The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.

Josephy, P. David; Eling, Thomas E.; Mason, Ronald P.

doi:10.1016/s0021-9258(18)34832-4

Cited by 825 publications

(408 citation statements)

References 11 publications

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“…Peroxidase mimicking activity of CoSe 2 MS was principally evaluated by using 3, 3’, 5, 5’‐tetramethylbenzidine (TMB) as a chromogenic substrate which appears blue upon oxidation in presence of CoSe 2 MS and H 2 O 2 . The absorption intensity of the blue‐colored oxidation product increases with time which could be recorded using UV‐Vis spectrophotometer at 653 nm [40] . The colorless TMB substrate gives the oxidized product of TMB in presence of CoSe 2 MS and H 2 O 2 with absorbance enhancement at 653 nm and 370 nm with respect to time (Figure 4a).…”

Section: Resultsmentioning

confidence: 97%

Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

et al. 2021

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In this investigation, we report a new and greener self‐assembly of CoSe2 microsphere (CoSe2 MS) without assistance of harmful reducing and structure‐directing agents (SDA). Intrinsic peroxidase mimicking activity of synthesized CoSe2 MS was evaluated for 3,3,5,5′‐tetramethylbenzidine as a substrate by monitoring the absorbance of oxidized product at 653 nm. The smaller Michalis Menten constant (Km) for CoSe2 MS catalyzed reaction (0.371 mM), than the natural Horse Radish Peroxidase (HRP) enzyme, signifies the high substrate affinity of CoSe2 MS than HRP. Further, intrinsic peroxidase activity was assessed using emission intensity enhancement of terephthalic acid (TA) in presence of CoSe2 MS and H2O2 with time due to the production of .OH radicals and subsequent hydroxylation of TA. CoSe2 MS was further employed for organic dyes degradation in presence of H2O2, where cationic and anionic dyes degraded within 120 min with the highest degradation efficiency of 99.45 % for malachite green.

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Section: Resultsmentioning

confidence: 97%

Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

et al. 2021

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“…The POX-Act test is based on the oxidation of the chromogen substrate 3,5,3′5’-tetramethylbenzidine (TMB) by the reaction produced between the horseradish peroxidase (HRP) added in the solution and some of the ROS present in the sample (Fig. 5 ) [ 118 ]. Blue coloured products corresponding to the TMB cation free radical (absorbance maximum at 653 nm) are generated [ 118 ].…”

Section: Main Textmentioning

confidence: 99%

“…5 ) [ 118 ]. Blue coloured products corresponding to the TMB cation free radical (absorbance maximum at 653 nm) are generated [ 118 ]. Results are calculated from the standard linear curve using known H 2 O 2 concentrations by subtracting the first absorbance reading from the second [ 101 ].…”

Section: Main Textmentioning

confidence: 99%

Spectrophotometric assays for evaluation of Reactive Oxygen Species (ROS) in serum: general concepts and applications in dogs and humans

Rubio

Cerón

2021

BMC Vet Res

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Reactive oxygen species (ROS) are reactive compounds derived from oxygen. In biological systems, an excessive amount of ROS can cause oxidative damage to biological macromolecules being involved in different diseases. Several assays have been developed in the last 30 years for ROS evaluation. The objective of this article will be to provide an update about the spectrophotometric methods currently used in the assessment of ROS in serum. The chemical basis of four different techniques will be reviewed, and examples of their possible applications will be provided. A particular emphasis about the practical applications of these assays in the dog will be made, but selected information about their use in humans will also be presented for comparative purposes, following a One-Health approach. The information about the spectrophotometric assays presented in this paper should be interpreted with caution once limited information about them is available yet, and further studies should be performed to clarify what they measure and their clinical application. Ideally, when applied to evaluate a sample’s oxidative status, they should be incorporated in a panel of analytes where other oxidants, antioxidants, and biomarkers of inflammation were also included.

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“…Since TMB + is quite unstable in an acidic environment, it further oxidizes to TMB 2+ when the second electron is transferred; TMB 2+ is stable in acidic conditions, exhibiting a yellow color and a maximum absorption peak at 453 nm (Fig. 3a) [71].…”

Section: Veri Cation Of the Peroxidase-like Activity Of Sio 2 @Au Npsmentioning

confidence: 99%

Synthesis of Finely Controllable Sizes of Au Nanoparticles on a Silica Template and Their Nanozyme Properties

Seong

Kim

et al. 2021

Preprint

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The precise synthesis of fine-sized nanoparticles is critical for realizing the advantages of nanoparticles for various applications. We developed a technique for preparing finely controllable sizes of gold nanoparticles (Au NPs) on a silica template using the seed-mediated growth and interval dropping methods. These Au NPs, embedded on silica nanospheres (SiO2@Au NPs), possess peroxidase-like activity as nanozymes and have several advantages over other nanoparticle-based nanozymes. We confirmed their peroxidase activity; in addition, factors affecting the activity were investigated by varying the reaction conditions such as concentrations of tetramethyl benzidine and H2O2, pH, particle amount, reaction time, and termination time. We found that SiO2@Au NPs are highly stable under long-term storage and reusable for five cycles. Our study, therefore, provides a novel method for controlling the properties of nanoparticles and for developing nanoparticle-based nanozymes.

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The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.

Cited by 825 publications

References 11 publications

Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

Spectrophotometric assays for evaluation of Reactive Oxygen Species (ROS) in serum: general concepts and applications in dogs and humans

Synthesis of Finely Controllable Sizes of Au Nanoparticles on a Silica Template and Their Nanozyme Properties

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The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.

Cited by 825 publications

References 11 publications

Self‐assembled CoSe2 Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

Self‐assembled CoSe2 Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

Spectrophotometric assays for evaluation of Reactive Oxygen Species (ROS) in serum: general concepts and applications in dogs and humans

Synthesis of Finely Controllable Sizes of Au Nanoparticles on a Silica Template and Their Nanozyme Properties

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Product

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Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes

Self‐assembled CoSe₂ Microspheres with Intrinsic Peroxidase Mimicking Activity for Efficient Degradation of Variety of Dyes