The “Histoplasmosis Porto Alegre manifesto”—Addressing disseminated histoplasmosis in AIDS

Pasqualotto, Alessandro C.; Queiroz‐Telles, Flávio; Chebabo, Alberto; Leitão, T. M. J. S.; Falci, Diego Rodrigues; Xavier, Melissa Orzechowski; Bay, Mônica Baumgardt; Sprinz, Eduardo; Lana, Daiane Dalla; Vincentini, Adriana P; Damasceno, Lisandra Serra; Schwarzbold, Alexandre Vargas; Ferreira, Paulo Roberto Abrão; Godoy, Cassia Miranda; Vidal, José Ernesto; Basso, Rossana Patrícia; Driemeyer, Cândida; Aquino, Valério Rodrigues; Severo, Cecília Bittencourt; Ferreira, Marcelo Simão; Bastos, Claudilson; Prohaska, Filipe; Melo, Marineide; Cavassin, Francelise Bridi; Lacerda, Marcus; Soares, Renata de Bastos Ascenço; Zancopé‐Oliveira, Rosely Maria; Teixeira, Marcus de Melo; Pérez, Freddy; Cáceres, Diego H.; Rodríguez-Tudela, Juan Luis; Chiller, Tom; Colombo, Arnaldo Lopes

doi:10.1371/journal.pntd.0010960

Cited by 5 publications

(4 citation statements)

References 18 publications

(23 reference statements)

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“…Traditionally, growth of Histoplasma on culture from a clinical specimen has been the gold standard for diagnosis [ 10 ]. Despite this, the overall sensitivity of culture is quite low for some types of histoplasmosis (50 to 85 percent) and varies widely [ 11 ]. Culture can be performed on a number of specimens, including sputum, blood, bone marrow, or almost any tissue suspected to be involved [ 1 ].…”

Section: Current State Of Diagnosticsmentioning

confidence: 99%

The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis

Villareal

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Pasqualotto

et al. 2023

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Histoplasmosis is caused by Histoplasma capsulatum and, although endemic in large parts of the world, is often underrecognized in many locations. In addition to underrecognition, inadequate availability of diagnostic tests is a major contributor to poor outcomes in disseminated disease in people with HIV. For those with advanced HIV and disseminated disease, antibody testing is less useful. Culture and histopathology can be useful in this situation, but each has limitations, including variable sensitivity by site and, in the case of culture, the need for a biosafety level three laboratory and a long period of growth. Antigen testing has proven useful for disseminated histoplasmosis due to the excellent sensitivity of urine. Yet, turnaround is slower than ideal due to use in a limited number of centers. The development of lateral flow assays has the potential to make for true rapid point-of-care assays for histoplasmosis, but in order to meet that promise, the tests must be widely available and affordable.

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Section: Current State Of Diagnosticsmentioning

confidence: 99%

The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis

Villareal

Price

Pasqualotto

et al. 2023

JoF

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“…Early detection of this disease is quintessential in this high-risk population. Traditional diagnostic methods like microscopy and histopathology have limitations in sensitivity and specificity [42]. Laboratory cultures, though considered the gold standard, are slow and not always feasible due to safety requirements.…”

Section: Diagnostic Capabilitymentioning

confidence: 99%

“…The introduction of antigen detection tests, which are quick and reliable, has revolutionized diagnosis, especially for disseminated histoplasmosis. PCR-based approaches also hold promise [42]. A recent article by Caceres et al in 2019, more commonly known as the Manus Declaration, investigated the current situation of histoplasmosis in the region.…”

Section: Diagnostic Capabilitymentioning

confidence: 99%

Fungal Infections in the Caribbean: A Review of the Literature to Date

Gousy,

Adithya Sateesh,

Denning

et al. 2023

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The most common fungal infections reported from the Caribbean include dermatophytosis, candidiasis, pneumocystis, aspergillosis, histoplasmosis, and cryptococcosis. The Caribbean is hyperendemic for histoplasmosis, with high population exposures. Fungal infections are a significant public health problem in the Caribbean, with rates varying depending on the specific country or region. In Trinidad and Tobago, the fungal burden accounts for 3.3% of the 1.4 million population, while in Jamaica, with a population of 2.9 million, over 57,600 people suffer from fungal infections each year. A study in the Dominican Republic estimated that approximately 221,027 (2%) of over 10 million people have a serious fungal infection. Fungal infections accounts for 21.9% of all skin infections in Haiti. The diagnosis of fungal infections in the Caribbean can be challenging, as access to laboratory testing and specialized medical services is limited in many areas. Access to antifungal medications can also be a challenge in some areas, and antifungal resistance has been reported.

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“…In North America, the disease is highly prevalent in the United States, in regions of the Mississippi and Ohio river valleys, although cases are still underdiagnosed, even in countries with high Gross Domestic Product (GDP) [ 4 ]. In Brazil, the number of people infected by H. capsulatum has been constantly increasing over the last few years, especially in immunocompromised patients [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets

Almeida

Roque

Felice

et al. 2023

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Histoplasma capsulatum is a thermodymorphic fungus that causes histoplasmosis, a systemic mycosis that presents different clinical manifestations, ranging from self-limiting to acute lung infection, chronic lung infection and disseminated infection. Usually, it affects severely immunocompromised patients although immunocompetent patients can also be infected. Currently, there are no vaccines to prevent histoplasmosis and the available antifungal treatment presents moderate to high toxicity. Additionally, there are few options of antifungal drugs. Thus, the aim of this study was to predict possible protein targets for the construction of potential vaccine candidates and predict potential drug targets against H. capsulatum. Whole genome sequences from four previously published H. capsulatum strains were analyzed and submitted to different bioinformatic approaches such as reverse vaccinology and subtractive genomics. A total of four proteins were characterized as good protein candidates (vaccine antigens) for vaccine development, three of which are membrane-bound and one is secreted. In addition, it was possible to predict four cytoplasmic proteins which were classified as good protein candidates and, through molecular docking performed for each identified target, we found four natural compounds that showed favorable interactions with our target proteins. Our study can help in the development of potential vaccines and new drugs that can change the current scenario of the treatment and prevention of histoplasmosis.

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The “Histoplasmosis Porto Alegre manifesto”—Addressing disseminated histoplasmosis in AIDS

Cited by 5 publications

References 18 publications

The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis

The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis

Fungal Infections in the Caribbean: A Review of the Literature to Date

Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets

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