The HPB-AML-I cell line possesses the properties of mesenchymal stem cells

Abstract

BackgroundIn spite of its establishment from the peripheral blood of a case with acute myeloid leukemia (AML)-M1, HPB-AML-I shows plastic adherence with spindle-like morphology. In addition, lipid droplets can be induced in HPB-AML-I cells by methylisobutylxanthine, hydrocortisone, and indomethacin. These findings suggest that HPB-AML-I is similar to mesenchymal stem cells (MSCs) or mesenchymal stromal cells rather than to hematopoietic cells.MethodsTo examine this possibility, we characterized HPB-AML-I by performing cytochemical, cytogenetic, and phenotypic analyses, induction of differentiation toward mesenchymal lineage cells, and mixed lymphocyte culture analysis.ResultsHPB-AML-I proved to be negative for myeloperoxidase, while surface antigen analysis disclosed that it was positive for MSC-related antigens, such as CD29, CD44, CD55, CD59, and CD73, but not for CD14, CD19, CD34, CD45, CD90, CD105, CD117, and HLA-DR. Karyotypic analysis showed the presence of complicated abnormalities, but no reciprocal translocations typically detected in AML cases. Following the induction of differentiation toward adipocytes, chondrocytes, and osteocytes, HPB-AML-I cells showed, in conjunction with extracellular matrix formation, lipid accumulation, proteoglycan synthesis, and alkaline phosphatase expression. Mixed lymphocyte culture demonstrated that CD3+ T-cell proliferation was suppressed in the presence of HPB-AML-I cells.ConclusionsWe conclude that HPB-AML-I cells appear to be unique neoplastic cells, which may be derived from MSCs, but are not hematopoietic progenitor cells.

Circulating osteogenic cells: Implications for injury, repair, and regeneration

“…(55) That early chondrocyte progenitors may be of myeloid origin is supported by other studies in which myeloid precursor cells can transdifferentiate into chondrocyte-like cells or MSC-like adherent cells can be established from the peripheral blood of a patient with acute myeloid leukemia. (56,57) …”

Yin and Yang of Pluripotency: Results of Analysis of Genes Overexpressed In Tumor-Initiating Cells of Krebs-2 Ascites Carcinoma

“…Четыре из указанных генов включены в эту группу с некоторыми оговорками. Для генов Cd55 и Il10 не показано непосредственное участие в формировании или поддержании стволовости нормальных плюри-/мультипотентных клеток, но показана их роль в реализации репаративных функций стволовых клеток через иммуносуппрессивное действие белковых продуктов указанных генов [212][213][214][215]. Для гена Nts участие в формировании плюрипотентного фенотипа показано лишь в случае т.н.…”

Cancer Stem Cells: Emergent Nature of Tumor Emergency

“…However, four of these genes were included in this group with some reservations. Thus, for Cd55 and Il10 , no direct contribution to the formation or maintenance of stemness of normal pluri-/multipotent cells was proved, but the essential role in the realization of the reparative functions of mesenchymal and autologous-induced pluripotent stem cells by dint of the immunosuppressive action of the protein products of these genes was demonstrated (Ardianto et al, 2010 ; Liu et al, 2012 ; de Almeida et al, 2014 ; Lee et al, 2015b ). The role of Nts in the formation of the pluripotent phenotype has been proved only in the case of the so-called induced pluripotent cells (Cai et al, 2015 ).…”