The HSD‐hCG Vaccine Prevents Pregnancy in Women: Feasibility Study of a Reversible Safe Contraceptive Vaccine

Talwar, G.P.; Singh, Om; Gupta, Satish Kumar; Hasnain, Seyed E.; Pal, Rahul; Majumdar, Subeer S.; Vrati, Sudhanshu; Mukhopadhay, A.; Srinivasan, J; Deshmukh, Umesh S.; Ganga, S.; Mandokhot, A.; Gupta, Amitesh

doi:10.1111/j.1600-0897.1997.tb00207.x

Cited by 65 publications

(41 citation statements)

References 22 publications

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“…While O-linked carbohydrates are responsible for a longer circulatory half-life of the hormone, N-linked carbohydrates are required for proper folding of the molecule (Matzuk & Boime 1988). The hCG-specific antibodies (mouse monoclonal and human polyclonal) used in this study are capable of neutralising hCG and recognise a discontinuous determinant that dominates the immune response in humans (Talwar et al 1994(Talwar et al , 1997. Discontinuous epitopes are, by their nature, highly dependent on the correct folding and conformation of the molecule.…”

Section: Discussionmentioning

confidence: 99%

“…These antibodies are bioneutralising in nature and recognise a discontinuous epitope on hCG (Talwar et al 1994(Talwar et al , 1997. Analysis of cold displacement curves revealed that VV-expressed recombinant hCG bound each antibody tested with the same affinity as did native hCG (K a =2·5 10 10 M 1 to 4·7 10 10 M 1 ) (Fig.…”

Section: Expression and Characterisation Of Hcgmentioning

confidence: 99%

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High expression of human chorionic gonadotrophin beta-subunit using a synthetic vaccinia virus promoter

Gupta

Chandrasekhar²,

Pal³

et al. 2001

Journal of Molecular Endocrinology

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We have constructed a recombinant vaccinia virus to express the -subunit of human chorionic gonadotrophin ( hCG), a secretory glycoprotein that is used as an antigen for a contraceptive vaccine. The cDNA encoding the subunit was cloned under the control of a synthetic promoter that could be recognised by a vaccinia virus RNA polymerase to direct transcription. The peak expression level of hCG directed by a late synthetic promoter (Psyn) was 11·5 µg/ml, a level that was at least sixfold higher than that directed by the p7·5 early/late promoter. The expressed protein was correctly processed post-translationally such that it attained a conformation with correctly folded discontinuous epitope(s) similar to that seen in native hCG.

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Section: Discussionmentioning

confidence: 99%

Section: Expression and Characterisation Of Hcgmentioning

confidence: 99%

High expression of human chorionic gonadotrophin beta-subunit using a synthetic vaccinia virus promoter

Gupta

Chandrasekhar²,

Pal³

et al. 2001

Journal of Molecular Endocrinology

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“…Subjek masih dapat mengalami ovulasi, memproduksi hormon steroid sex secara normal, dan siklus menstruasi tetap teratur serta tidak ditemukan perdarahan yang irreguler seperti flek-flek, amenorrhea atai perdarahan tambahan. 17 Fase luteal pada wanita yang mendapat vaksin ini tidak mengalami pemanjangan durasi yang mengkonfirmasi penelitian sebelumnya pada monyet yang diberi antibodi anti-hCG untuk mencegah terjadinya implantasi embrio ke dalam endometrium. 15 Vaksin harus memiliki efektivitas sebesar >90-95%.…”

Section: β-Hcg-ttunclassified

Vaksin Human Chorionic Gonadotropin (hCG) sebagai Kandidat Kontrasepsi Imunologi pada Wanita

