The HP10 Taenia monoclonal antibody-based ELISA detects a similar protein in the vesicular fluid of Taenia hydatigena

Cortéz, María Milagros; Rojas, Glenda; Parkhouse, R. M. E.

doi:10.1007/s11250-017-1473-7

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…29 As expected, this assay cross-reacted with T. hydatigena. Cross-reaction with T. hydatigena has been previously described by Dorny et al 30 and demonstrated in assays with HP10 Ag-ELISA 31 and B158/ B60 Ag-ELISA. 23 The present study confirms the crossreactivity, with 20 of the 22 T. hydatigena-infected pigs (90.9%) testing positive in 158C11/60H8 Ag-ELISA, 13 of which had saturating OD results (59.1%).…”

Section: Discussionmentioning

confidence: 74%

Performance of a Sandwich Antigen-Detection ELISA for the Diagnosis of Porcine Taenia solium Cysticercosis

Bustos

Ninaquispe

Rodríguez

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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The pig is the natural intermediate host of Taenia solium, a parasite causing significant burden of disease in both humans and pigs. Porcine cysticercosis is traditionally detected via tongue palpation and slaughterhouse meat inspection, both with limited sensitivity. Serum antibody detection has a better performance; however, it does not discriminate past from present infection. Serum antigen detection can demonstrate viable infection and gives a good estimate of parasitic load. This study evaluated a sandwich antigen-detection ELISA using monoclonal antibodies (MoAbs) 158C11 and 60H8 for the diagnosis of viable cysticercosis in pigs. Serum samples were used from 35 naturally T. solium cysticerci-infected pigs, 31 cysticercosis-negative pigs, and 22 pigs with Taenia hydatigena infection (to assess cross-reactions). Positive cysticercosis samples were subcategorized at necropsy according to parasitic burden as mild (1-10 viable cysts, n = 10), moderate (11-100 cysts, n = 5), or severe infection (more than 100 cysts, n = 20). This Ag-ELISA showed a sensitivity of 82.9% and a specificity of 96.8% when not considering cross-reactions with T. hydatigena. Hundred percentage of severely infected, 80% of moderately infected, and 50% of mildly T. solium-infected pigs tested positive. Twenty of 22 pigs with only T. hydatigena infections were positive, with 13 reaching saturating levels in the ELISA. The Ag-ELISA revealed the presence of live cysts and is, thus, a fairly reliable test to monitor experimental infection, response to treatment, and follow-up in animal models of cysticercosis. It should, however, be carefully interpreted when used in regions where T. hydatigena is endemic in pigs.

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Section: Discussionmentioning

confidence: 74%

Performance of a Sandwich Antigen-Detection ELISA for the Diagnosis of Porcine Taenia solium Cysticercosis

Bustos

Ninaquispe

Rodríguez

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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“…Many serological tests have been developed to diagnose both conditions. An example of E/S proteins used in the immunodiagnosis of cysticercosis is the HP10 Ag-based ELISA, which detects a high-molecular-weight secreted protein from viable metacestodes; this serological test has already been used for the diagnosis of human and porcine cysticercosis (table 5) (Cortez et al ., 2018). For taeniasis, a serological diagnostic test has been developed using the TSES33 and TSES38 proteins.…”

Section: Medical Uses (Diagnosis Vaccines Inflammation Treatment) Omentioning

confidence: 99%

Biological role of excretory–secretory proteins in endemic parasites of Latin America and the Caribbean

et al. 2019

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Neglected tropical diseases (NTDs) share certain traits: they are parasitic infections, prevailing in tropical environments and affecting marginalized sectors of the population. Six NTDs – ascariasis, cysticercosis, echinococcosis, hookworm infection, onchocerciasis and trichuriasis – all of them endemic in Latin America and the Caribbean (LAC), are analysed in this work. This review aims to discuss key information on the function of excretory/secretory (E/S) proteins from these parasites in their infectivity, pathogeny and diagnosis. The modulation of the host immune system to favour the permanence and survival of the parasite is also discussed. An updated knowledge on the function of E/S molecules in endemic parasitoses in LAC may lead to new approaches for the clinical management and diagnosis of these diseases. In turn, this could allow us to optimize their treatment and make it more affordable – a relevant goal given the economic constraints that the region is facing.

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“…For certain transmission (hypoendemic) settings characterised by flat age-(sero)prevalence profiles, especially where the FoI/seroconversion is low (Sarti et al 40 in Mexico; Kungu et al 37 in rural Ugandan production systems), it is not possible to be certain that pigs were exposed at all due to the likelihood of substantial numbers of false positives (not being possible to distinguish the infection model/process with only false-positives driving the model fit to observable data). Current serology-based diagnostics suffer from reduced specificity due to the presence of cross-reactions to T. hydatigena with the Ag-ELISA tests 68 , 69 and specifically to the GP50 band in the antibody LLGP-EITB assay 70 , 71 . The modelling approach in this paper is based on fitting directly to observed data, rather than fitting to adjusted data, to allow incorporation of uncertainty associated with the sensitivity and specificity of the respective diagnostics into the relationship between the true prevalence (a function of the catalytic models) and the observed data.…”

Section: Discussionmentioning

confidence: 99%

Force-of-infection of Taenia solium porcine cysticercosis: a modelling analysis to assess global incidence and prevalence trends

Dixon

Winskill

Harrison³

et al. 2020

Sci Rep

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The World Health Organization (WHO) called, in 2012, for a validated strategy towards Taenia solium taeniasis/cysticercosis control and elimination. Estimating pig force-of-infection (FoI, the average rate at which susceptible pigs become infected) across geographical settings will help understand local epidemiology and inform effective intervention design. Porcine cysticercosis (PCC) age-prevalence data (from 15 studies in Latin America, Africa and Asia) were identified through systematic review. Catalytic models were fitted to the data using Bayesian methods, incorporating uncertainty in diagnostic performance, to estimate rates of antibody seroconversion, viable metacestode acquisition, and seroreversion/infection loss. There was evidence of antibody seroreversion across 5 studies, and of infection loss in 6 studies measured by antigen or necropsy, indicating transient serological responses and natural resolution of infection. Concerted efforts should be made to collect robust data using improved diagnostics to better understand geographical heterogeneities in T. solium transmission to support post-2020 WHO targets.

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The HP10 Taenia monoclonal antibody-based ELISA detects a similar protein in the vesicular fluid of Taenia hydatigena

Cited by 14 publications

References 17 publications

Performance of a Sandwich Antigen-Detection ELISA for the Diagnosis of Porcine Taenia solium Cysticercosis

Performance of a Sandwich Antigen-Detection ELISA for the Diagnosis of Porcine Taenia solium Cysticercosis

Biological role of excretory–secretory proteins in endemic parasites of Latin America and the Caribbean

Force-of-infection of Taenia solium porcine cysticercosis: a modelling analysis to assess global incidence and prevalence trends

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