The host plant strongly modulates acaricide resistance levels to mitochondrial complex II inhibitors in a multi-resistant field population of Tetranychus urticae

Njiru, Christine; Vandenhole, Marilou; Jonckheere, Wim; Wybouw, Nicky; Van Leeuwen, Thomas

doi:10.1016/j.pestbp.2023.105591

Cited by 3 publications

(3 citation statements)

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“…The variants with the largest diUerences in AF were located within a cluster of TuENaC genes (TuENaC13, TuENaC14, TuENaC15, TuENaC17), a complex, possibly copy number variable region, as suggested by DNA coverage. Another noteworthy candidate is tetur01g00490 from the DOG family, previously shown to be highly upregulated in the VR-BE line when adapted to tomato compared to when adapted to bean (Njiru et al, 2023). We observed multiple missense variants within this gene and observed selection towards the Pol-R allele in the low-performing bulk, resulting in a loss of VR-BE alleles.…”

Section: Genomic Responses Linked To Performance On Tomatomentioning

confidence: 68%

“…To map loci associated with reproductive performance on tomato, we used two laboratory T. urticae lines with red body colouration: VR-BE, a field line obtained from tomato plants (Njiru et al, 2023), and Pol-R, an iso-female line developed previously in the laboratory. Lines were kept under standard conditions on detached bean leaves surrounded by wet-cotton wool.…”

Section: F2 Backcross Approachmentioning

confidence: 99%

“…This gene was previously reported to be significantly upregulated in multiple T. urticae populations feeding on tomato (e.g., log2FC of 4.78 in Snoeck et al, 2018;log2FC of 3.46 in Wybouw et al, 2015). Notably, Njiru et al, 2023 propagated the VR-BE line (used here for the F2 backcross approach) on tomato and on bean, and found that the tomato line had a higher expression of CYP392B3 (log2FC of 4.2). In our study, this gene did not show constitutive upregulation or altered induced expression upon tomato adaptation.…”

Section: Molecular Determinants Of Mite Adaptationmentioning

confidence: 99%

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Independent genetic mapping experiments identify diverse molecular determinants of host adaptation in a generalist herbivore

Villacis-Perez,

Graeve,

Beer

et al. 2024

Preprint

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Interactions between plants and herbivores promote evolutionary change. Studying the evolution of herbivore mechanisms aimed to cope with diUerent host plant species is a critical intersection between evolutionary biology and sustainable pest management. Generalist herbivores are of particular interest, as hybridization between genetically distinct populations can increase the standing genetic variation and therefore the adaptive potential of the species.Tetranychus urticaeis a generalist arthropod known for its adaptive potential, evidenced in its immense host range and ability to develop metabolic resistance to xenobiotics. However, the molecular underpinnings associated with the potential of host adaptation and the consequences of host adaptation in this and many other pests remain elusive. Here, we use two independent, empirical approaches to identify and map the genetic basis of host plant performance and adaptation in genetically distinct populations ofT. urticae. In the first approach, we subject a genetically diverse mite population to tomato selection and map genomic regions linked to the phenotypic evolution of increased reproductive performance. In the second approach, we map genomic regions responsible for performance on tomato by comparing the genomes of pooled individuals from a F2 backcross between populations with high and low reproductive performance. Both approaches revealed specific and shared genomic regions associated with host plant performance and adaptation and key candidate genes were identified. Our findings highlight the power of spider mite genetic approaches to identify the complex genetic basis of host adaptation in a generalist herbivore.

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Section: Genomic Responses Linked To Performance On Tomatomentioning

confidence: 68%

Section: F2 Backcross Approachmentioning

confidence: 99%

Section: Molecular Determinants Of Mite Adaptationmentioning

confidence: 99%

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Independent genetic mapping experiments identify diverse molecular determinants of host adaptation in a generalist herbivore

Villacis-Perez,

Graeve,

Beer

et al. 2024

Preprint

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Variation in Pesticide Toxicity in the Western Honey Bee (Apis mellifera) Associated with Consuming Phytochemically Different Monofloral Honeys

