The human and mouse repertoire of the adhesion family of G-protein-coupled receptors

Bjarnadóttir, Thóra K.; Fredriksson, Robert; Höglund, Pär J.; Gloriam, David E.; Lagerström, Malin C.; Schiöth, Helgi B.

doi:10.1016/j.ygeno.2003.12.004

Cited by 208 publications

(221 citation statements)

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“…[31][32][33]. It is difficult to know exactly why these genes have not previously been identified, but this could probably be related to the usage of different gene finding programs to generate ab initio sets as well as the different thresholds used for identifying EST evidence for the predicted genes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

et al. 2004

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Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human genes can be found. The results indicate that there are about 25 000 unique genes in the union of RefSeq and Ensembl with 12-18% and 8-13% of the genes in each set unique to the other set, respectively. About 20% of all genes had splice variants. There are a considerable number of ESTs (2 200 000) that do not match the identified genes and we used an in-house pipeline to identify 22 novel genes from Genscan predictions that have considerable EST coverage. The study provides an insight into the current status of human gene catalogues and shows that considerable refinement of methods and datasets is needed to come to a conclusive gene count.

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Section: Discussionmentioning

confidence: 99%

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

et al. 2004

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“…(Fredriksson et al, 2003). Further subgrouping of the adhesion class based on their transmembrane regions and N-terminal domain sequences identified nine aGPCR groups (I-IX), which are present in mammalian genomes (Bjarnadottir et al, 2004). aGPCR group VI encompasses five predicted aGPCR, Gpr110, Gpr111, Gpr113, Gpr115, and Gpr116.…”

Section: Developmental Dynamicsmentioning

confidence: 99%

“…From a phylogenetic perspective, they define the second largest class within the GPCR superfamily. Mammalian genomes possess 33 identified members clustered in nine groups (Bjarnadottir et al, 2004). aGPCR can already be distinguished in unicellular eukaryotes and primitive animals rendering them one of the oldest GPCR designs in evolution (Nordstrom et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional study of a cluster of four highly conserved orphan adhesion‐GPCR in mouse

Prömel

Waller-Evans

Dixon

et al. 2012

Developmental Dynamics

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Background: Adhesion G protein-coupled receptors (aGPCR) constitute a structurally and functionally diverse class of seven-transmembrane receptor proteins. Although for some of the members important roles in immunology, neurology, as well as developmental biology have been suggested, most receptors have been poorly characterized. Results: We have studied evolution, expression, and function of an entire receptor group containing four uncharacterized aGPCR: Gpr110, Gpr111, Gpr115, and Gpr116. We show that the genomic loci of these four receptors are clustered tightly together in mouse and human genomes and that this cluster likely derives from a single common ancestor gene. Using transcriptional profiling on wild-type and knockout/LacZ reporter knockin mice strains, we have obtained detailed expression maps that show ubiquitous expression of Gpr116, co-expression of Gpr111 and Gpr115 in developing skin, and expression of Gpr110 in adult kidney. Loss of Gpr110, Gpr111, or Gpr115 function did not result in detectable defects, indicating that genes of this aGPCR group might function redundantly. Conclusions: The aGPCR cluster Gpr110, Gpr111, Gpr115, and Gpr116 developed from one common ancestor in vertebrates. Expression suggests a role in epithelia, and one can speculate about a possible redundant function of GPR111 and GPR115. Developmental Dynamics 241:1591-1602, 2012.V C 2012 Wiley Periodicals, Inc.

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“…1A) [7][8][9]. Together with CD97 and EMR2 through EMR4, it belongs to the EGF-TM7 family, a subfamily within the adhesion class of TM7 receptors [10,11]. All these receptors have variable numbers of EGF-like domains, which can mediate ligand binding [12,13].…”

Section: Introductionmentioning

confidence: 99%

EMR1, the human homolog of F4/80, is an eosinophil‐specific receptor

et al. 2007

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The EGF-TM7 F4/80 is a defining marker of murine macrophage populations. Applying flow cytometric analysis using the newly generated mAb A10, and quantitative real-time PCR, we here report the surprising observation that the human ortholog of F4/80, EGFlike module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells. Unexpectedly, we found that EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans.

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The human and mouse repertoire of the adhesion family of G-protein-coupled receptors

Cited by 208 publications

References 20 publications

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery

Characterization and functional study of a cluster of four highly conserved orphan adhesion‐GPCR in mouse

EMR1, the human homolog of F4/80, is an eosinophil‐specific receptor

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