The Histone H3–like TAF Is Broadly Required for Transcription in Yeast

Moqtaderi, Zarmik; Keaveney, Marie; Struhl, Kevin

doi:10.1016/s1097-2765(00)80165-3

Cited by 106 publications

(122 citation statements)

References 53 publications

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“…Factors that preferentially repress T Cdirected transcription have also been identified (e.g., see Collart and Struhl 1994). In addition, some TAFs were found to be required for T C -dependent transcription but not T R -dependent transcription (Moqtaderi et al 1996(Moqtaderi et al , 1998. These findings, along with the observation that TAF dependence correlates with TAF occupancy at promoters (Kuras et al 2000;Li et al 2000), suggest that TFIID interacts with T C but not with T R .…”

Section: Tata 1 Versus Tatamentioning

confidence: 91%

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Butler¹,

Kadonaga²

2002

Genes Dev.

531

433

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Section: Tata 1 Versus Tatamentioning

confidence: 91%

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Butler¹,

Kadonaga²

2002

Genes Dev.

531

433

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“…Unexpectedly, initial studies revealed that the mutation or depletion of seven TAF II s individually resulted in gene-specific defects, and not global reductions in transcription Moqtaderi et al, 1996;Walker et al, 1996). In contrast, genetic analysis of other TFIID subunits that have sequence similarities to the core histones has shown that they are required for the transcription of a larger number of genes (Apone et al, 1998;Holstege et al, 1998;Michel et al, 1998;Moqtaderi et al, 1998;Natajaran et al, 1998;Sanders et al, 1999;Reese et al, 2000). The interpretation of these results is complicated by the presence of certain TAF II s in the SAGA histone acetyltransferase complex (Grant et al, 1998), and their function in multiple complexes may partially explain the expanded requirement for these TAF II s in transcription (Lee et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of TAF68/61 reveals links to cell cycle regulators

Reese

Green

2001

Yeast

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In yeast, inactivation of certain TBP-associated factors (TAF II s) results in arrest at specific stages of the cell cycle. In some cases, cell cycle arrest is not observed because overlapping defects in other cellular processes precludes the manifestation of an arrest phenotype. In the latter situation, genetic analysis has the potential to reveal the involvement of TAF II s in cell cycle regulation. In this report, a temperature-sensitive mutant of TAF68/61 was used to screen for high-copy dosage suppressors of its growth defect. Ten genes were isolated: TAF suppressor genes, TSGs 1-10. Remarkably, most TSGs have either a genetic or a direct link to control of the G 2 /M transition. Moreover, eight of the 10 TSGs can suppress a CDC28 mutant specifically defective for mitosis (cdc28-1N) but not an allele defective for passage through start. The identification of these genes as suppressors of cdc28-1N has identified four unreported suppressors of this allele. Moreover, synthetic lethality is observed between taf68-9 and cdc28-1N. The isolation of multiple genes involved in the control of a specific phase of the cell cycle argue that the arrest phenotypes of certain TAF II mutants reflect their role in specifically regulating cell cycle functions.

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“…This core structure was suggested to depend upon the HFD, a structural motif shared by the core histones (22) and many other proteins (70). Nine of 14 yeast Tafps appear to contain HFDs, and subsets of these can form dimers with specific partner HFD Tafps (3,6,9,23,24,29,45,47,80). Indeed, Tan and colleagues (66) have described the formation and characterization of such an octameric complex comprised of four HFD-Tafps, a dimer of Taf6p-Taf9p bound to a dimer of Taf4p-Taf12p.…”

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confidence: 99%

Molecular and Genetic Characterization of a Taf1p Domain Essential for Yeast TFIID Assembly

Singh

Bland

Weil

2004

Molecular and Cellular Biology

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Yeast Taf1p is an integral component of the multiprotein transcription factor TFIID. By using coimmunoprecipitation assays, coupled with a comprehensive set of deletion mutants encompassing the entire open reading frame of TAF1, we have discovered an essential role of a small portion of yeast Taf1p. This domain of Taf1p, termed region 4, consisting of amino acids 200 to 303, contributes critically to the assembly and stability of the 15-subunit TFIID holocomplex. Region 4 of Taf1p is mutationally sensitive, can assemble several Tafps into a partial TFIID complex, and interacts directly with Taf4p and Taf6p. Mutations in Taf1p-region 4 induce temperature-conditional growth of yeast cells. At the nonpermissive temperature these mutations have drastic effects on both TFIID integrity and mRNA synthesis. These data are consistent with the hypothesis that Taf1p subserves a critical scaffold function within the TFIID complex. The significance of these data with regard to TFIID structure and function is discussed.TFIID, one of several multiprotein general transcription factors required for RNA polymerase II (PolII)-catalyzed mRNA gene transcription, is comprised of the TATA-binding protein (TBP) and 14 distinct TBP-associated factors (TAFs or Tafps) that range in M r from 17,000 to 150,000 in Saccharomyces cerevisiae (62,63,65,75). These TFIID subunits share significant sequence conservation with their metazoan orthologs (1,22,26). In vitro, PolII needs six general transcription factors, TFIIA, -B, -D, -E, -F, and -H, to form functional preinitiation complexes (PICs) (12, 51, 54); TFIID plays several critical roles in this process. First, TFIID functions during promoter recognition by binding directly to the promoter (67,76,77). On promoters containing a TATA box, Tafps help stabilize TBP binding to the TATA element via interaction with TATA-proximal initiator DNA sequences (69). Tafp-DNA interactions may be particularly important for TFIID binding to promoters that lack a canonical TATA element and contain TATA-proximal initiator and distal promoter element sequences (13,15,42,69,76). Second, Tafps can function as coactivators during transcriptional activation by making direct contacts with specific activators leading to an increase in PolII PIC formation (1, 16). Finally, Taf1p contains several distinct enzymatic activities, those of histone acetyltransferase (HAT) (46), protein kinase (19), and ubiquitin ligase (55). These enzymatic activities presumably modify proteins that stimulate PIC formation and/or function, leading to PolII transcription initiation.In most contexts, the Tafp subunits of TFIID are essential for survival, as Tafp inactivation or depletion results in cessation of specific mRNA synthesis and loss of cell viability (3,4,33,34,56,59,64,65,78). Because of these critical roles, the composition, organization, assembly, structure, and function of the TFIID complex have been topics of great interest. TBP appears to be incorporated into the TFIID complex primarily through its interaction with the biparti...

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The Histone H3–like TAF Is Broadly Required for Transcription in Yeast

Cited by 106 publications

References 53 publications

The RNA polymerase II core promoter: a key component in the regulation of gene expression

The RNA polymerase II core promoter: a key component in the regulation of gene expression

Genetic analysis of TAF68/61 reveals links to cell cycle regulators

Molecular and Genetic Characterization of a Taf1p Domain Essential for Yeast TFIID Assembly

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