Liao,

Wu,

Berenbaum

2024

J Chem Ecol

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Insecticide toxicity to insect herbivores has long been known to vary across different host plants; this phenomenon has been widely documented in both foliage-feeders and sap-feeders. Species-specific phytochemical content of hostplant tissues is assumed to determine the pattern of induction of insect enzymes that detoxify insecticides, but specific phytochemicals have rarely been linked to host plant-associated variation in pesticide toxicity. Moreover, no studies to date have examined the effects of nectar source identity and phytochemical composition on the toxicity of insecticides to pollinators. In this study, we compared LD50 values for the insecticide bifenthrin, a frequent contaminant of nectar and pollen in agroecosystems, in the western honey bee, Apis mellifera, consuming three phytochemically different monofloral honeys: Nyssa ogeche (tupelo), Robinia pseudoacacia (black locust), and Fagopyrum esculentum (buckwheat). We found that bifenthrin toxicity (LD50) values for honey bees across different honey diets is linked to their species-specific phytochemical content. The profiles of phenolic acids and flavonoids of buckwheat and locust honeys are richer than is the profile of tupelo honey, with buckwheat honey containing the highest total content of phytochemicals and associated with the highest bifenthrin LD50 in honey bees. The vector fitting in the ordination analysis revealed positive correlations between LD50 values and two honey phytochemical richness estimates, Chao1 and Abundance-based Coverage Estimator (ACE). These findings suggest unequal effects among different phytochemicals, consistent with the interpretation that certain compounds, including ones that are rare, may have a more pronounced effect in mitigating pesticide toxicity.

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Beauveria bassiana Vuillemin İzolatlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerine Ovisidal Etkisi

Uysal,

Obut,

Uzun Yiğit

2024

Afyon Kocatepe University Journal of Sciences and Engineering

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Bu çalışma ile Beauveria bassiana’nın BY2 (Burdur, Yeşilova), BIM-001 (Isparta, Merkez) ve IGÇ (Isparta, Merkez) izolatlarının üç farklı dozunun (1x106 spor/ml, 1x107 spor/ml ve 1x108 spor/ml) Tetranychus urticae üzerinde ovisidal etkisinin laboratuvar koşullarında belirlenmesi hedeflenmiştir. Ticari preparat Nostalgist (Agrobest Grup Ltd. Şti, Beauveria bassiana Bb-1 ırkı) ve kontrol olarak Tween 80 (% 0,1) içeren saf su kullanılmıştır. Denemeler plastik petrilerde (9 cm) 5 tekerrürlü olarak yürütülmüştür. Fasulye yaprak diskleri (4 cm) petri plakasında tutulan steril suya doymuş pamuk üzerinde yerleştirilmiştir. Daha sonra 10 ergin birey, yaprak disklere aktarılarak yumurta bırakmalarına izin verilmiştir. Her bir petri plakasında 20 yumurta/yaprak diski olacak şekilde ayarlanmıştır. Her bir entomopatojen fungus izolatına ait spor süspansiyonu 4 bar basınçta püskürtme sağlayan modifiye edilmiş bir düzenek yardımıyla 10 sn süre ile yumurta içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak yumurta ölümleri kaydedilmiştir. Sonuç olarak, B. bassiana BIM-001, BY2 ve IGÇ izolatlarının 1x108 spor/ml konsantrasyonları uygulandıktan 7 gün sonra T. urticae yumurtalarında meydana gelen ölüm oranları sırasıyla %71, %66 ve %59 olarak belirlenmiştir. Beauveria bassiana strain Bb-1 uygulaması diğer entomopatojen fungus izolatlarına ait uygulamalardan istatistiksel olarak anlamlı bulunmuştur. Bununla birlikte B. bassiana BIM-001 1x108, 1x107 spor/ml ve B. bassiana BY2 1x108 spor/ml uygulamalarındaki ölüm oranları arasında fark olmadığı saptanmıştır (p˂0.005).

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The host plant strongly modulates acaricide resistance levels to mitochondrial complex II inhibitors in a multi-resistant field population of Tetranychus urticae

Cited by 3 publications

References 73 publications

Independent genetic mapping experiments identify diverse molecular determinants of host adaptation in a generalist herbivore

Independent genetic mapping experiments identify diverse molecular determinants of host adaptation in a generalist herbivore

Variation in Pesticide Toxicity in the Western Honey Bee (Apis mellifera) Associated with Consuming Phytochemically Different Monofloral Honeys

Beauveria bassiana Vuillemin İzolatlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerine Ovisidal Etkisi

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