The HOS test and its relationship to fertility in the stallion

Neild, D.; Chaves, Marı́a Graciela; Flores, Maximino Luna; Miragaya, Marcelo; González, Eva López; Agüero, A.

doi:10.1046/j.1439-0272.2000.00357.x

Cited by 49 publications

(42 citation statements)

References 4 publications

(5 reference statements)

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“…Nevertheless, even though the potential fertility of semen is difficult to predict accurately on the basis of a single assay, we agree with the statement of Neild et al (2000) that the development of simple, inexpensive, more comprehensive assays that cover different sperm functions continues to be of interest. In this way, unlike to what is necessary to perform the evaluation with fluorescence probes, hypo-osmotic test requires fewer materials and can be quickly accomplished in field, demanding only a phase contrast microscope.…”

Section: Discussionsupporting

confidence: 79%

“…As we known motility has a high correlation with fertility, which makes the execution of these two tests on the pre-freezing breeding soundness exam so important. Neild et al (2000), also evaluated if HOST in raw semen could be correlated with stallion fertility and concluded that there is a good correlation with HOST progressive motility of the raw semen (r = 0.82, P = 0.0001). Despite HOS test was not correlated with the percentage of pregnant mares (r = 0.08, P = 0.75), there was a tendency to correlate with the number of services per pregnancy (r = -0.40; P = 0.08).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of hypo-osmotic swelling test and supra vital staining technique as indicators for donkey semen freezability

Oliveira¹,

Teixeira²,

Pignataro³

et al. 2017

PHK

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Summary: Reproductive biotechnologies such as cooling and freezing semen are employed in order to obtain genetic enhancement in domestic animal breeds. However, cryopreservation of donkey semen, as in several other domestic mammalian species, has not yet reached a standard procedure that provides satisfactory and repeatable results, as in bulls. Despite there are over 43 million donkeys worldwide, there are only few studies about donkey semen preservation. The aim of this study was to evaluate the predictive potential of hypo-osmotic test and the supra vital staining in raw semen to be used as indicators for donkey semen freezability. Ejaculates were collected twice per week from 10 fertile donkey stallions. Volume, appearance (color and density), concentration, total motility, morphology, sperm viability (supra vital staining -eosin-nigrosin-EN) and plasma membrane integrity (hypo-osmotic swelling test -HOST) were evaluated in raw semen. After dilution the samples were centrifugated and sperm pellets were resuspended in a freezing extender to a concentration of 50 ×10 6 cells/mL. Aliquots were packed into 0.5 mL straws and placed in the refrigerator (5°C) for 20 min. Subsequently these straws were kept above liquid nitrogen for 20 min, then plunged into nitrogen and stored in a holding tank. Post-thaw analyzes consisted in computerized analysis of sperm movement characteristics, sperm viability (EN), plasma membrane integrity (HOST and fluorescent probe) and acrosome membrane integrity using fluorescent probe. High correlation was found between EN (0.93) and HOST (0.69) in raw semen when compared with total motility after frozen-thawed semen. Low correlation was found between EN and HOST when compared with plasma and acrosomal membrane integrity in post-thawed with fluorescent probes. Since sperm motility is one of the key parameters to evaluate the quality of frozen semen, hypo-osmotic test and supra vital staining can be considered good predictive indicators in raw semen for donkey semen freezability.Keywords: Equus Asinus, cryopreservation, eosin-nigrosin, plasma membrane integrity, semen, reproduction Citation: Arruda de Oliveira R., Batista Silva Teixeira A., Almeida Pignataro T., Freitas M. L., Castro Alves Teixeira H., de Oliveira Carvalho J., Alves Nune Dode M., Pivato I., Budik S. (2017) Evaluation of Hypo-osmotic swelling test and supra vital staining technique as indicators for donkey semen freezability. Pferdeheilkunde 33,[159][160][161][162][163][164]

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Evaluation of hypo-osmotic swelling test and supra vital staining technique as indicators for donkey semen freezability

Oliveira¹,

Teixeira²,

Pignataro³

et al. 2017

PHK

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“…Após esse período, 15 μL dessa solução foram avaliados em câma-ra úmida, sob microscopia de contraste de fase, em aumento de 400x. Foram contadas 100 células, considerando-se a presença ou ausência de cauda enrolada (Neild et al 2000). O cálculo da reação da reação hiposmótica foi realizado pela fórmula: HOST= (% de caudas enroladas após o HOST) -(% de caudas enroladas antes do HOST) conforme Melo & Henry (1999).…”

