The house-dust mite (Dermatophagoides pteronyssinus) and the allergens it produces. Identity with the house-dust allergen

Voorhorst, R; Spieksma, F. Th. M.; Varekamp, H.; Leupen, M.J.; Lyklema, A.W.

doi:10.1016/0021-8707(67)90045-7

Cited by 421 publications

(173 citation statements)

References 4 publications

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“…This result is in accordance with previous studies 3,[5][6][7] , although the precise positive rates showed some discrepancies in each study. This reconfirmed that the most common allergen in allergic skin diseases is the house dust mite; D. farinae and D. pteronyssinus are known to be main sources of house dust allergens 11,12 . These allergens are considered to be the most important causative allergens in allergic asthma and perennial rhinitis 13,14 , and they also might be an essential causative allergen in atopic dermatitis.…”

Section: Discussionmentioning

confidence: 78%

Analysis of MAST-CLA Results as a Diagnostic Tool in Allergic Skin Diseases

et al. 2010

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Background: Urticaria and atopic dermatitis are representative allergic skin diseases that can be mediated by IgE. Measuring levels of specific IgE can be used to confirm causative agents of these skin diseases. Objective: To analyze results from the multiple allergosorbent test chemiluminescent assay (MAST-CLA), which measures specific IgE in the presence of a causative agent/allergen, in IgE-mediated skin diseases. Methods: A total of 404 patients with urticaria, atopic dermatitis or pruritus were enrolled in the present study. Positive rates of specific IgE as well as total serum IgE from the MAST-CLA were compared. Results: Positive rates of specific IgE were highest in atopic dermatitis patients, followed by urticaria, and then pruritus, with 57.0%, 37.1%, and 20.8%, respectively (p＜0.05). House dust mite species were the most common allergens in both atopic dermatitis and urticaria skin diseases. There were no differences in the overall MAST-CLA results between acute and chronic urticaria. The relative positive rate of inhalant allergen was significantly higher in adult than in child atopic dermatitis patients. Conclusion: Results from the MAST-CLA showed diversity among the three disease groups, and within each disease group, with different positive rates of specific IgE, a different mean allergen number per patient, and so on. Therefore, we concluded that MAST-CLA could be a useful diagnostic tool for various allergic skin diseases. (Ann Dermatol 22(1) 35∼40, 2010)

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Section: Discussionmentioning

confidence: 78%

Analysis of MAST-CLA Results as a Diagnostic Tool in Allergic Skin Diseases

et al. 2010

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“…We chose to study the human immune response to Dpt as hypersensitivity to this mite is the most common cause of allergic bronchial asthma in our country (16). Moreover, the main Dpt allergens have been characterized and are available commercially (17).…”

Section: Discussionmentioning

confidence: 99%

Allergic bronchial asthma due to Dermatophagoides pteronyssinus hypersensitivity can be efficiently treated by inoculation of allergen-antibody complexes.

Machiels

Somville²,

Lebrun³

et al. 1990

J. Clin. Invest.

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Antigen-antibody complexes were made from allergens of the common house dust mite, Dermatophagoides pteronyssinus (Dpt) and an excess of purified autologous specific antibodies. These complexes have been used to treat Dpt-hypersensitive patients who suffered from chronic bronchial asthma. Clinical symptoms and medication intake were followed by filling in diary cards. Peak expiratory flow, measured four times a day, was also followed. Intradermal skin tests and bronchial challenge tests were performed with allergen together with an evaluation of nonspecific bronchial reactivity. Specific IgE and IgG antibodies were assayed after separation from the bulk of serum immunoglobulins by immunoadsorption. The study was carried out over two years according to a double-blind protocol.Intradermal inoculation of antigen-antibody complexes resulted in a marked reduction of both clinical and medication scores. No systemic side-effects were observed and only mild wheal and flare reactions were noted at the injection site. The treatment showed a drastic reduction of specific skin and bronchial reactivities with only marginal effects on nonspecific bronchial reactivity. Concentrations of specific IgE antibodies decreased significantly during the first weeks of treatment and remained at these lower values throughout the study. Specific IgG antibodies actually decreased in the majority of treated patients. The total amount of allergen used in this study was < 1% of the amount currently used for conventional hyposensitization with the same allergen.These findings show that antigen-antibody complex inoculation is an efficient and safe means of treating allergic bronchial asthma and that the mechanism of action is likely to differ from conventional hyposensitization. (J. Clin. Invest.

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“…H ouse dust mites of two species, Dermatophagoides pteronyssinus and Dermatophagoides farinae, are major sources of allergens associated with allergic diseases such as asthma, rhinitis, and atopic dermatitis (1)(2)(3)(4). Group 1 and group 2 allergens are major allergens derived from house dust mites based on the frequency of patients sensitized, amount of specific IgE, and content in mite extract (4 -10).…”

mentioning

confidence: 99%

Crucial Commitment of Proteolytic Activity of a Purified Recombinant Major House Dust Mite Allergen Der p1 to Sensitization toward IgE and IgG Responses

Kikuchi

Takai²,

Kuhara³

et al. 2006

The Journal of Immunology

102

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The major proteolytic allergen derived from the house dust mite Dermatophagoides pteronyssinus, Der p1, is one of the most clinically relevant allergens worldwide. In the present study, we evaluate the contribution of the proteolytic activity and structure of a highly purified rDer p 1 to immune responses. Mice were i.p. immunized with three forms of rDer p 1 adsorbed to Alum: one enzymatically active, one treated with an irreversible cysteine protease-specific inhibitor, E-64, and one heat denatured. Immunization with E-64-treated or heat-denatured rDer p 1 elicited much less production of serum total IgE and not only rDer p 1-specific IgE but also IgGs compared with immunization with active rDer p 1. Assays for Ab-binding and its inhibition and structural analyses indicated that E-64-treated rDer p 1 retained its global structure and conformational B cell epitopes. A proliferative response and production of IL-5 by spleen cells restimulated with rDer p 1 were observed on immunization with the active rDer p 1 but not E-64-treated rDer p 1. The cells from mice immunized with heat-denatured rDer p 1 exhibited the highest levels of proliferation and production of IL-5 and IFN-γ. The results indicate that the proteolytic activity of the highly purified rDer p 1 crucially commits to the sensitization process, including both IgE and IgG responses. Additionally, we demonstrated immunogenic differences by functional or structural manipulations of the rDer p 1. The findings have implications for sensitization to this relevant allergen in humans and for the design of modified allergen-vaccines for future allergen-specific immunotherapy.

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The house-dust mite (Dermatophagoides pteronyssinus) and the allergens it produces. Identity with the house-dust allergen

Cited by 421 publications

References 4 publications

Analysis of MAST-CLA Results as a Diagnostic Tool in Allergic Skin Diseases

Analysis of MAST-CLA Results as a Diagnostic Tool in Allergic Skin Diseases

Allergic bronchial asthma due to Dermatophagoides pteronyssinus hypersensitivity can be efficiently treated by inoculation of allergen-antibody complexes.

Crucial Commitment of Proteolytic Activity of a Purified Recombinant Major House Dust Mite Allergen Der p1 to Sensitization toward IgE and IgG Responses

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