The Hole-Board Test in Mutant Mice

Lalonde, Robert; Strazielle, C.

doi:10.1007/s10519-022-10102-1

Cited by 5 publications

(4 citation statements)

References 159 publications

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“…In mice, the infarct area includes the cortex, striatum, thalamus, hypothalamus, and hippocampus [32][33][34][35]. The hippocampal formation is involved in both types of learning [24,36]. Detailed analysis revealed that the ventral CA1 and the dorsal CA2 played a unique role in social memory [37,38].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Ficus Platyphylla-Induced Hypothermia on Long-term Functional Recovery after Ischaemic Stroke

Becker,

Helmuth,

Chindo

2023

OBM Neurobiol

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A stroke is a cerebrovascular disease that results from a blockage in the blood supply to part of the brain or a burst blood vessel in the brain. It is the second leading cause of death worldwide, with an annual mortality rate of about 5.5 million. Antithrombotic therapies have failed to provide a cure for this debilitating cerebrovascular disorder, and hypothermia is gaining interest as a novel strategy for the management of stroke. In this study, we evaluated the effects of Ficus platyphylla-induced hypothermia on long-term functional recovery after ischaemic stroke. Histomorphological analysis of the brain demonstrated pathological alterations in the ipsilateral hemisphere of all animals. Animals treated before or immediately after permanent occlusion of the middle cerebral artery (MCAO) had significantly smaller infarct sizes than those given saline. Surgery and treatment did not affect locomotor activity. There were no significant differences between the groups of mice in terms of parameters associated with situational anxiety, including the number of arm changes and percentile time spent on open arms. There were no significant differences between groups regarding the number of buried marbles and sociability. Surgery and treatment did not affect social recognition, but a significant interaction between surgery and treatment was observed. The time mice remained on the rota rod was relatively similar for all groups tested, with no significant differences related to surgery and treatment, nor was there any surgery/treatment interaction. A learning effect represented by a decrease in exploratory activity was observed irrespective of surgery and treatment, and there was no surgery/treatment interaction. The results suggest that Ficus platyphylla-induced hypothermia could be beneficial to long-term functional recovery after ischaemic stroke.

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Section: Discussionmentioning

confidence: 99%

“…The hole-board test is recognized and accepted for anxiety and spatial memory [23][24][25]. The Acti-Mot apparatus (TSE, Bad Homburg, Germany) was utilized for this test.…”

Section: Hole Boardmentioning

confidence: 99%

Effects of Ficus Platyphylla-Induced Hypothermia on Long-term Functional Recovery after Ischaemic Stroke

Becker,

Helmuth,

Chindo

2023

OBM Neurobiol

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“…The test setup consists of 16 holes with a diameter of 2.2 cm on the oor. The holes are round and placed inside a square or rectangular arena (10). The number of times the mice dip their heads into these holes for 5 minutes is recorded, and their emotional state, anxiety and stress response are evaluated (11).…”

Section: Hole Boardmentioning

confidence: 99%

“…The hole board test measures head dipping and defecation in mice. Some evidence suggests that animals are curious but fearful in unfamiliar environments (Lalonde & Strazielle, 2022;File & Wardill, 1975). We found that miR-124* male and female mice defecated more than controls (Fig.…”

Section: Behavioural Observationsmentioning

confidence: 99%

Dynamic Interplay Between miR-124-3p and EGF in Regulating The 'Giant' Phenotype by RNA Signalling Mechanism

BAYRAM,

Bayram,

Suranli

et al. 2024

Preprint

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Epigenetic mechanisms and RNA signalling profoundly impact body growth during the early stages of embryonic development. Epigenetic modifications' critical role in determining which genes are activated or suppressed, thereby influencing the development. RNA molecules, like microRNAs, regulate gene expression, control cell fate, and coordinate cellular processes crucial for proper growth and development. These complex molecular processes work harmoniously to create the intricate symphony of early embryonic development, which lays the foundation for the organism's future growth and function. Mouse embryos that are microinjected with miR-124-3p (miR-124*) exhibit a significantly 'giant' phenotype before and after birth. Behavioural test results showed that miR-124* mice were more cognitively active. miR-124 is highly expressed in the brain; however, the molecular mechanism leading to the phenotypic changes mediated by miR-124-3p is unknown. This study aimed to investigate the role of epidermal growth factor (EGF) in developing a distinct phenotype ('giant and cognitive') in miR-124* mice. We established neurosphere (NS) culture from the hippocampus at E19.5 using EGF. In miR-124*, Doublecortin (Dcx), Neurod1 (12 days old NS) and Sox8, Sox9, Sox10 (21 days old NS) are significantly overexpressed. Our results suggest that the addition of EGF likely leads to overexpression of transcription factors such as Sox8, Sox9 and Sox10, while it decreased the expression of neurogenesis marker Dcx and neuronal differentiation marker Neurod1 when the culture period is extended up to 21 days in miR-124* NS. Following the microinjection of miR-124-3p into the embryo at the one-cell stage, EGF could have been activated and suppressed the expression of miR-124-3p, leading to early neurogenesis and neuronal differentiation in embryos. It led to the earlier emergence of mice with cognitively active and 'giant' phenotypes.

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The PDE4 inhibitor apremilast modulates ethanol responses in Gabrb1-S409A knock-in mice via PKA-dependent and independent mechanisms

Blednov,

Shawlot,

Homanics

et al. 2024

Neuropharmacology

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The Hole-Board Test in Mutant Mice

Cited by 5 publications

References 159 publications

Effects of <i>Ficus Platyphylla</i>-Induced Hypothermia on Long-term Functional Recovery after Ischaemic Stroke

Effects of <i>Ficus Platyphylla</i>-Induced Hypothermia on Long-term Functional Recovery after Ischaemic Stroke

Dynamic Interplay Between miR-124-3p and EGF in Regulating The 'Giant' Phenotype by RNA Signalling Mechanism

The PDE4 inhibitor apremilast modulates ethanol responses in Gabrb1-S409A knock-in mice via PKA-dependent and independent mechanisms

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