The Human Apolipoprotein L Gene Cluster: Identification, Classification, and Sites of Distribution

Page, Nigel M.; Butlin, David; Lomthaisong, Khomsorn; Lowry, P. J.

doi:10.1006/geno.2001.6534

Cited by 122 publications

(118 citation statements)

References 20 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…Within the circulation of primates, apoL-1 is exclusively associated with HDL, and the majority of the protein is in the TLF subclass (5). The apoL family members are all predicted to adopt amphipathic ␣-helical conformations, suggesting that their physiological role involves membrane interaction (20). Apolipoprotein L-1 shares limited homology with channel-forming colicins and, consistent with this observation, has been shown to function as an ion channel when incorporated into lipid bilayers (23).…”

mentioning

confidence: 77%

Membrane Permeabilization by Trypanosome Lytic Factor, a Cytolytic Human High Density Lipoprotein

Harrington

Howell

Hajduk

2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

Trypanosome lytic factor (TLF) is a subclass of human high density lipoprotein (HDL) that mediates an innate immune killing of certain mammalian trypanosomes, most notably Trypanosoma brucei brucei, the causative agent of a wasting disease in cattle. Mechanistically, killing is initiated in the lysosome of the target trypanosome where the acidic pH facilitates a membranedisrupting activity by TLF. Here we utilize a model liposome system to characterize the membrane binding and permeabilizing activity of TLF and its protein constituents, haptoglobinrelated protein (Hpr), apolipoprotein L-1 (apoL-1), and apolipoprotein A-1 (apoA-1). We show that TLF efficiently binds and permeabilizes unilamellar liposomes at lysosomal pH, whereas non-lytic human HDL exhibits inefficient permeabilizing activity. Purified, delipidated Hpr and apoL-1 both efficiently permeabilize lipid bilayers at low pH. Trypanosome lytic factor, apoL-1, and apoA-1 exhibit specificity for anionic membranes, whereas Hpr permeabilizes both anionic and zwitterionic membranes. Analysis of the relative particle sizes of susceptible liposomes reveals distinctly different membrane-active behavior for native TLF and the delipidated protein components. We propose that lysosomal membrane damage in TLF-susceptible trypanosomes is initiated by the stable association of the TLF particle with the lysosomal membrane and that this is a property unique to this subclass of human HDL.

show abstract

mentioning

confidence: 77%

Membrane Permeabilization by Trypanosome Lytic Factor, a Cytolytic Human High Density Lipoprotein

Harrington

Howell

Hajduk

2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…GAT-3Ј) genes, as described (20). Human total RNA was obtained from a human total RNA master panel (BD Biosciences Clontech) or extracted directly from tissues and cells by using Tri-reagent and converted to SMART 3Ј-RACE-ready cDNA as described (20).…”

mentioning

confidence: 99%

“…Human total RNA was obtained from a human total RNA master panel (BD Biosciences Clontech) or extracted directly from tissues and cells by using Tri-reagent and converted to SMART 3Ј-RACE-ready cDNA as described (20). The cDNA levels were normalized and confirmed by PCR amplification of the h␤-actin (sense 5Ј-TCCATCATGAAGTGTGACGTGGACATC-3Ј, antisense 5Ј-CCAGACTCGTCATACTCCTGCTTGCTG-3Ј) and hGAPDH genes (sense 5Ј-AGGCTGAGAACGGGAAGCTTGTGATCA-3Ј, antisense 5Ј-TCCCGTTCAGCTCAGGGATGACCTTGC-3Ј) for 18 cycles.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the endokinins: Human tachykinins with cardiovascular activity

Page

Bell

Gardiner

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

143

161

View full text Add to dashboard Cite

We report four human tachykinins, endokinins A, B, C, and D (EKA-D), encoded from a single tachykinin precursor 4 gene that generates four mRNAs (␣, ␤, ␥, and ␦). Tachykinin 4 gene expression was detected primarily in adrenal gland and in the placenta, where, like neurokinin B, significant amounts of EKB-like immunoreactivity were detected. EKA͞B 10-mers displayed equivalent affinity for the three tachykinin receptors as substance P (SP), whereas a 32-mer N-terminal extended form of EKB was significantly more potent than EKA͞B or SP. EKC͞D, which possess a previously uncharacterized tachykinin motif, FQGLL-NH2, displayed low potency. EKA͞B displayed identical hemodynamic effects to SP in rats, causing short-lived falls in mean arterial blood pressure associated with tachycardia, mesenteric vasoconstriction, and marked hindquarter vasodilatation. Thus, EKA͞B could be the endocrine͞paracrine agonists at peripheral SP receptors and there may be as yet an unidentified receptor(s) for EKC͞D.

show abstract

“…Apolipoprotein L1 (APOL1) was first identified and characterised in 1997 (Duchateau et al, 1997) and in 2001, we reported the cloning of five further apolipoprotein L (APOL) proteins (Page et al, 2001) yielding a new gene family consisting of six proteins (APOL1 to 6) (Page et al, 2001;Duchateau et al, 2001). Although the precise functions of APOL proteins have not as yet been fully described, each APOL protein shares significant identity within their predicted amphipathic alpha helices (Page et al, 2001) in areas which is suggestive of a role in lipid binding (Duchateau et al, 1997;Page et al, 2001).…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Polymorphisms in the Apolipoprotein L1 gene and their effects on blood lipid and glucose levels in middle age males

et al. 2006

Self Cite

View full text Add to dashboard Cite

The Human Apolipoprotein L Gene Cluster: Identification, Classification, and Sites of Distribution

Cited by 122 publications

References 20 publications

Membrane Permeabilization by Trypanosome Lytic Factor, a Cytolytic Human High Density Lipoprotein

Membrane Permeabilization by Trypanosome Lytic Factor, a Cytolytic Human High Density Lipoprotein

Characterization of the endokinins: Human tachykinins with cardiovascular activity

Polymorphisms in the Apolipoprotein L1 gene and their effects on blood lipid and glucose levels in middle age males

Contact Info

Product

Resources

About