“…Membranes were exposed to ®lms, (Eastman Kodak Co., Rochester, NY, USA) and the subsequent autoradiographs were then analysed using a densitometer (Bio-Rad Laboratories, Hercules, CA, USA). We have previously shown that this blotting procedure, in conjunction with the RT ± PCR conditions used, allows accurate quanti®cation of the Brn-3a and Brn-3b mRNAs relative to the constitutively expressed cyclophilin mRNA (Lillycrop et al, 1992;Ndisang et al, 1998;Morris et al, 1994).…”