Darmawi

2017

J Kesehat Melayu

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ABSTRAKTercatat pada tahun 2017 ada sekitar 7,5 miliar jumlah populasi manusia dengan angka pertumbuhan 1 miliar setiap 12 tahun. Peningkatan populasi ini terjadi 95% di negara berkembang. Setiap tahun ada sekitar 80 juta wanita di seluruh dunia setiap tahunnya mengalami kehamilan yang tidak diinginkan dan sebagian besar wanita pada kelompok ini menggunakan beberapa metode kontrasepsi yang telah tersedia. Oleh karena itu, diperlukan metode kontrasepsi baru dan lebih baik yang dapat diterima, efektif, dapat dijangkau dan tersedia diberbagai negara berkembang, yaitu dengan metode kontrasepsi imunologi. Salah satu metode kontrasepsi imunologi pada wanita adalah vaksin hCG. Vaksin hCG ini pertama kali dipublikasikan oleh Talwar, et al pada tahun 1976 dan menjadi satu-satunya metode kontrasepsi imunologi yang telah melewati uji klinis fase II. hCG merupakan target potensial dan terbaik dalam mencegah kehamilan tanpa adanya efek samping yang merugikan. Berbeda dari kontrasepsi steroid yang dapat menghambat ovulasi, metode vaksin hCG tidak menganggu ovulasi dan produksi hormon steorid seks serta tidak menganggu keteraturan siklus menstruasi. Baik protein ujung C (CTP) dari β-hCG maupun β-hCGnya sendiri telah digunakan sebagai kandidat imunogen untuk mengembangkan kontrasepsi imunologi. Metode ini diketahui aman, efektif menekan angka kehamilan dan bersifat reversibel.

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“…61 The efficacy of the vaccine to prevent pregnancy was high with only one pregnancy recorded in 1224 cycles above 50 ng ⁄ mL. 4,62 These historic phase II trials, the first carried out on a potential birth control vaccine in the world, demonstrated the ability of a vaccine engendering antibodies that are competent to inactivate the bioactivity of hCG, to prevent pregnancy in sexually active women, without impairment of ovulation and derangement of menstrual regularity and bleeding profiles.…”

Section: Selective Delivery Of Cytotoxic Compounds To Tumor Cellsmentioning

confidence: 99%

Immunological Approaches Against Human Chorionic Gonadotropin for Control of Fertility and Therapy of Advanced‐Stage Cancers Expressing hCG/Subunits

Talwar¹,

Gupta²,

Shankar³

2011

American J Rep Immunol

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Citation Talwar GP, Gupta JC, Shankar NV. Immunological approaches against human chorionic gonadotropin for control of fertility and therapy of advanced‐stage cancers expressing hCG/subunits. Am J Reprod Immunol 2011; 66: 26–39 The year 2011 marks the 84th year of the discovery of human chorionic gonadotropin (hCG) by Ascheim and Zondek. Originally considered and employed as a reliable diagnostic index for pregnancy, the multiple roles of hCG as an initiator and sustainer of pregnancy are now recognized. Besides pregnancy, the expression of hCG or its subunits is observed in a number of cancers of diverse type, in particular at advanced stage. Cancers expressing hCG/subunits have poor prognosis and adverse survival. Thus, immunological approaches against hCG have applications for control of fertility and for treatment of terminal cancers. Various mechanisms by which hCG exercises its action are discussed. These include its role as autocrine growth promoter, inhibitor of apoptosis, promotor of angiogenesis, invasiveness, and protection against rejection by the immune system. The article reviews various vaccines developed for control of fertility and for therapy of advanced‐stage cancers expressing ectopically hCG/subunits. Also reviewed are the recombinant fully humanized and chimeric antibodies usable for emergency contraception, as vacation contraceptive, and as therapeutic antibodies for treatment of cancers.

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The HSD‐hCG Vaccine Prevents Pregnancy in Women: Feasibility Study of a Reversible Safe Contraceptive Vaccine

Cited by 65 publications

References 22 publications

High expression of human chorionic gonadotrophin beta-subunit using a synthetic vaccinia virus promoter

High expression of human chorionic gonadotrophin beta-subunit using a synthetic vaccinia virus promoter

Vaksin Human Chorionic Gonadotropin (hCG) sebagai Kandidat Kontrasepsi Imunologi pada Wanita

Immunological Approaches Against Human Chorionic Gonadotropin for Control of Fertility and Therapy of Advanced‐Stage Cancers Expressing hCG/Subunits

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