The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells

Nagaya, Shingo; Kawamura, Kazue; Shinmyo, Atsuhiko; Kato, Ko

doi:10.1093/pcp/pcp188

Cited by 183 publications

(193 citation statements)

References 17 publications

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“…Approximately 250 bp of the 39 region of Arabidopsis HSP18.2 (At5g59720) (Nagaya et al, 2010) was amplified using Xba_HSP_F and Bam_HSP_R primers and inserted between the XbaI and BamHI sites of pGL4.10 vector (Promega). Annealed primers for the five repeats of the G-box and MBS, 418-bp region of JAZ3 promoter, or 1000-bp region of TAT1 promoter were inserted between the SacI and XhoI sites, SalI site, or SacI and XhoI sites of pGL4.1HSP, respectively.…”

Section: Preparation Of Constructsmentioning

confidence: 99%

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

et al. 2013

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Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loophelix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.

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Section: Preparation Of Constructsmentioning

confidence: 99%

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

et al. 2013

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“…A new vector containing the terminator sequence of a heat shock protein (HSP) gene was prepared as follows. A DNA fragment containing the HSP terminator (HSPt) 29 was amplified by PCR, with SacI and EcoRI sites attached to either end of the DNA. The amplified HSPt fragment was digested with SacI and EcoRI.…”

Section: Construction Of Plasmidsmentioning

confidence: 99%

A chimeric repressor of petunia PH4 R2R3-MYB family transcription factor generates margined flowers in torenia

Kasajima

Sasaki

2016

Plant Signaling & Behavior

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The development of new phenotypes is key to the commercial development of the main floricultural species and cultivars. Important new phenotypes include features such as multiple-flowers, color variations, increased flower size, new petal shapes, variegation and distinctive petal margin colourations. Although their commercial use is not yet common, the transgenic technologies provide a potentially rapid means of generating interesting new phenotypes. In this report, we construct 5 vectors which we expected to change the color of the flower anthocyanins, from purple to blue, regulating vacuolar pH. When these constructs were transformed into purple torenia, we unexpectedly recovered some genotypes having slightly margined petals. These transgenic lines expressed a chimeric repressor of the petunia PhPH4 gene under the control of Cauliflower mosaic virus 35 S RNA promoter. PhPH4 is an R2R3-type MYB transcription factor. The transgenic lines lacked pigmentation in the petal margin cells both on the adaxial and abaxial surfaces. Expressions of Flavanone 3-hydroxylase (F3H), Flavonoid 3 0 -hydroxylase (F3 0 H) and Flavonoid 3 0 5 0 -hydroxylase (F3 0 5 0 H) genes were reduced in the margins of these transgenic lines, suggesting an inhibitory effect of PhPH4 repressor on anthocyanin synthesis.

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“…We have tested several terminators from plant genes, and found that the terminator of the heat-shock gene of Arabidopsis thaliana shows an increase in transcription of a foreign gene by four times. 5 Among 64 different combinations of nucleotides at positions À3 to À1 of ATG initiation codon, translation efficiency varied, and the optimum sequence was (A/G)(a/c)(a/g) AUG in A. thaliana and (A/G)(u/C)(g/C)AUG in Oryza sativa. 6 Alteration of the codon of a mammalian gene to plant type is also important to increase translational activity.…”

Section: Increase Of Accumulation Of Foreign Protein In Plantsmentioning

confidence: 99%

“…7 If we choose the optimum combination of transcription and translation units, we can expect to accumulate foreign protein in high concentration; hence, we succeeded in accumulating b-glucuronidase of more than 1% of total soluble protein in Arabidopsis. 5 …”

Section: Increase Of Accumulation Of Foreign Protein In Plantsmentioning

confidence: 99%

Molecular farming: production of drugs and vaccines in higher plants

Shinmyo

Kato

2010

J Antibiot

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On the basis of developments in plant biotechnology, drug and vaccine production by higher plants can be added to microbial and animal cell culture processes. When genes encoding drug or vaccine formation under a suitable promoter are introduced into plants, these useful compounds can be economically produced from CO 2 and inorganic chemicals using sunlight. The merits and demerits of the plant process are discussed in this paper. Keywords: chloroplast transformation; glycosylation; medicinal protein; molecular farming; plant; Prof. Demain; transgene expression Although plants have been used for medicinal purposes for thousands of years, chemical and microbial medicines are being consumed more at present. Recombinant DNA technology in higher plants has opened up a new field of basic research and application in plant science. Basically, foreign genes can be introduced into any kind of plant and it is possible to produce therapeutic proteins, such as antibodies, blood products, cytokines, growth factors, hormones, vaccines and enzymes. The first recombinant plant-derived pharmaceutical protein was human serum albumin discovered in 1990 in transgenic tobacco and potato plants. 1 From an economic point of view, the production cost of biomolecules using plants is less than that of microbial and animal cell cultures, which require equipment, substrate and electric energy supply. Plants can synthesize any protein and metabolite from CO 2 and inorganic chemicals using solar energy. The production cost of immunoglobulin A by transgenic plants is estimated to be less than 1% of that by mammalian cell culture, and less than 5% of that by transgenic goats. 2

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The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells

Cited by 183 publications

References 17 publications

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

A chimeric repressor of petunia PH4 R2R3-MYB family transcription factor generates margined flowers in torenia

Molecular farming: production of drugs and vaccines in higher plants

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