The histopathological, cytopathological and ultrastructural effects of carbaryl on gills of Oreochromis niloticus (Linnaeus, 1758)

Uğurlu, Pelin; Satar, Elif İpek; Çiçek, Tarık

doi:10.1016/j.etap.2019.103217

Cited by 9 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, carbaryl tends to enter the human body via food chain to induce toxicity, as also confirmed by its presence in the blood of farmers (Li et al, 2019; Soomro & Seehar, 2008). Concerning the toxicity in non‐target species, carbaryl causes histopathological, cytopathological, and ultrastructural anomalies in Oreochromis niloticus (Uğurlu et al, 2019). Eisenia fetida and Metaphire posthuma reared in the presence of carbaryl exhibited histological changes (Saxena et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

View full text Add to dashboard Cite

Pesticides have adverse effects on the cellular functionality, which may trigger myriad of health consequences. However, pesticides‐mediated toxicity in the endothelial cells (ECs) is still elusive. Hence, in this study, we have used human umbilical vein endothelial cells (HUVECs) as a model to quantify the cytotoxicity and genotoxicity of four pesticides (methomyl, carbaryl, metalaxyl, and pendimethalin). In the MTT assay, HUVECs exposed to methomyl, carbaryl, metalaxyl, and pendimethalin demonstrated significant proliferation inhibition only at higher concentrations (500 and 1000 μM). Likewise, neutral red uptake (NRU) assay also showed proliferation inhibition of HUVECs at 500 and 1000 μM by the four pesticides, confirming lysosomal fragility. HUVECs exposed to the four pesticides significantly increased the level of intracellular reactive oxygen species (ROS). Comet assay and flow cytometric data exhibited DNA damage and apoptotic cell death in HUVECs after 24 h of exposure with methomyl, metalaxyl, carbaryl, and pendimethalin. This is a first study on HUVECs signifying the cytotoxic–genotoxic and apoptotic potential of carbamate insecticides (methomyl and carbaryl), fungicide (metalaxyl), and herbicide (pendimethalin). Overall, these pesticides may affect ECs functions and angiogenesis; nonetheless, mechanistic studies are warranted from the perspective of vascular biology using in vivo test models.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…Cytopathological indicators are crucial for monitoring and evaluating the toxicity of aquatic ecosystems [20][21][22][23][24][25][26]. Prolonged exposure to toxic agents often does not cause death directly; however, the structure and function of vital organs are affected, jeopardizing the individual population.…”

Section: Introductionmentioning

confidence: 99%

Impact of Chlorpyrifos on Cytopathological Indices in Mangrove Crab, Episesarma tetragonum (Fabricius)

Ravi

Athisuyambulingam

Kanagaraj

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

Chlorpyrifos is an organophosphate insecticide occurring in aquatic ecosystems. Due to exposure to xenobiotics, several harmful effects on aquatic organisms are noticed worldwide. Mangrove crabs are an ecologically important aquatic invertebrate species in food web interactions and in the mangrove ecosystem. Therefore, this study aimed to evaluate the cytotoxic effects of chlorpyrifos on the mangrove crab, Episesarma tetragonum. Crabs were exposed to 0.0294 and 0.0588 ppm of chlorpyrifos for 7 and 28 days. Cytopathologic effects on the gill, hepatopancreas, and muscle were investigated, and observations were compared with a control group. The results suggest that chlorpyrifos induces time- and concentration-dependent cytopathological alternations in the gill and exhibited epithelial lifting, oedema, necrosis, and a fusion of secondary lamellae and haemorrhage. The deceased hepatopancreas showed infiltration, a large lumen formation, and the disappearance of haemocytes, while the muscle tissue showed atrophy, necrosis, a wavy appearance, an accumulation of granular material between muscle fibres, and fragmentation in a mangrove crab. This study shows the great potential of cytopathological investigations, allows us to assess the sensitivity of various aquatic animal species to potentially dangerous compounds, and calculates safe concentrations with which to reduce pesticide use.

show abstract

Sodium chloride against Dawestrema cycloancistrium in juvenile Arapaima gigas

Affonso

Queiroz

Ono³

et al. 2022

Aquacult Int

View full text Add to dashboard Cite

The aim of the present study was to assess the use of sodium chloride (NaCl) against monogenoid infestations in pirarucu (Arapaima gigas). Two assays were conducted with pirarucu juveniles, the rst comprising an in vitro exposure to 8; 9; 10 and 11 g.L − 1 NaCl and comparison to controls not exposed to NaCl, and the second, an in vivo exposure to NaCl at the same concentrations applied in the in vitro exposure. The best in vitro results were observed for one hour exposures at 9, 10 and 11 g.L − 1 NaCl, resulting in 60% and 100% parasite mortality, respectively. In vivo exposures to 8 and 10 g.L − 1 NaCl were 36 and 22% e cient, respectively, following two hours of exposure, with no mortality. Some dosedependent changes were observed in exposed sh, such as decreased hemoglobin and HCM values at the highest NaCl concentration compared to the other NaCl concentrations and the control group. More frequent gill tissue alterations were observed compared to the control group at the highest NaCl concentrations (10 and 11 g.L − 1 ). Decreased mean (p < 0.05) water pH values and increased mean electrical conductivity were noted compared to the control group. Our ndings suggest that NaCl exhibits low toxicity towards pirarucu and anthelmintic activity to the monogenoid Dawestrema cycloancistrium, directly in uenced by exposure time and concentration.

show abstract

The histopathological, cytopathological and ultrastructural effects of carbaryl on gills of Oreochromis niloticus (Linnaeus, 1758)

Cited by 9 publications

References 58 publications

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Impact of Chlorpyrifos on Cytopathological Indices in Mangrove Crab, Episesarma tetragonum (Fabricius)

Sodium chloride against Dawestrema cycloancistrium in juvenile Arapaima gigas

Contact Info

Product

Resources

About