The Homeoproteins MAB-18 and CEH-14 Insulate the Dauer Collagen Gene col-43 from Activation by the Adjacent Promoter of the Spermatheca Gene sth-1 in Caenorhabditis elegans

Bando, Toshikazu; Ikeda, Tatsuji; Kagawa, Hiroaki

doi:10.1016/j.jmb.2005.01.045

Cited by 15 publications

(16 citation statements)

References 29 publications

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“…For the rescue experiments, we obtained the following promoter sequences for each gene by PCR using N2 genomic DNA as a template: a lag-2 promoter P lag-2 containing 5 kb of upstream sequence, with the first codon specifically expressed in DTCs; a lim-7 promoter P lim-7 containing 3 kb of upstream sequence, with the first exon and intron predominantly expressed in gonadal sheath cells (Voutev et al , 2009); and a sth-1 promoter P sth-1 containing 1.3 kb of upstream sequence, with the first codon expressed in the spermatheca and uterus (Bando et al , 2005). Promoter fragments were inserted into the pFX_EGFPT vector using the TA cloning method described by Gengyo-Ando et al (2006) to generate P lag-2 ::egfp , P lim-7 ::egfp , and P sth-1 ::egfp plasmids.…”

Section: Methodsmentioning

confidence: 99%

GPI-anchor synthesis is indispensable for the germline development of the nematodeCaenorhabditis elegans

Murata

Nomura

Dejima

et al. 2012

MBoC

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Glycosylphosphatidylinositol (GPI)-anchor attachment is one of the most common posttranslational protein modifications. Using the nematode Caenorhabditis elegans, we determined that GPI-anchored proteins are present in germline cells and distal tip cells, which are essential for the maintenance of the germline stem cell niche. We identified 24 C. elegans genes involved in GPI-anchor synthesis. Inhibition of various steps of GPI-anchor synthesis by RNA interference or gene knockout resulted in abnormal development of oocytes and early embryos, and both lethal and sterile phenotypes were observed. The piga-1 gene (orthologue of human PIGA) codes for the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex, which catalyzes the first step of GPI-anchor synthesis. We isolated piga-1–knockout worms and found that GPI-anchor synthesis is indispensable for the maintenance of mitotic germline cell number. The knockout worms displayed 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Using cell-specific rescue of the null allele, we showed that expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. These results clearly demonstrate that GPI-anchor synthesis is indispensable for germline formation and for normal development of oocytes and eggs.

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Section: Methodsmentioning

confidence: 99%

GPI-anchor synthesis is indispensable for the germline development of the nematodeCaenorhabditis elegans

Murata

Nomura

Dejima

et al. 2012

MBoC

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“…Although the CE1(bs258) ortholog was conserved within the upstream region of the pat-10 ortholog (CBG10771) and had a CE1(bs258) homology of 62.0% in the C. briggsae genome, the C46H11.6 ortholog (CBG09116) was not located in the upstream region of the pat-10 ortholog. As suggested elsewhere gene pairs are rarely conserved between the two genomes (Felix, 2004;Bando et al, 2005). Moreover, only a few repeat families are shared, suggesting that most elements were acquired after the two sister species diverged or that the two species are undergoing rapid genome evolution (Stein et al, 2003).…”

Section: Evolution and Biological Significance Of The Ce1 Elementsmentioning

confidence: 85%

A novel non-coding DNA family in Caenorhabditis elegans

Takashima

Bando

Kagawa

2007

Gene

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A novel non-coding DNA family in Caenorhabditis elegansKeywords: repetitive element; palindrome; gene/element association; enhancer; genome organization. -2 - Yasuo AbstractMany repetitive elements, for example, SINEs, LINEs, LTR-retrotransposons and other SSRs are dispersed throughout eukaryotic genomes. To understand the biological function of these repetitive elements is of great current research interest. In this study, we report on the identification of a novel non-coding DNA family, designated CE1 family, in the nematode C. elegans genome. Some CE1 elements constituted a large palindrome sequence. The CE1 elements were interspersed at 95 sites in the C. elegans genome. Most of the CE1 elements were associated with, or are within, protein-coding genes. The sequence of the CE1 elements indicated that some could form a hairpin structure. One of the CE1 family, CE1(bs258), is located in the first intron of a novel gene, C46H11.6 which encodes a PDZ/DHR/GLGF domain protein. In gfp and lacZ reporter gene assays the CE1(bs258) element appeared to behave as an enhancer element for the expression of C46H11.6 but no effect on the expression of the opposite direction gene, pat-10 which encodes the body-wall muscle troponin C. The CE1(bs258) RNA transcript was detected by RT-PCR even when CE1(bs258) was located in an intron. We conclude that CE1 elements are involved in the expression of adjacent genes and are therefore selectively retained in the C. elegans genome. We discussed a biological function of the CE1(bs258) having many transcription factor-binding sites.-3 -

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“…To independently verify this result, we used a second spermatheca-specific promoter from the sth-1 gene (Bando et al, 2005) to drive DPL-1 expression. Injection of sth-1::gfp:dpl-1 DNA also resulted in two extrachromosomal lines, and both lines displayed DPL-1 expression in the nucleus of spermathecal cells (Supplemental Figure S2B).…”

Section: Resultsmentioning

confidence: 99%

DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans

Chi

Reinke

2009

Mechanisms of Development

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Proper coordination of oogenesis, ovulation, and fertilization is essential for successful reproduction. In C. elegans, a strong loss-of-function mutation in dpl-1, which encodes a subunit of the E2F heterodimeric transcription factor EFL-1/DPL-1, causes severe defects during ovulation and fertilization. Here we demonstrate that the somatic gonad structure and sheath cell contraction rate appear normal in dpl-1 mutants, but that dilation of the spermatheca valve does not occur properly, causing oocytes to become trapped in the proximal gonad arm and enter endomitosis. This ovulation defect can be partially suppressed by increasing the activity of ITR-1, an inositol triphosphate receptor in the spermatheca that promotes dilation in response to IP3 signaling. Tissue-specific rescue experiments demonstrate that expression of DPL-1 in germ cells but not the spermatheca can restore both ovulation and fertilization in dpl-1 mutants, indicating that the absence of DPL-1 likely disrupts a pro-ovulation signal originating in the oocyte that in turn stimulates the spermatheca. Moreover, we found that expression of a single EFL-1/DPL-1-responsive gene, rme-2, in the germ line of dpl-1 mutants significantly rescues ovulation, but not fertilization. Instead, other EFL-1/DPL-1-responsive genes function to promote successful fertilization. We propose that DPL-1 acts with EFL-1 in developing oocytes to directly regulate a transcriptional program that couples the critical events of ovulation and fertilization.

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The Homeoproteins MAB-18 and CEH-14 Insulate the Dauer Collagen Gene col-43 from Activation by the Adjacent Promoter of the Spermatheca Gene sth-1 in Caenorhabditis elegans

Cited by 15 publications

References 29 publications

GPI-anchor synthesis is indispensable for the germline development of the nematodeCaenorhabditis elegans

GPI-anchor synthesis is indispensable for the germline development of the nematodeCaenorhabditis elegans

A novel non-coding DNA family in Caenorhabditis elegans

DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans

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