29

Cited by 299 publications

References 88 publications

The Purine Salvage Pathway and the Restoration of Cerebral ATP: Implications for Brain Slice Physiology and Brain Injury

The Purine Salvage Pathway and the Restoration of Cerebral ATP: Implications for Brain Slice Physiology and Brain Injury

Identification of Yeast and Human 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAr) Transporters

Structure, function, evolution and application of bacterial Pnu-type vitamin transporters

Contact Info

Product

Resources

About