2001
DOI: 10.1128/jb.183.5.1680-1687.2001
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The Hook Gene ( flgE ) Is Expressed from the flgBCDEF Operon in Rhodobacter sphaeroides : Study of an flgE Mutant

Abstract: In this work we identified the flgE gene encoding the flagellar hook protein from Rhodobacter sphaeroides. Our results show that this gene is part of a flagellar cluster that includes the genes flgB, flgC, flgD, flgE, and flgF. Two different types of mutants in the flgE gene were isolated, and both showed a Fla ؊ phenotype, indicating the functionality of this sequence. Complementation studies of these mutant strains suggest that flgE is included in a single transcriptional unit that starts in flgB and ends in… Show more

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Cited by 19 publications
(21 citation statements)
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“…Motility was not observed for the transconjugant cells when they were examined in swarming plates containing 0.25% agar or directly with the light microscope (data not shown). In agreement with previous observations, the motility the LC1 strain was recovered when a plasmid expressing the flgE1 gene was introduced into this strain (4). The FlgE2 protein expressed in the LC1 strain carrying the pRKflgE2 plasmid was detected by Western blotting (Fig.…”
Section: Resultssupporting
confidence: 71%
See 1 more Smart Citation
“…Motility was not observed for the transconjugant cells when they were examined in swarming plates containing 0.25% agar or directly with the light microscope (data not shown). In agreement with previous observations, the motility the LC1 strain was recovered when a plasmid expressing the flgE1 gene was introduced into this strain (4). The FlgE2 protein expressed in the LC1 strain carrying the pRKflgE2 plasmid was detected by Western blotting (Fig.…”
Section: Resultssupporting
confidence: 71%
“…To generate the flgE2::aadA allele, we amplified the flgE2 gene with oligonucleotides flgE2F (5Ј-CCGATGGCGGTGCGAAACAAC-3Ј) and flgE2R (5Ј-GGTCGCATTCA TCTGCTGCAC-3Ј) using chromosomal DNA from WS8. The 2,080-bp PCR product was cloned in pTZ19R; the aadA gene was amplified by PCR as described previously (4), and the product was cloned in the SalI site of the flgE2 gene. The resultant allele, flgE2::aadA, was subcloned into pJQ200mp18 and introduced into SP18 fla2ϩ by conjugation.…”
Section: Methodsmentioning
confidence: 99%
“…This is consistent with electron micrographs showing that the R. sphaeroides hook is straight, not curved like E. coli (27). Recent models of the flagellar motor have proposed elastic links between stators and the cell wall, each with a stiffness of approximately 200 pNnm/rad 2 (24,28).…”
Section: Discussionsupporting
confidence: 75%
“…The finding that FlgE was posttranscriptionally regulated in response to flagellar assembly led us to examine a possible role of mRNA processing in the expression of the 9.9-kbp class 2 flgBCDEFGHIJKL operon. Also, cleavage of the flgBCDEF GHIJ operon has been implicated in posttranscriptional regulation of the flgE gene in Rhodobacter sphaeroides (4,18).…”
Section: Resultsmentioning
confidence: 99%
“…The stoichiometry of the FlgE protein is 5 to 20 times higher than that of other flgB-L operon proteins. Northern blot analysis of Rhodobacter sphaeroides flgE suggested a role for mRNA processing in the regulation of flgB-J operon expression and identified two different flgE-containing transcripts (4).…”
mentioning
confidence: 99%