The Host-Protein-Independent Iron Uptake byTritrichomonas foetus

Tachezy, Jan; Suchan, Pavel; Schrével, Joseph; Kulda, Jaroslav

doi:10.1006/expr.1998.4327

Cited by 18 publications

(24 citation statements)

References 44 publications

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“…Likewise, there is evidence that TF binds to the surface of Trypanosoma cruzi and is subsequently internalized through an unknown mechanism [23]. The life cycles of T. vaginalis and T. foetus are limited to mucosal surfaces, and these protozoa bind LF, but not TF, in a receptor-mediated fashion [17,26]. Parasitic LF binding proteins potentially play a role in iron acquisition.…”

Section: Discussionmentioning

confidence: 99%

The Detection of Bovine Lactoferrin Binding Protein on Trypanosoma brucei

et al. 2004

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ABSTRACT. Trypanosoma brucei, the causative agent of sleeping sickness in humans, requires transferrin (TF) for growth. Therefore, T. brucei has a TF receptor that allows it to obtain iron from TF. Lactoferrin (LF), a member of the TF family protein, is an iron-binding protein that is found in most biological fluids of mammals. LF has been shown to interact with some bacteria species by specifi c receptor-ligand binding. We examined the ability of T. brucei to bind bovine LF (bLF) by using a fluorescence test, streptavidin-biotin (SAB) microplate analysis, and far Western blotting using a biotin-streptavidin system. We found that bLF bound to components of T. brucei, and that bLF hydrolysate disrupted the sites responsible for binding to parasite proteins. Furthermore, bLF, human LF, bovine TF, and ovotransferrin bound same proteins of T. brucei, which exhibited molecular masses of 40 and 43 kDa. The N-terminal amino acid sequence of the 40 kDa bLF binding protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

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Section: Discussionmentioning

confidence: 99%

The Detection of Bovine Lactoferrin Binding Protein on Trypanosoma brucei

et al. 2004

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“…Iron uptake from TF is a process dependent on the energy produced by glycolysis, as sodium fluoride affected the uptake [Tachezy et al, 1998]. The mechanism also involves extracellular iron reduction from holo-TF.…”

Section: Iron Uptake From Transferrin In T Foetusmentioning

confidence: 99%

“…This idea is supported by the inhibitory effect of BPSA (a membrane impermeable, ferrous-iron specific chelator) on iron uptake from holo-TF, as iron is originally in the ferric state in this molecule. Additionally, the presence of ascorbic acid, a strong reducing agent, stimulated iron accumulation by T. foetus from holo-TF [Tachezy et al, 1998]. Which mechanism is actually used by T. foetus to reduce holo-TF iron is unknown.…”

Section: Iron Uptake From Transferrin In T Foetusmentioning

confidence: 99%

“…Which mechanism is actually used by T. foetus to reduce holo-TF iron is unknown. Iron released from holo-TF could be acquired by a mechanism related or identical to that used for acquisition from the low molecular weight iron chelator nitrilotriacetic acid (Fe-NTA) because these processes displayed similar kinetics and susceptibility to various agents [Tachezy et al, 1998]. Iron uptake from Fe-NTA by this microorganism also depends on iron reduction and is better characterized.…”

Section: Iron Uptake From Transferrin In T Foetusmentioning

confidence: 99%

“…Iron acquisition from TF seems to be independent of endocytosis because lysosomotropic bases such as ammonium chloride and chloroquine acting as inhibitors of endosome acidification did not decrease iron accumulation from TF [Tachezy et al, 1998]. However, work from Affonso shows that endocytosis of TF by T. foetus actually takes place [Affonso et al, 1994].…”

Section: Iron Uptake From Transferrin In T Foetusmentioning

confidence: 99%

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Transferrin Binding Proteins as a Means to Obtain Iron in Parasitic Protozoa

Reyes-López¹,

Serrano-Luna²,

Piña-Vázquez³

et al. 2012

Binding Protein

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Transient invagination of flagella by Tritrichomonas foetus

Granger

Warwood

Benchimol³

et al. 2000

Parasitol Res

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We describe an experimental system for the study of rapid and reversible formation of pseudocysts, which are spherical forms that lack a true cyst wall, by Tritrichomonas foetus, a trichomonad parasite of the bovine genitourinary tract. It highlights the dynamics of the plasma membrane and cytoskeleton of this parasite, which is perpetually devoid of any sort of protective cell wall, and can reflect a responsive survival mechanism. We have found that cooling of axenic cultures of T. foetus from their normal 37 degrees C to below about 16 degrees C can trigger pseudocyst formation. The three anterior flagella and the single recurrent flagellum can be fully internalized within 1 3 min at 37 degrees C, with the axonemes and flagellar membranes remaining intact within the cell body. Electron microscopy confirms that the internalized flagella are surrounded by a separate membrane. At 37 degrees C the internalized flagella can resume beating movements and become externalized as quickly as within 10 min. We have begun to elucidate the mechanisms of this unusual phenomenon, characterizing its temperature dependence and exploring the effects of agents that interfere with various aspects of the cytoskeleton, phagocytosis, endocytosis, and exocytosis.

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The Host-Protein-Independent Iron Uptake byTritrichomonas foetus

Cited by 18 publications

References 44 publications

The Detection of Bovine Lactoferrin Binding Protein on Trypanosoma brucei

The Detection of Bovine Lactoferrin Binding Protein on Trypanosoma brucei

Transferrin Binding Proteins as a Means to Obtain Iron in Parasitic Protozoa

Transient invagination of flagella by Tritrichomonas foetus

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