Section: Avaliação Da Motilidade Total (Mt) E Progressiva (Mp)unclassified

Características do sêmen a fresco e descongelado de garanhões da raça Nordestina

et al. 2015

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RESUMO.-Este estudo descreveu as características seminais, da membrana plasmática e do acrossoma de espermatozoide congelado/descongelado de 19 ejaculados de garanhões da raça Nordestina. Os aspectos analisados incluíram os parâmetros físicos do sêmen fresco; a motilidade e a longevidade do sêmen diluído e descongelado; a morfologia espermática, integridade funcional e estrutural da membrana plasmática do espermatozoide e a habilidade de ligação do espermatozoide à membrana perivitelina da gema do ovo de galinha do sêmen descongelado. This paper describes the seminal characteristics of the plasma membrane of frozenthawed sperm. Nineteen ejaculates of Nordestino horse breed. The aspects analyzed in the physical parameters of fresh semen were total and progressive motility and your longevity after dilution or thawed; sperm morphology, functional and structural integrity of the plasma membrane of the sperm and the sperm-binding ability to the perivitelline membrane of the yolk (MPV) after thawed. The variables were assessed by ANOVA with post hoc test of Student Newman-Keuls test (P<0.05). The total and progressive motility were higher in diluted semen than thawed (P<0.05). The average percentage of the major, minor and total defects was lower than the limit recommended by the CBRA. The percentage of reactive to hypo-osmotic swelling test was 14.21±1.12%, the intact membrane detected by supravitally test was 62.22±9.06% and the SYBR-14 was 81.47±26.9. The ability of sperm to bind to the MPV after thawing semen was 230.39±57.09. The total and progressive motility at time 0 min of termo resistance test was higher than to 150 minutes (P<0.05), and no difference was observed in the times 10 and 30 minutes. The results demonstrate that the use of additional laboratory tests help in the process of evaluation of samples, making possible to obtain more reliable and accurate information. Although cryopreservation has caused decrease in sperm motility and was used diluents with amides to diluted and cryopreservation protocol and this minimized the osmotic damage to sperm cells and maintained the morphological, functional and structural integrity of the plasma membrane of the sperm. These results are a reference for future studies since there are no comparative data on this breed.

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“…Also, the percentages of sperm motility, swollen heads, and coiled tails of BB and WNZ rabbit spermatozoa in hypo-osmotic solution at 37 o C for 20 min were significantly (P<0.01) higher in winter (14.4, 42.7, and 38.5%) than those obtained during the summer season (9.8, 33.7, and 26.6%), respectively. When understanding the changes that spermatozoa are subjected to in a hypo-osmotic environment, if we accept the suggestion that these changes indicate a functionally intact plasma membrane, and that this is necessary for normal spermatozoal function, then, within a semen sample, the more cells with swollen plasma membranes, the better the potential sperm quality (Neild et al, 2000). In addition, it was evident that the advancement of the incubation time at 37 0 C for up to 20 min decreased the grades and percentages of progressive sperm motility and increased the percentages of spermatozoa with swollen heads and coiled tails for all experimental groups with regards to main effects or interactions.…”

Section: Response Of Spermatozoa To Hostmentioning

confidence: 99%

Seasonal effects on semen quality in Black Baladi and White New Zealand rabbit bucks.

Safaa¹,

Emarah²,

Saleh³

2010

World rabbit sci.

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A total of 32 sexually mature rabbit bucks (at 6 th month of age) were used in this experiment to study the effects of breed (Black Baladi -BB-vs. White New Zealand -WNZ-) and season (summer vs. winter) on libido and seminal parameters. The experimental design was completely random with four groups arranged factorially (two breeds and two seasons) with eight rabbits in each group and three months per season. The results obtained from this study indicated that, libido (14.5 vs. 21.9 sec) and physical semen characteristics represented by the volume of semen per ejaculate without gel fractions (0.70 vs. 0.49 mL), sperm-cell concentration (703 vs. 597×10 6 /mL), total sperm output (513 vs. 293×10 6 /ejaculate), sperm abnormalities (11.6 vs. 14.0%), acrosomal damages (8.6 vs. 11.5%), dead spermatozoa (13.9 vs. 16.0%), and advanced sperm motility (63.2 vs. 57.1%) were signifi cantly (P<0.01) better on BB rabbit bucks than on WNZ breed. In addition, these parameters proved to be signifi cantly (P<0.01) better in winter season (15.8 sec, 0.68 mL, 702×10 6 /mL, 487×10 6 /ejaculate, 10.7%, 8.4%, 12.7%, and 65.6%, respectively) than in summer (20.6 sec, 0.52 mL, 598×10 6 /mL, 319×10 6 /ejaculate, 15.0%, 11.7%, 17.3%, and 54.7%, respectively). Following the hypo-osmotic swelling test (HOST) at 75 mOsmol/L during incubation at 37 o C for 20 min, the percentages of sperm motility, swollen spermatozoa, and spermatozoa with coiled tails were higher for BB bucks (16.98, 44.08, and 39.13) than for the WNZ breed (7.2, 32.3, and 26.0). This was also the case in the winter season (14.4, 42.7, and 38.5) when compared to summer (9.8, 33.7, and 26.6), respectively. We concluded that, under Egyptian conditions, both libido and semen quality in BB bucks seems to be better than those displayed by the WNZ bucks. Nevertheless, overall semen characteristics were better in winter than in the summer season.

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The HOS test and its relationship to fertility in the stallion

Cited by 49 publications

References 4 publications

Evaluation of hypo-osmotic swelling test and supra vital staining technique as indicators for donkey semen freezability

Evaluation of hypo-osmotic swelling test and supra vital staining technique as indicators for donkey semen freezability

Características do sêmen a fresco e descongelado de garanhões da raça Nordestina

Seasonal effects on semen quality in Black Baladi and White New Zealand rabbit bucks.

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