The human adipose tissue is a source of multipotent stem cells

Rodriguez, Anne‐Marie; Elabd, Christian; Amri, Ez-Zoubir; Ailhaud, Gérard; Dani, Christian

doi:10.1016/j.biochi.2004.11.007

Cited by 355 publications

(237 citation statements)

References 27 publications

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“…However, we did not optimize culture conditions for other cell lineages and thus additional work is required to characterize the physiology of those cells. Our findings of increasing adipokine concentrations and glycerol release over the course of differentiation indicate commitment to the adipogenic lineage and confirm that brown bear adipocyte differentiation is similar to in vitro adipogenic differentiation in other mammals (Rodriguez et al 2005). Adiponectin production is specific to mature adipocytes (Körner et al 2005) while leptin is produced in several tissues with greatest expression in adipocytes (Margetic et al 2002).…”

Section: Discussionsupporting

confidence: 79%

A protocol for the isolation and cultivation of brown bear (Ursus arctos) adipocytes

et al. 2016

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Brown bears (Ursus arctos) exhibit hyperphagia each fall and can become obese in preparation for hibernation. They do this without displaying the physiological problems typically seen in obese humans, such as Type 2 diabetes and heart disease. The study of brown bear hibernation biology could therefore aid in the development of novel methods for combating metabolic diseases. To this end, we isolated mesenchymal stem cells from subcutaneous fat biopsies, and culture methods were developed to differentiate these into the adipogenic lineage. Biopsies were taken from 8 captive male (N = 6) and female (N = 2) brown bears, ages 2-12 years. Plastic adherent, fibroblast-like cells were proliferated and subsequently cryopreserved or differentiated. Differentiation conditions were optimized with respect to fetal bovine serum content and time spent in differentiation medium. Cultures were characterized through immunostaining, RT-qPCR, and Oil red O staining to quantify lipid accumulation. Adiponectin, leptin, and glycerol medium concentrations were also determined over the course of differentiation. The culturing protocol succeeded in generating hormone-sensitive lipase-expressing, lipid-producing white-type adipocytes (UCP1 negative). Serum concentration and time of exposure to differentiation medium were both positively related to lipid production. Cells cultured to low passage numbers retained similar lipid production and expression of lipid markers PLIN2 and FABP4. Ultimately, the protocols described here may be useful to biologists in the field investigating the health of wild bear populations and could potentially increase our understanding of metabolic disorders in humans.

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Section: Discussionsupporting

confidence: 79%

A protocol for the isolation and cultivation of brown bear (Ursus arctos) adipocytes

et al. 2016

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“…Especially, white adipose tissue can be obtained from large reservoir in human, and resident stem cells are easily collected from white adipose tissue with the ability to differentiate into multiple mesenchymal lineages. [3][4][5][6] As Green et al 7 reported that adipose tissue contains 'Preadipocytes' in 1974, a number of investigations have been reported regarding adipose tissue-derived stem/progenitor cells, so-called 'adipose-derived stromal cells (ADSCs). [3][4][5]8 Other adipogenic cell populations 3,9,10 have also been established with the multipotency of differentiation into not only mesenchymal lineage cells such as osteoblasts, 11,12 chondrocytes, 11,13,14 myocytes, 12 cardiomyocytes, 15 fibroblasts 3 and adipocytes 12 but also vascular lineage cells 15,16 such as endothelial, smooth muscle and circulating blood cells in stromal vascular fraction (SVF) isolated from adipose tissue.…”

mentioning

confidence: 99%

Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis

Shoji

Masaaki

Mifune

et al. 2010

Laboratory Investigation

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Adipose tissue is one of the promising sources of multipotent stem cells in human. Human multipotent adipose-derived stem (hMADS) cells have recently been isolated and showed differentiation potential into multiple mesenchymal lineages in vitro and in vivo. On the basis of these evidences, we examined the therapeutic efficacy of hMADS cells for fracture healing in an immunodeficient rat femur non-union fracture model. Local transplantation of hMADS cells radiographically and histologically promoted fracture healing with significant improvement of biomechanical function at the fracture sites compared with local transplantation of human fibroblasts (hFB) or PBS administration. Histological capillary density and physiological blood flow by laser Doppler perfusion imaging were significantly greater in hMADS group than hFB and PBS groups. Expressions of intrinsic (rat) bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and angiopoietin-1 in peri-fracture tissue were upregulated in hMADS group than other groups. In addition, presence of BMP-2 or VEGF activated the proliferation and migration of hMADS cells in vitro. These results indicate that hMADS cells stimulate the interaction between the transplanted cells and the resident cells stronger than other cells, and they promote fracture healing more effectively. Furthermore, immunohistochemistry for human-specific antibodies revealed direct differentiation of hMADS cells into osteoblasts or endothelial cells in newly formed callus or vasculature, respectively. RT-PCR for human-specific primers for osteogenic/endothelial markers also disclosed osteogenic and vasculogenic plasticity of the transplanted hMADS cells at the early stage of fracture healing. The present results suggest that transplantation of hMADS cells may become a useful strategy for cell-based bone regeneration in the future clinical setting.

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“…Adipose tissue is a easily accessible and abundant source of MSCs (Rodriguez et al 2005;Zuk et al 2002). Timper et al (2006) noticed that human adipose derived stem cells (ADSCs) are able to adopt a pancreatic endocrine cell phenotype ex vivo.…”

Section: Differentiation Of Adipose Derived Stem Cellsmentioning

confidence: 99%

Differentiation of Stem Cells into Insulin-Producing Cells: Current Status and Challenges

Pokrywczyńska

Krzyzanowska

Jundziłł

et al. 2013

Arch. Immunol. Ther. Exp.

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Diabetes mellitus is one of the most serious public health challenges of the twenty-first century. Allogenic islet transplantation is an efficient therapy for type 1 diabetes. However, immune rejection, side effects of immunosuppressive treatment as well as lack of sufficient donor organs limits its potential. In recent years, several promising approaches for generation of new pancreatic b cells have been developed. This review provides an overview of current status of pancreatic and extra-pancreatic stem cells differentiation into insulin-producing cells and the possible application of these cells for diabetes treatment. The PubMed database was searched for English language articles published between 2001 and 2012, using the keyword combinations: diabetes mellitus, differentiation, insulin-producing cells, stem cells.

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The human adipose tissue is a source of multipotent stem cells

Cited by 355 publications

References 27 publications

A protocol for the isolation and cultivation of brown bear (Ursus arctos) adipocytes

A protocol for the isolation and cultivation of brown bear (Ursus arctos) adipocytes

Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis

Differentiation of Stem Cells into Insulin-Producing Cells: Current Status and Challenges

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