2000
DOI: 10.1038/sj.onc.1204010
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The HPV16 E5 oncogene inhibits endocytic trafficking

Abstract: The small hydrophobic E5 protein of Human Papillomavirus type 16 (HPV16) binds to the 16-kDa subunit of the V-H + -ATPase. This binding has been suggested to interfere with acidi®cation of late endocytic structures. We here used video microscopy, ratio imaging and confocal microscopy of living C127 ®broblasts to study the e ects of E5. Various endocytic markers including the pH-sensitive probe DM-NERF coupled to dextran, TransFluoSpheres and TRITC-concanavalin A, were applied. In E5-transfected cells, none of … Show more

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Cited by 64 publications
(53 citation statements)
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“…Additionally, microfilament depolymerization can disrupt lipid rafts (Bacia et al, 2004). The latter finding is of particular interest, since it has been reported that 16E5 induces complete disassembly of microfilaments in mouse fibroblasts (Thomsen et al, 2000). However, fluorescent labeling of filamentous actin in our HEC lines clearly shows that neither 16E5 nor 16E5(À10) perturbs the actin cytoskeleton (Figure 7a).…”
Section: E5-induced Redistribution Of Ganglioside Gm1 Is Not Mediatmentioning
confidence: 75%
“…Additionally, microfilament depolymerization can disrupt lipid rafts (Bacia et al, 2004). The latter finding is of particular interest, since it has been reported that 16E5 induces complete disassembly of microfilaments in mouse fibroblasts (Thomsen et al, 2000). However, fluorescent labeling of filamentous actin in our HEC lines clearly shows that neither 16E5 nor 16E5(À10) perturbs the actin cytoskeleton (Figure 7a).…”
Section: E5-induced Redistribution Of Ganglioside Gm1 Is Not Mediatmentioning
confidence: 75%
“…Since 16E5 inhibits endocytic vesicle trafficking [34] and KNβ3 may play a role in vesicle transport [20], it is possible that the mechanism by which 16E5 influences vesicle trafficking is dependent upon the interaction between these two proteins. Our unpublished data show that the 16E5/KNβ3 interaction plays an important role in the induction of perinuclear vacuolization, i.e., koilocytosis, a characteristic morphological feature of papillomavirus infection [35].…”
Section: Discussionmentioning
confidence: 99%
“…Failure to acidify endosomes may be responsible for the inhibition of EGF receptor degradation, thereby allowing increased receptor recycling to the membrane (Figure 3) (Straight et al, 1993;Yoshimori et al, 1991). However, a recent report suggests that HPV16 E5 a ects endocytic tra cking rather than endosome acidi®cation (Thomsen et al, 2000). Gap junction-mediated cell-cell communication is also inhibited in keratinocytes expressing the HPV16 E5 protein (Oelze et al, 1995).…”
Section: Hpv E5 and 16 Kda V-atpasementioning
confidence: 99%