The HIV-1 Cell Entry Inhibitor T-20 Potently Chemoattracts Neutrophils by Specifically Activating the N-Formylpeptide Receptor

Hartt, Jennifer K.; Liang, Thomas S.; Sahagún-Ruíz, Alfredo; Wang, Jiming; Gao, Ji-Liang; Murphy, Philip M.

doi:10.1006/bbrc.2000.2846

Cited by 36 publications

(25 citation statements)

References 24 publications

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“…A number of pathogen-derived peptides have been recently reported to be agonists of FPR and FPRL2. These include T-20 for FPR (46) and the H. pylori-derived peptide Hp(2-20) for FPRL2 (35). Pathogens have notorious abilities to subvert the host immune system (47) and the chemoattractant system, which is one of the most important targets of this modulation of host immune response by pathogens.…”

Section: Discussionmentioning

confidence: 99%

The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Inhibits Human Monocyte-Derived Dendritic Cell Maturation via Formyl Peptide Receptor and Formyl Peptide Receptor-Like 2

Kang¹,

Lee

Kim

et al. 2005

The Journal of Immunology

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Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm) has been reported to stimulate monocytes, neutrophils, and dendritic cells (DCs). However, although WKYMVm has been reported to function as a DC chemoattractant, its role on DC maturation has not been examined. In this study, we investigated the effects of WKYMVm on human DC maturation. The costimulation of DCs with WKYMVm and LPS dramatically inhibited LPS-induced IL-12 production, CD86 and HLA-DR surface expression, and DC-mediated T cell proliferation. However, DC phagocytic activity was increased by WKYMVm stimulation. These findings demonstrate that WKYMVm inhibits DC maturation by LPS. In terms of the mechanism underlying DC maturation inhibition by WKYMVm, we found that LPS-induced DC maturation was negatively regulated by WKYMVm-stimulated ERK activity. Moreover, the costimulation of DCs with WKYMVm and LPS dramatically inhibited the LPS-induced accumulations of IL-12 mRNA, thus suggesting that WKYMVm inhibits LPS-induced IL-12 production at the transcriptional level. We also found that DCs express two WKYMVm receptors, formyl peptide receptor (FPR) and FPR-like 2 (FPRL2). In addition, formyl-Met-Leu-Phe (a FPR ligand), Trp-Lys-Tyr-Met-Val-Met, Hp(2–20) peptide, and F2L (three FPRL2 ligands) inhibited LPS-induced IL-12 production in DCs. Taken together, our findings indicate that the activations of FPR and FPRL2 inhibit LPS-induced DC maturation, and suggest that these two receptors should be regarded as important potential therapeutic targets for the modulation of DC maturation.

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Section: Discussionmentioning

confidence: 99%

The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Inhibits Human Monocyte-Derived Dendritic Cell Maturation via Formyl Peptide Receptor and Formyl Peptide Receptor-Like 2

Kang¹,

Lee

Kim

et al. 2005

The Journal of Immunology

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“…HEK 293 cells (ATCC, Manassas, VA) were cultured in DMEM plus 10% FBS. HEK 293 cells stably expressing FPR1 (23) were selected with 2 mg/ml G418 (Cellgro). Cells were cultured at 37°C in 5% CO 2 and 100% humidity.…”

Section: Methodsmentioning

confidence: 99%

The Leukocyte Chemotactic Receptor FPR1 Is Functionally Expressed on Human Lens Epithelial Cells

Schneider

Weaver

Gaur

et al. 2012

Journal of Biological Chemistry

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“…responses in mice (Gao et al, 1999;Hartt et al, 2000;Liang et al, 2000;Tiffany et al, 2001). Activating FPR1 via its specific agonist, N-formyl-methionyl-leucyl-phenylalanine (fMLF) induced the chemotactic migration of phagocytic cells, such as monocytes and neutrophils.…”

Section: Trp-lys-tyr-met-val-met Stim Ulates Phagocytosis Via Phosphomentioning

confidence: 99%

Trp-Lys-Tyr-Met-Val-Met stimulates phagocytosis via phospho-lipase D-dependent signaling in mouse dendritic cells

Lee¹,

Kang²,

Jo³

et al. 2004

Exp Mol Med

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The HIV-1 Cell Entry Inhibitor T-20 Potently Chemoattracts Neutrophils by Specifically Activating the N-Formylpeptide Receptor

Cited by 36 publications

References 24 publications

The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Inhibits Human Monocyte-Derived Dendritic Cell Maturation via Formyl Peptide Receptor and Formyl Peptide Receptor-Like 2

The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Inhibits Human Monocyte-Derived Dendritic Cell Maturation via Formyl Peptide Receptor and Formyl Peptide Receptor-Like 2

The Leukocyte Chemotactic Receptor FPR1 Is Functionally Expressed on Human Lens Epithelial Cells

Trp-Lys-Tyr-Met-Val-Met stimulates phagocytosis via phospho-lipase D-dependent signaling in mouse dendritic cells

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